Protein crystallization is a key assay for structural studies of proteins. The protocols for crystallization of protein are challenging due to the stringent requirement for pure samples and control of environmental conditions during the crystallization process. Vapor diffusion using hanging drop is a preferred method for obtaining quality crystals with…
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Grace Bio-Labs microarray surface chemistry is based on the well-known protein-binding properties of nitrocellulose. A range of different formulations have…
Antigen-Capture Assay; Antibody Capture Assay; Coronavirus Antigen Array; Epitope-mapping; Biomarker Discovery and Validation; Immunogen Discovery;
Epoxy Microarray Slides provide a uniform substrate for a variety of DNA/RNA-based diagnostic applications.
DNA/Oligonucleotide Microarray ; microRNA Microarray ; Single Nucleotide Polymorphism (SNP) Analysis ; Gene Expression Profiling;
Grace Bio-Labs microarray regents have been specifically formulated to achieve the full potential of porous nitrocellulose, accelerating experimental design and…
Antigen-Capture Assay ; Antibody Capture Assay; RPPA- Reverse Phase Protein ; Microarray Laser micro-dissection; RRPA Epitope-mapping ; Biomarker Discovery and Validation; Immunogen Discovery;
Photostable nanoparticles arrayed on glass slides for calibration of fluorescence imaging systems and quantitative analysis.
Calibration of Microarray Scanners ; Quantitative Microarray Analyses Microscope Focal Plane Adjustment; Microscope Focal Plane Adjustment;
ProPlate® re-usable, multi-well chambers are available in a wide variety of formats to fit standard 1 x 25 x 75…
Microarray ; Hybridization and Incubation; ELISA; PCR and NGS;
CoverWell™ incubation chambers are reusable, easy to apply chambers that attach without the use of adhesive. CoverWells™ enclose a large…
Reverse Transfection Microarray; DNA Microarray; In-situ hybridization; Immunohistochemistry;
Silicone Isolators allow researchers to isolate specimens using removable hydrophobic barriers. They may be used to isolate cells grown in…
SecureSeal™ Hybridization Chambers are thin, silicone-gasketed chambers providing optimal surface-to-volume fluid dynamics for hybridization assays on large or multiple specimens…
HybriWell™ Sealing System bonds securely to a microscope slide surface in seconds to confine small reagent volumes with samples and…
Hybridization and incubation Seals ad Chambers from Grace Bio-Labs are ideally suited for in situ-hybridization assays. The adhesive seal of…
In-situ hybridization ; Microarrays; Fluorescence In situ Hybridization (FISH); FRET (Fluorescence Resonance Energy Transfer);
FastWells™ are sticky, flexible silicone gaskets that form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Gaskets may…
FlexWell™ incubation chamber silicone gaskets form wells on slides using clean release adhesive to isolate up to 16 specimens per…
HybriSlips™ are rigid, light-weight, thin plastic coverslips that minimize friction and facilitate uniform reagent distribution during incubation steps which require…
ProPlates® were specifically designed to enable automated robotic liquid handling. Two main configurations are available: The ProPlate® Microtiter Plate is comprised…
Proteomics; Protein Microarrays; Protein expression analysis; Antibody profiling ; cDNA and oligonucleotide arrays;
Silicone isolator™ sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. Where additional sealing is…
Protein and DNA arrays ; Immunohistochemistry; Fluorescence In situ Hybridization (FISH) ; Biopolymers and hydrogel formulation ; Cryogenic-transmission electron microscopy (Cryo-TEM) ; Microwave crystallization ; Ultra-small-angle X-ray scattering (USAXS) ; Tissue ingeneering; Live cell lithography” (LCL);
Grace Bio-Labs microscopy reagents are manufactured in ISO-certified facilities to ensure the highest quality and consistency.
Imaging spacers are ultra-thin adhesive spacers which peel-and-stick to coverglass or microscope slides to confine specimens without compression. Layer multiple…
Imaging; Microscopy; High-temperature single-molecule kinetic analysis; Anti‐Stokes Raman scattering microscopy;
CoverWell ™ imaging chambers are designed to stabilize and support thick and free-floating specimens for confocal microscopy and imaging applications.…
Confocal microscopy ; Imaging ; Tissue and Cell staining ; High Resolution Microscopy ; Live-cell imaging ;
CoverWell ™ perfusion press-to-seal covers form water-tight, multiwell cell incubation or cytochemistry chambers when pressed to coverslips or microscope slides.…
Single molecule spectroscopy ; Live-cell imaging ; Microscoscopy ;
FastWells™ are sticky, flexible silicone gaskets that form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Gaskets may…
Microscopy ; Fluorescence In situ Hybridization (FISH) ; Single-molecule fluorescence analysis ; Immunohistochemistry ;
MutliSlip™ inserts with 8 (18mm x 18mm) or 15 (12mm x 12mm) No. 1.5 German glass coverglass per insert are…
High resolution microscopy; Fluorescent imaging; Immunohistochemistry ; Cell Culture;
These adhesive sheets are made using the same SecureSeal™ adhesive as is used to make HybriWell™ and SecureSeal™ Incubation Chambers. Thin,…
Imaging ; Tissue and Cell staining ; High Resolution Microscopy;
SecureSlip™ Silicone Supported Coverglass is affixed to a thin microscopically transparent silicone base which secures it to culture vessels by…
Imaging seals and chambers from Grace Bio-Labs offer a selection of tools for cell/tissue staining for high quality results in…
Tissue and Cell staining; High Resolution Microscopy; Live-cell imaging;
Grace Bio-Labs microscopy reagents are manufactured in ISO-certified facilities to ensure the highest quality and consistency.
