In a recent paper published in the Journal of Neuroinflammation the immune response associated with elevated intermittent ocular hypertension has been evaluated. An antigen microarray assay was performed with ten different antigens spotted on ONCYTE® nitrocellulose film slides and used to probe serum from an intermittent ocular hypertension animal model. High Intraocular pressure is a main risk … Continued
Grace Bio-Labs will be attending the 6th Global RPPA Meeting in Reutlingen/Germany. Come meet our representatives!
Introduction Profiling sets of multiple protein markers and signaling pathways is becoming the standard method for biomarker discovery, accurate disease diagnosis, and monitoring of therapeutic response. Low-cost, high throughput multiplexed assay platforms that can provide specific, sensitive, and reproducible measurements, featuring reliable and robust assay controls along with easy to use methodologies will yield increased … Continued
Application of Reverse-Phase Protein Arrays (RPPA) has primarily centered on the discovery and identification of cell signaling pathways involved in cancer development and progression. The RPPA approach and technology has found expanded utility in other disciplines such as in the study of inflammatory diseases.Recently Negm, et al. employed RPPA to study the signaling pathways downstream … Continued
ONCYTE porous nitrocellulose (PNC) film slides are a 3-D substrate very well suited for protein microarrays due to its high binding capacity and ability to retain proteins’ conformation and antigenic sites. PNC slides have proven extremely valuable for Reverse-Phase Protein Arrays (RPPAs) in the field of cancer research.In recent years, interest in further improving the … Continued
Reverse phase protein arrays (RPPA) are designed for sensitive quantitation of a target protein in a very small sample of tissue lysate, and multiplex analysis of many samples in one experiment. This technology holds great promise for the identification and analysis of critical biomarkers for disease states in patient samples and for high-throughput drug screening … Continued
A SYSTEMS APPROACH TO QUANTITATIVE MULTIPLEXED RPPA’S Presented by: Michael A. Shultz, PhD RPPA Workshop in Kobe, Japan November, 2013 Porous nitrocellulose film (PNC) is the substrate of choice for RPPA largely because of its extraordinarily high protein binding capacity, which can exceed non-porous planar counterparts by 500X.
Components of Reverse-Phase Protein Array (RPPA) methods and their impact on the overall performance of this assay were presented at the 2nd annual RPPA Conference in Edinburgh, Scotland on November 12. Entire presentation can be viewed here. View poster here. View here for information regarding our RPPA Assay System Selecting Surfaces, Reagents, and Detection Methods … Continued
Reverse phase protein arrays (RPPA) offer great promise for the identification and analysis of critical biomarkers for disease states in patient samples and for high-throughput drug screening using cultured cell systems. Porous nitrocellulose (NC) film slides continue to be the preferred substrate for RPPA due to their high protein binding capacity and many functional advantages. … Continued
Multiplexed protein arrays offer significant advantages for diagnostics over monoplex assays such as ELISA’s. For multiplex detection, fluorescence is the label of choice, offering rapid and sensitive detection and large dynamic range in addition to the ability to separate signals from multiple fluors.While porous nitrocellulose (NC) film slides are an excellent substrate for protein arrays … Continued
Reverse phase protein arrays (RPPA) offer great promise for the identification and use of critical biomarkers for drug screening and diagnostics. Samples prepared from tissue lysate that are spotted for RPPA can often produce dilute protein concentrations, sometimes only a few hundred µg/ml. For single spot depositions in an array, this utilizes less than 1% of … Continued
Building on the well-established ability of nitrocellulose to bind macromolecules in gel transfer applications, Grace Bio-Labs invented a method to cast thin porous nitrocellulose films directly onto glass microscope slides. These “Film-Slides” or “Films” were initially designed for a new platform for immunocytochemistry, called Cytocoherent Transfer (1, 2). Since that time, Film-Slides have become an established surface … Continued