Tag: Microarray

Improved Classification of Influenza Infection, Future Susceptibility and Pandemic Epidmiology using ONCYTE Porous Nitrocellulose Film Microarrays

An interesting paper furthering the expansion of infectious disease serological applications using ONCYTE porous nitrocellulose film slides was described by researchers out of the Netherlands for classifying influenza infections and susceptibility to future infections. Microarrays printed on ONCYTE AVID film slides used purified recombinant influenza virus hemagglutinin antigens (HA1) from various subtypes (H1, H2, H3, … Continued

Multiplex Microarrays: The Next Generation ELISA

The hallmark of chronic diseases is the production of highly specific autoantibodies (1,2).  These autoantibodies are increasingly measured to guide clinical decision making.    The goal of these measurements is to define intracellular signal pathway activation profiles. These profiles can be integrated with clinical information to generate “biosignatures” for individual patients, the goal of which is personalized … Continued

From Western Blot to Protein Array with Super G Blocking Buffer

Results obtained from Western blots can be highly variable and dependent not only on the specific antigen/antibody pairs used, but also on other reagents in the protocol such as blocking buffer. Not surprisingly, no one “universal” blocking reagent works well with all proteins. Thus choice of blocking reagent should be assessed for optimal results and having … Continued

ProPlate® Chambers Minimize Microarray Variation

Microarray results are susceptible to variation introduced from multiple sources during the course of assay methodologies employed with this technology. A commonly overlooked parameter which may significantly impact results is the choice of chamber/vessel for performing microarray incubations and washes. Chambers which allow for active mixing can significantly improve microarray data. Grace Bio-Labs offers ProPlate® incubation chambers which allow for continuous mixing during microarray assays … Continued

Protein Microarrays: Investment Required

As scientists we are trained to be independent thinkers, and to value the expertise gained on the learning curve of new technologies. When it comes to microarrays, however, outsourcing or collaborating with an expert can accelerate the development process.“There is a serious investment required in assay development for microarrays,” says Stacey Clarken, VP of Commercial … Continued