Tag: Selection

Optimizing Reverse Phase Protein Array Assays

Components of Reverse-Phase Protein Array (RPPA) methods and their impact on the overall performance of this assay were presented at the 2nd annual RPPA Conference in Edinburgh, Scotland on November 12.  Entire presentation can be viewed here.  View poster here. View here for information regarding our RPPA Assay System Selecting Surfaces, Reagents, and Detection Methods … Continued

From Western Blot to Protein Array with Super G Blocking Buffer

Results obtained from Western blots can be highly variable and dependent not only on the specific antigen/antibody pairs used, but also on other reagents in the protocol such as blocking buffer. Not surprisingly, no one “universal” blocking reagent works well with all proteins. Thus choice of blocking reagent should be assessed for optimal results and having … Continued

Optimize Your Protein Array with Specially Formulated Arraying Buffer

The buffer used for spotting proteins for protein arrays is a critical factor in obtaining reliable and consistent results. Most important impact is on protein aggregation, which can affect spot morphology,  and protein binding to the substrate. Grace Bio-Labs has developed a Protein Arraying Buffer designed to work with our ONCYTE® porous nitrocellulose film slides for optimal … Continued