Protein Crystallization

Protein crystallization is a key assay for structural studies of proteins. The protocols for crystallization of protein are challenging due to the stringent requirement for pure samples and control of environmental conditions during the crystallization process. Vapor diffusion using hanging drop is a preferred method for obtaining quality crystals with…

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Buffers and Reagents

High-quality, ready-to-use buffers and reagents manufactured in ISO- certified facilities.

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Grace Bio-Labs nitrocellulose microarray substrates
forward phase protein microarray
Reverse phase protein array

Protein Microarray Substrates

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Grace Bio-Labs microarray surface chemistry is based on the well-known protein-binding properties of nitrocellulose. A range of different formulations have…

APPLICATIONS:

Antigen-Capture AssayAntibody Capture AssayCoronavirus Antigen ArrayEpitope-mappingBiomarker Discovery and ValidationImmunogen Discovery

DNA Microarray Substrates

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Epoxy Microarray Slides provide a uniform substrate for a variety of DNA/RNA-based diagnostic applications.

APPLICATIONS:

DNA/Oligonucleotide Microarray ;  microRNA Microarray ;  Single Nucleotide Polymorphism (SNP) Analysis ;  Gene Expression Profiling; 

Microarray Reagents

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Grace Bio-Labs microarray regents have been specifically formulated to achieve the full potential of porous nitrocellulose, accelerating experimental design and…

APPLICATIONS:

Antigen-Capture Assay Antibody Capture Assay RPPA- Reverse Phase Protein Microarray Laser micro-dissection RRPA Epitope-mapping Biomarker Discovery and Validation Immunogen Discovery

NanoParticle Fluorescent Calibration Slide

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Photostable nanoparticles arrayed on glass slides for calibration of fluorescence imaging systems and quantitative analysis.

APPLICATIONS:

Calibration of Microarray Scanners ;  Quantitative Microarray Analyses Microscope Focal Plane Adjustment;  Microscope Focal Plane Adjustment; 

ProPlates® Multi-Well Chambers

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ProPlate® re-usable, multi-well chambers are available in a wide variety of formats to fit standard 1 x 25 x 75…

APPLICATIONS:

Microarray ;  Hybridization and Incubation;  ELISA;  PCR and NGS; 

CoverWell™ Incubation Chambers

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CoverWell™ incubation chambers are reusable, easy to apply chambers that attach without the use of adhesive.  CoverWells™ enclose a large…

APPLICATIONS:

Reverse Transfection Microarray;  DNA Microarray;  In-situ hybridization;  Immunohistochemistry; 

Silicone Isolators

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Silicone Isolators allow researchers to isolate specimens using removable hydrophobic barriers. They may be used to isolate cells grown in…

SecureSeal™ Hybridization Chambers

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SecureSeal™ Hybridization Chambers are thin, silicone-gasketed chambers providing optimal surface-to-volume fluid dynamics for hybridization assays on large or multiple specimens…

HybriWell™ Sealing System

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HybriWell™ Sealing System bonds securely to a microscope slide surface in seconds to confine small reagent volumes with samples and…

Hybridization and Incubation

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Hybridization and incubation Seals ad Chambers from Grace Bio-Labs are ideally suited for in situ-hybridization assays. The adhesive seal of…

APPLICATIONS:

In-situ hybridization MicroarraysFluorescence In situ Hybridization (FISH)FRET (Fluorescence Resonance Energy Transfer)

FastWells™ Reagent Barriers

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FastWells™ are sticky, flexible silicone gaskets that form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Gaskets may…

FlexWell™ Incubation Chambers

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FlexWell™ incubation chamber silicone gaskets form wells on slides using clean release adhesive to isolate up to 16 specimens per…

APPLICATIONS:

Protein MicroarrayHybridizationIncubation

HybriSlip™ Hybridization Covers

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HybriSlips™ are rigid, light-weight, thin plastic coverslips that minimize friction and facilitate uniform reagent distribution during incubation steps which require…

ProPlates®

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ProPlates® were specifically designed to enable automated robotic liquid handling. Two main configurations are available: The ProPlate® Microtiter Plate is comprised…

APPLICATIONS:

