Tag: SYPRO Ruby

Sensitive Detection of Total Protein on RPPA using Fast Green

Reverse phase protein arrays (RPPA) are designed for sensitive quantitation of a target protein in a very small sample of tissue lysate, and multiplex analysis of many samples in one experiment. This technology holds great promise for the identification and analysis of critical biomarkers for disease states in patient samples and for high-throughput drug screening … Continued

Protein Staining Nitrocellulose using SYPRO® Ruby

Reverse phase protein arrays (RPPA) offer great promise for the identification and analysis of critical biomarkers for disease states in patient samples and for high-throughput drug screening using cultured cell systems.  Porous nitrocellulose (NC) film slides continue to be the preferred substrate for RPPA due to their high protein binding capacity and many functional advantages. … Continued