Tag: Increase sensitivity

View Your Proteins in 3-D!

Protein microarray technology has progressed significantly and, although new microarray surface chemistries continue to be developed and marketed, porous nitrocellulose has remained the predominant immobilization surface for protein microarray applications due to its many functional advantages.  The 3-dimensional structure of nitrocellulose film slides offers a considerably higher surface area for protein binding compared to conventional … Continued

ProPlate® Chambers Minimize Microarray Variation

Microarray results are susceptible to variation introduced from multiple sources during the course of assay methodologies employed with this technology. A commonly overlooked parameter which may significantly impact results is the choice of chamber/vessel for performing microarray incubations and washes. Chambers which allow for active mixing can significantly improve microarray data. Grace Bio-Labs offers ProPlate® incubation chambers which allow for continuous mixing during microarray assays … Continued

SuperNOVA: Superior Porous Nitrocellulose Surface for Fluorescence Detection

Fluorescence detection is a preferred method for many quantitative protein microarray assays in research and diagnostic applications due to its excellent sensitivity and potential for multiplexing.  Use of multiplexed fluorescence-based assays on porous nitrocellulose though has been hampered by high backgrounds at shorter excitation wavelengths (such as the 532 nm fluorescence channel). We have developed … Continued