ProteomicsProtein Microarrays;  Protein expression analysis;  Antibody profiling cDNA and oligonucleotide arrays

Silicone Isolators™ Sheet Material

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Silicone isolator™ sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. Where additional sealing is…

APPLICATIONS:

Protein and DNA arrays ;  Immunohistochemistry;  Fluorescence In situ Hybridization (FISH) ;  Biopolymers and hydrogel formulation ;  Cryogenic-transmission electron microscopy (Cryo-TEM) ;  Microwave crystallization ;  Ultra-small-angle X-ray scattering (USAXS) ;  Tissue ingeneering;  Live cell lithography” (LCL); 

CultureWell Removable chamber slide 8 well Catalog #: 103542

Microscopy Reagents

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Grace Bio-Labs microscopy reagents are manufactured in ISO-certified facilities to ensure the highest quality and consistency.

Imaging Spacers

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Imaging spacers are ultra-thin adhesive spacers which peel-and-stick to coverglass or microscope slides to confine specimens without compression. Layer multiple…

APPLICATIONS:

Imaging;  Microscopy;  High-temperature single-molecule kinetic analysis;  Anti‐Stokes Raman scattering microscopy; 

CoverWell™ Imaging Chambers

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CoverWell ™ imaging chambers are designed to stabilize and support thick and free-floating specimens for confocal microscopy and imaging applications.…

APPLICATIONS:

Confocal microscopy Imaging Tissue and Cell staining ;  High Resolution Microscopy ;  Live-cell imaging ; 

CoverWell™ Perfusion Chambers

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CoverWell ™ perfusion press-to-seal covers form water-tight, multiwell cell incubation or cytochemistry chambers when pressed to coverslips or microscope slides.…

APPLICATIONS:

Single molecule spectroscopy Live-cell imaging Microscoscopy

FastWells™ Reagent Barriers

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FastWells™ are sticky, flexible silicone gaskets that form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Gaskets may…

APPLICATIONS:

Microscopy Fluorescence In situ Hybridization (FISH) Single-molecule fluorescence analysis ;  Immunohistochemistry ; 

MultiSlip™ Coverglass Inserts

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MutliSlip™ inserts with 8 (18mm x 18mm) or 15 (12mm x 12mm) No. 1.5 German glass coverglass per insert are…

APPLICATIONS:

High resolution microscopy Fluorescent imaging Immunohistochemistry ;  Cell Culture; 

SecureSeal™ Adhesive Sheets

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These adhesive sheets are made using the same SecureSeal™ adhesive as is used to make HybriWell™ and SecureSeal™ Incubation Chambers.  Thin,…

APPLICATIONS:

Imaging ;  Tissue and Cell staining ;  High Resolution Microscopy; 

SecureSlip™ Silicone Supported Coverglass

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SecureSlip™ Silicone Supported Coverglass is affixed to a thin microscopically transparent silicone base which secures it to culture vessels by…

Imaging and Microscopy

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Imaging seals and chambers from Grace Bio-Labs offer a selection of tools for cell/tissue staining for high quality results in…

APPLICATIONS:

Tissue and Cell stainingHigh Resolution MicroscopyLive-cell imaging

CultureWell Removable chamber slide 8 well Catalog #: 103542

Cell Culture Reagents

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Grace Bio-Labs microscopy reagents are manufactured in ISO-certified facilities to ensure the highest quality and consistency.

CultureWell removable chamber slide

CultureWell™ Removable Chamber Slide

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CultureWell- removable chamber slide allows the cultivation and analysis of cells directly on a borosilicate microscope slide.

