Tag: Grace Bio-Labs

Epitope Mapping Using Peptide Microarray

Epitope mapping allows the identification of polyclonal antibodies produced as a response to cytotoxins, allergens, neuronal or inflammatory conditions and, in autoimmune diseases to autoantigens. Routine assays for autoimmune response profiling are generally performed by ELISAs and fluorescence immunoassays. Individual assays are performed in microtiter plates, with each well representing a single antigen. Peptide microarrays provide … Continued

Applications of Protein Microarrays for Biomarker Discovery.

In a recent paper published in the Journal of Neuroinflammation the immune response associated with elevated intermittent ocular hypertension has been evaluated.  An antigen microarray assay was performed with ten different antigens spotted on ONCYTE®  nitrocellulose film slides and used to probe serum from an intermittent ocular hypertension animal model. High Intraocular pressure is a main risk … Continued

Grace Bio-Labs Featured at AACR 2016

Grace Bio-Labs will be featured at the 2016 American Association for Cancer Research (AACR) annual meeting, April 16-20 in New Orleans.  Our products are highlighted in a presentation by scientists at George Mason University, titled “Chloroquine and Vitamin D3 Modulate Proliferation in Early Stage Breast Cancer Models.” To view the full abstract, click here.  Representatives … Continued

Sensitive and Accurate Multi-Color RPPA Using Grace Bio-Labs ArrayCAM Protein Microarray System

Introduction Profiling sets of multiple protein markers and signaling pathways is becoming the standard method for biomarker discovery, accurate disease diagnosis, and monitoring of therapeutic response. Low-cost, high throughput multiplexed assay platforms that can provide specific, sensitive, and reproducible measurements, featuring reliable and robust assay controls along with easy to use methodologies will yield increased … Continued

Validation of a Novel One-Step Tissue Fixation Chemistry That Preserves Phosphoproteins and Histomorphology

  George Mason University Poster Presentation for the National Cancer Institute’s 15th Annual IMAT Principle Investigators Meeting, November 13-15, 2014. Unmet need: A significant and underappreciated issue is the fact that excised tissue is alive and reacting to ex vivo stress [1]. During this “cold ischemia time” cells within the tissue react and adapt to the absence … Continued

Enabling Cell-based Assays and Translation to Personalized Medicine

Cell-based assays have expanded in use, and reduced in sample requirements over the past decade, driven by technologies that have improved the culture and monitoring of cell functions on a miniature scale. One of our partners, Microstem Inc., based in San Diego, CA, is an example of an early stage reagent development company that approached … Continued

Sensitive Detection of Total Protein on RPPA using Fast Green

Reverse phase protein arrays (RPPA) are designed for sensitive quantitation of a target protein in a very small sample of tissue lysate, and multiplex analysis of many samples in one experiment. This technology holds great promise for the identification and analysis of critical biomarkers for disease states in patient samples and for high-throughput drug screening … Continued

A Systems Approach to Quantitative Multiplexed RPPA

A SYSTEMS APPROACH TO QUANTITATIVE MULTIPLEXED RPPA’S Presented by: Michael A. Shultz, PhD  RPPA Workshop in Kobe, Japan November, 2013 Porous nitrocellulose film (PNC) is the substrate of choice for RPPA largely because of its extraordinarily high protein binding capacity, which can exceed non-porous planar counterparts by 500X.

Beating Background Fluorescence on Nitrocellulose Film-Slides

Near Infrared Detection with Porous Nitrocellulose Films Improves Sensitivity for Protein Arrays. Despite some clear performance advantages of porous nitrocellulose Film-Slides for protein arrays, one objection to their use is the inherent fluorescence background detected on most scanners when using popular organic dyes with visible emission wavelengths (500-650 nm). However, fluorescence detection in near infrared … Continued

Optimized Blocking Of Porous Nitrocellulose Films For Sensitive Protein Microarrays

Michael A. Shultz, Aki Ohdera, Jason MacManiman, Charles M. McGrath. Grace Bio-Labs, Inc.   ABSTRACT Super G Blocking Buffer was developed to produce low backgrounds in assays using nitrocellulose film-slides.  Using this blocking reagent in combination with ONCYTE® SuperNOVA Film-slides, we achieved an order of magnitude greater sensitivity in a cytokine microarray assay than with … Continued

Inclusion of Super G in All Steps of Protein Arrays Improves S/N

Blocking reagents are used to reduce non-specific protein binding in immunoassays, significantly improving results in terms of Signal-to-Noise ratio. Typically the blocking reagent is added prior to addition of the primary detection antibody.In this study we asked the question as to whether addition of the blocking reagent to other steps in a protein array assay … Continued