Tag: Protein binding

Multiplex Microarrays: The Next Generation ELISA

The hallmark of chronic diseases is the production of highly specific autoantibodies (1,2).  These autoantibodies are increasingly measured to guide clinical decision making.    The goal of these measurements is to define intracellular signal pathway activation profiles. These profiles can be integrated with clinical information to generate “biosignatures” for individual patients, the goal of which is personalized … Continued

View Your Proteins in 3-D!

Protein microarray technology has progressed significantly and, although new microarray surface chemistries continue to be developed and marketed, porous nitrocellulose has remained the predominant immobilization surface for protein microarray applications due to its many functional advantages.  The 3-dimensional structure of nitrocellulose film slides offers a considerably higher surface area for protein binding compared to conventional … Continued

Print Macroarrays with ProPlate

ProPlate® Chambers for Macroarray Printing Many laboratories desire the benefits of utilizing microarray technology in their field of interest but do not have access to microarray printing equipment or cannot justify the expense of dedicated print runs for lower throughput experiments.  In many cases where highly multiplexed assays are not a necessity or during early-phase … Continued

Optimize Your Protein Array with Specially Formulated Arraying Buffer

The buffer used for spotting proteins for protein arrays is a critical factor in obtaining reliable and consistent results. Most important impact is on protein aggregation, which can affect spot morphology,  and protein binding to the substrate. Grace Bio-Labs has developed a Protein Arraying Buffer designed to work with our ONCYTE® porous nitrocellulose film slides for optimal … Continued