APPLICATIONS:

Fluorescence microscopy ;  Confocal microscopy ;  Cell differentiation and transfection;  Immunohistochemistry ;  Immunofluorescence;  Immunostaining;  Tissue and cell staining ;  Fluorescence Resonance Energy Transfer (FRET) Microscopy; 

CultureWell™ MultiWell Chambered Coverslips

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CultureWell™ chambered coverglass products consist of removable and reusable, non-cytotoxic silicone gaskets secured to number 1.5 German coverglass. Chambered coverglass…

APPLICATIONS:

Cell Culture Fluorescence applications In-situ hybridization Immunostaining

CS16-CultureWell™ Removable Chambered Coverglass

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CS16 CultureWell™ removable chambered coverglass is a 16-well chambered coverglass cell culture vessel, with 2 x 8 format with standard…

APPLICATIONS:

Cell CultureFluorescence applicationsIn-situ hybridizationImmunostaining

CultureWell™ Coverglass Inserts

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Each CultureWell™ coverglass insert is comprised of four chambered coverglass, assembled in a disposable frame placed in a standard 86mm…

APPLICATIONS:

High resolution microscopy Fluorescent imaging Immunohistochemistry

CultureWell™ Reusable Gaskets

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Gaskets are ideal for forming wells on glass microscope slides or in polystyrene dishes. Gaskets are non-sterile and may be…

APPLICATIONS:

Cell CultureHigh resolution microscopyFluorescent imaging Immunohistochemistry

CultureWell™ Silicone Sheet Material

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CultureWell™ clear silicone sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. They may be…

APPLICATIONS:

Cell CultureHigh resolution microscopy Fluorescent imagingImmunohistochemistry

MultiSlip™ Coverglass Inserts

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MutliSlip™ inserts with 8 (18mm x 18mm) or 15 (12mm x 12mm) No. 1.5 German glass coverglass per insert are…

APPLICATIONS:

Cell CultureFluorescent imaging Immunohistochemistry

SecureSlip™ Silicone Supported Coverglass

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SecureSlip™ Silicone Supported Coverglass is affixed to a thin microscopically transparent silicone base which secures it to culture vessels by…

APPLICATIONS:

Cell CultureImmunofluorescence assayMicroscopy

CultureWell™ ChamberSLIP 16, Non-Removable Chambered Coverglass

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CultureWell™ NON Removable Chambered Coverglass, 16 Well, No. 1.5 German borosilicate Coverglass. Product consists of cell culture vessels, with a…

APPLICATIONS:

Cell Culture Fluorescence applicationsSmall volume incubation Immunostaining

Silicone Wound Splints

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Wound splints are constructed of silicone and include suture sites for increased precision in affixing on or within an animal…

Silicone Isolator Sheet Material

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Silicone isolator™ sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. Where additional sealing is…

APPLICATIONS:

Protein and DNA arrays ;  Immunohistochemistry ;  Fluorescence In-situ Hybridization (FISH) ;  Biopolymers and hydrogel formulation;  Cryogenic-transmission electron microscopy (Cryo-TEM) ;  X-ray scattering ;  Microwave crystallization ;  Ultra-small-angle X-ray scattering (USAXS) ;  Tissue engineering Live cell lithography (LCL); 

CultureWell Silicone Sheet Material

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CultureWell™ clear silicone sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. They may be…

APPLICATIONS:

Lorem Ipsum ;  Lorem Ipsum;  Lorem Ipsum; 

Tag: ONCYTE

Multiplexed ONCYTE Protein microarrays Used for Infectious Disease Immunogen Discovery

First, in an interesting Shistotosomiasis study, Gaze, et al, employ protein microarrays in a longitudinal immunomics study for vaccine antigen discovery (1).  Schistosomiasis is a pervasive disease affecting 200 million people a year and traditional drug treatments are ineffective due to re-infection, drug expense, and parasite drug resistance. Non-sterile immunity does develop over long periods of … Continued

Improved Classification of Influenza Infection, Future Susceptibility and Pandemic Epidmiology using ONCYTE Porous Nitrocellulose Film Microarrays

An interesting paper furthering the expansion of infectious disease serological applications using ONCYTE porous nitrocellulose film slides was described by researchers out of the Netherlands for classifying influenza infections and susceptibility to future infections. Microarrays printed on ONCYTE AVID film slides used purified recombinant influenza virus hemagglutinin antigens (HA1) from various subtypes (H1, H2, H3, … Continued

Targeted Malarial Serological Diagnostics using ONCYTE Porous Nitrocellulose Film Microarray

