Tag: Western blot

Immunoassays: Protein Arrays vs. ELISA and Westerns

Studies of biological systems have expanded beyond the ‘one gene, one protein’ paradigm to the field of proteomics, or studying large numbers of proteins that act in a concert of complex biological systems. Thus in the post-genome era, technology has been driven to deliver multiplex assays that allow us to monitor multiple proteins in parallel, … Continued

Conserve Reagents with Miniature Western Blot Reaction Chambers

SecureSeal Hybridization Chambers were originally developed to perform hybridizations on specimens affixed to glass slides or to perform microarray incubations with limited volumes (SecureSeal References). The peel- and- stick adhesive chambers can be created in various sizes to accommodate different samples, including partial membrane blots from protein mini-gels (shown here).  Recently we have investigated the … Continued

From Western Blot to Protein Array with Super G Blocking Buffer

Results obtained from Western blots can be highly variable and dependent not only on the specific antigen/antibody pairs used, but also on other reagents in the protocol such as blocking buffer. Not surprisingly, no one “universal” blocking reagent works well with all proteins. Thus choice of blocking reagent should be assessed for optimal results and having … Continued

Super G Blocking Reagent for Western Blots

Although Super G blocking reagent was developed and optimized for fluorescent endpoint detection on nitrocellulose film slides, we have found that it is also an effective blocker for Western blot applications. Data presented below illustrate the effectiveness of Super G blocking reagent in typical Western blot experiments using nitrocellulose membranes by reducing background staining and … Continued