Tag: Protein microarray

RPPA Analysis of Mild Traumatic Brain Injuries Biomarkers

A growing number of studies indicate that repeated mild traumatic brain injuries (RmTBI) are associated with significant and lasting neurological alterations and an increased risk of developing neurodegenerative diseases. Advanced Magnetic Resonance Imaging and blood-based protein analysis not only can provide a reliable method for the clinical assessment and treatment of RmTBI but also valuable … Continued

Malaria vaccine development: a microarray to identify new target proteins

Malaria is a life-threatening disease caused by Plasmodium parasites transmitted to people through the bites of infected female Anopheles mosquitoes. In 2017, it was estimated that nearly half of the world population was at risk of contracting the disease, with the highest number of cases and deaths reported in sub-Saharan Africa. In the same year … Continued

Epitope Mapping Using Peptide Microarray

Epitope mapping allows the identification of polyclonal antibodies produced as a response to cytotoxins, allergens, neuronal or inflammatory conditions and, in autoimmune diseases to autoantigens. Routine assays for autoimmune response profiling are generally performed by ELISAs and fluorescence immunoassays. Individual assays are performed in microtiter plates, with each well representing a single antigen. Peptide microarrays provide … Continued

Applications of Protein Microarrays for Biomarker Discovery.

In a recent paper published in the Journal of Neuroinflammation the immune response associated with elevated intermittent ocular hypertension has been evaluated.  An antigen microarray assay was performed with ten different antigens spotted on ONCYTE®  nitrocellulose film slides and used to probe serum from an intermittent ocular hypertension animal model. High Intraocular pressure is a main risk … Continued

Multiplexed ONCYTE Protein microarrays Used for Infectious Disease Immunogen Discovery

First, in an interesting Shistotosomiasis study, Gaze, et al, employ protein microarrays in a longitudinal immunomics study for vaccine antigen discovery (1).  Schistosomiasis is a pervasive disease affecting 200 million people a year and traditional drug treatments are ineffective due to re-infection, drug expense, and parasite drug resistance. Non-sterile immunity does develop over long periods of … Continued

A Comprehensive Technology Survey of Protein Microarray Platforms, Techniques and Detection Methods

A comprehensive assessment of multiplex immunoassay platforms and detection methods is summarized in an interesting 2011 article by Chandra, et al, in Expert Review Proteomics (1). The article, “Protein Microarrays and Novel Detection Platforms” provides interesting information for several reasons.First, it presents an excellent description of the emerging discovery and clinical need for high throughput … Continued

Sensitive and Accurate Multi-Color RPPA Using Grace Bio-Labs ArrayCAM Protein Microarray System

Introduction Profiling sets of multiple protein markers and signaling pathways is becoming the standard method for biomarker discovery, accurate disease diagnosis, and monitoring of therapeutic response. Low-cost, high throughput multiplexed assay platforms that can provide specific, sensitive, and reproducible measurements, featuring reliable and robust assay controls along with easy to use methodologies will yield increased … Continued

Targeted Malarial Serological Diagnostics using ONCYTE Porous Nitrocellulose Film Microarray

Targeted diagnostic serological screening using ONCYTE porous nitrocellulose (PNC) microarrays is currently greatly expanding. Interesting research in the Felgner laboratory at UC Irvine has centered on identifying and monitoring P. falciparum antigens involved in malaria infection using subsets of its viral proteome produced via a recombinant expression system and arraying on PNC. Recently, in collaboration … Continued

Quantum Nanocrystal (QNC) Detection and ONCYTE AVID Porous Nitrocellulose Results in Improved RPPA Quantitation and Dynamic Range

ONCYTE porous nitrocellulose (PNC) film slides are a 3-D substrate very well suited for protein microarrays due to its high binding capacity and ability to retain proteins’ conformation and antigenic sites. PNC slides have proven extremely valuable for Reverse-Phase Protein Arrays (RPPAs) in the field of cancer research.In recent years, interest in further improving the … Continued

Can Vaccine Development Be Accelerated by Protein Array Analysis?

New Finding for Malaria Vaccine Shows Promise. Vaccine development has been a key strategy for fighting parasitic and viral diseases such as polio, hepatitis, measles, smallpox, and tuberculosis. Despite the advances in molecular biology, the rate of vaccine development has surprisingly not accelerated over the past century, and many diseases that affect world health remain … Continued

U1 snRNP and Smith Antigens in Autoimmune Diagnostics

We are pleased to present a recent talk by Grace Bio-Labs Scientist Kevin Carman.       Summary: U1 snRNP and Smith antigen auto-antibodies are important markers in several autoimmune disorders. Auto-antibodies against these extractable nuclear antigens (ENA’s) are commonly used as clinical diagnostic criteria for Sjorgen’s Syndrome, SLE, and MCTD.