Targeted diagnostic serological screening using ONCYTE porous nitrocellulose (PNC) microarrays is currently greatly expanding. Interesting research in the Felgner laboratory at UC Irvine has centered on identifying and monitoring P. falciparum antigens involved in malaria infection using subsets of its viral proteome produced via a recombinant expression system and arraying on PNC. Recently, in collaboration … Continued

Quantum Nanocrystal (QNC) Detection and ONCYTE AVID Porous Nitrocellulose Results in Improved RPPA Quantitation and Dynamic Range

ONCYTE porous nitrocellulose (PNC) film slides are a 3-D substrate very well suited for protein microarrays due to its high binding capacity and ability to retain proteins’ conformation and antigenic sites. PNC slides have proven extremely valuable for Reverse-Phase Protein Arrays (RPPAs) in the field of cancer research.In recent years, interest in further improving the … Continued

Review of Mujawar et al Analytical Chemistry – Distribution of Biomolecules in Porous Nitrocellulose Films

An interesting paper in (Analytical Chemistry) describes the use of confocal fluorescence section imaging to analyze protein distribution in microarray spots on nitrocellulose film slides. By scanning and imaging through the Z dimension of a printed spot of fluor labeled protein, a 3-dimensional quantitation of fluorophore density in the films was obtained, showing that commercial … Continued

Multiplex Microarrays: The Next Generation ELISA

The hallmark of chronic diseases is the production of highly specific autoantibodies (1,2).  These autoantibodies are increasingly measured to guide clinical decision making.    The goal of these measurements is to define intracellular signal pathway activation profiles. These profiles can be integrated with clinical information to generate “biosignatures” for individual patients, the goal of which is personalized … Continued

Sensitive Detection of Total Protein on RPPA using Fast Green

Reverse phase protein arrays (RPPA) are designed for sensitive quantitation of a target protein in a very small sample of tissue lysate, and multiplex analysis of many samples in one experiment. This technology holds great promise for the identification and analysis of critical biomarkers for disease states in patient samples and for high-throughput drug screening … Continued

High Capacity Porous Nitrocellulose Microarray Substrates Enable Whole Proteome Serology Screening Assays for Antigenicity Profiling of Infectious Disease Agents

Insight into the dynamics of pathogenic infectivity has been greatly enhanced by studies using whole proteome microarray technology [1]. Techniques allowing high throughput production of recombinant proteins representing comprehensive antigenic potential of pathogenic bacteria and viruses allow examination of the degrees to which specific antigenic proteins are involved in human serological response.The true value of … Continued

Beating Background Fluorescence on Nitrocellulose Film-Slides

Near Infrared Detection with Porous Nitrocellulose Films Improves Sensitivity for Protein Arrays. Despite some clear performance advantages of porous nitrocellulose Film-Slides for protein arrays, one objection to their use is the inherent fluorescence background detected on most scanners when using popular organic dyes with visible emission wavelengths (500-650 nm). However, fluorescence detection in near infrared … Continued

Optimized Blocking Of Porous Nitrocellulose Films For Sensitive Protein Microarrays

Michael A. Shultz, Aki Ohdera, Jason MacManiman, Charles M. McGrath. Grace Bio-Labs, Inc.   ABSTRACT Super G Blocking Buffer was developed to produce low backgrounds in assays using nitrocellulose film-slides.  Using this blocking reagent in combination with ONCYTE® SuperNOVA Film-slides, we achieved an order of magnitude greater sensitivity in a cytokine microarray assay than with … Continued

Inclusion of Super G in All Steps of Protein Arrays Improves S/N

Blocking reagents are used to reduce non-specific protein binding in immunoassays, significantly improving results in terms of Signal-to-Noise ratio. Typically the blocking reagent is added prior to addition of the primary detection antibody.In this study we asked the question as to whether addition of the blocking reagent to other steps in a protein array assay … Continued

Preserving Protein Immuno-Reactivity

Microarrays deposited onto solid supports are robust platforms for performing antibody capture and antigen detection assays.  Among various substrates, thin film porous nitrocellulose cast on glass surfaces has excelled in the field of proteomics since its invention by scientists at Grace Bio-Labs (McGrath, CM, Grudzien, J and A. Levine. Cell Vision 2:499-509, 1995). The irregular 3-dimensional … Continued