CultureWell- removable chamber slide allows the cultivation and analysis of cells directly on a borosilicate microscope slide.
Fluorescence microscopy ; Confocal microscopy ; Cell differentiation and transfection; Immunohistochemistry ; Immunofluorescence; Immunostaining; Tissue and cell staining ; Fluorescence Resonance Energy Transfer (FRET) Microscopy;
CultureWell™ chambered coverglass products consist of removable and reusable, non-cytotoxic silicone gaskets secured to number 1.5 German coverglass. Chambered coverglass…
Cell Culture ; Fluorescence applications ; In-situ hybridization ; Immunostaining ;
CS16 CultureWell™ removable chambered coverglass is a 16-well chambered coverglass cell culture vessel, with 2 x 8 format with standard…
Cell Culture; Fluorescence applications; In-situ hybridization; Immunostaining ;
Each CultureWell™ coverglass insert is comprised of four chambered coverglass, assembled in a disposable frame placed in a standard 86mm…
High resolution microscopy ; Fluorescent imaging ; Immunohistochemistry;
Gaskets are ideal for forming wells on glass microscope slides or in polystyrene dishes. Gaskets are non-sterile and may be…
Cell Culture; High resolution microscopy; Fluorescent imaging ; Immunohistochemistry;
CultureWell™ clear silicone sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. They may be…
Cell Culture; High resolution microscopy ; Fluorescent imaging; Immunohistochemistry;
MutliSlip™ inserts with 8 (18mm x 18mm) or 15 (12mm x 12mm) No. 1.5 German glass coverglass per insert are…
SecureSlip™ Silicone Supported Coverglass is affixed to a thin microscopically transparent silicone base which secures it to culture vessels by…
CultureWell™ NON Removable Chambered Coverglass, 16 Well, No. 1.5 German borosilicate Coverglass. Product consists of cell culture vessels, with a…
Cell Culture ; Fluorescence applications; Small volume incubation ; Immunostaining;
Wound splints are constructed of silicone and include suture sites for increased precision in affixing on or within an animal…
Silicone isolator™ sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. Where additional sealing is…
Protein and DNA arrays ; Immunohistochemistry ; Fluorescence In-situ Hybridization (FISH) ; Biopolymers and hydrogel formulation; Cryogenic-transmission electron microscopy (Cryo-TEM) ; X-ray scattering ; Microwave crystallization ; Ultra-small-angle X-ray scattering (USAXS) ; Tissue engineering Live cell lithography (LCL);
CultureWell™ clear silicone sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. They may be…
Lorem Ipsum ; Lorem Ipsum; Lorem Ipsum;
Near Infrared Detection with Porous Nitrocellulose Films Improves Sensitivity for Protein Arrays. Despite some clear performance advantages of porous nitrocellulose Film-Slides for protein arrays, one objection to their use is the inherent fluorescence background detected on most scanners when using popular organic dyes with visible emission wavelengths (500-650 nm). However, fluorescence detection in near infrared … Continued
Michael A. Shultz, Aki Ohdera, Jason MacManiman, Charles M. McGrath. Grace Bio-Labs, Inc. ABSTRACT Super G Blocking Buffer was developed to produce low backgrounds in assays using nitrocellulose film-slides. Using this blocking reagent in combination with ONCYTE® SuperNOVA Film-slides, we achieved an order of magnitude greater sensitivity in a cytokine microarray assay than with … Continued
Microarrays deposited onto solid supports are robust platforms for performing antibody capture and antigen detection assays. Among various substrates, thin film porous nitrocellulose cast on glass surfaces has excelled in the field of proteomics since its invention by scientists at Grace Bio-Labs (McGrath, CM, Grudzien, J and A. Levine. Cell Vision 2:499-509, 1995). The irregular 3-dimensional … Continued
Protein microarray technology has progressed significantly and, although new microarray surface chemistries continue to be developed and marketed, porous nitrocellulose has remained the predominant immobilization surface for protein microarray applications due to its many functional advantages. The 3-dimensional structure of nitrocellulose film slides offers a considerably higher surface area for protein binding compared to conventional … Continued
Microarray results are susceptible to variation introduced from multiple sources during the course of assay methodologies employed with this technology. A commonly overlooked parameter which may significantly impact results is the choice of chamber/vessel for performing microarray incubations and washes. Chambers which allow for active mixing can significantly improve microarray data. Grace Bio-Labs offers ProPlate® incubation chambers which allow for continuous mixing during microarray assays … Continued
Fluorescence detection is a preferred method for many quantitative protein microarray assays in research and diagnostic applications due to its excellent sensitivity and potential for multiplexing. Use of multiplexed fluorescence-based assays on porous nitrocellulose though has been hampered by high backgrounds at shorter excitation wavelengths (such as the 532 nm fluorescence channel). We have developed … Continued
Multiplexed protein arrays offer significant advantages for diagnostics over monoplex assays such as ELISA’s. For multiplex detection, fluorescence is the label of choice, offering rapid and sensitive detection and large dynamic range in addition to the ability to separate signals from multiple fluors.While porous nitrocellulose (NC) film slides are an excellent substrate for protein arrays … Continued
Previously, we have reported data from multiplexed protein microarray assays with quantum dots which show great promise for use of these labels with nitrocellulose film-slides. The use of quantum dots with emission in the red and NIR spectrum allow for sensitive detection on nitrocellulose film slides due to low inherent fluorescence background at longer wavelengths.There … Continued
