Tag: ELISA

Routine Clinical Adoption of Immunoassay Multiplexing, Whether Microarray or Cytometric Bead Array, Is Still Several Years Away Due to Operational Entrenchment

An objective look at immunoassay multiplexing, where it stands today and contrasted to the standard ELISA was authored recently by Dr. Lucy Fairclough et al. The discussion argued the efficacy of multiplexing as well as the pros and cons of present methods available.Commercial availability of multiplex immunoassays for life science discovery research is rapidly expanding … Continued

Multiplex Microarrays: The Next Generation ELISA

The hallmark of chronic diseases is the production of highly specific autoantibodies (1,2).  These autoantibodies are increasingly measured to guide clinical decision making.    The goal of these measurements is to define intracellular signal pathway activation profiles. These profiles can be integrated with clinical information to generate “biosignatures” for individual patients, the goal of which is personalized … Continued

Immunoassays: Protein Arrays vs. ELISA and Westerns

Studies of biological systems have expanded beyond the ‘one gene, one protein’ paradigm to the field of proteomics, or studying large numbers of proteins that act in a concert of complex biological systems. Thus in the post-genome era, technology has been driven to deliver multiplex assays that allow us to monitor multiple proteins in parallel, … Continued

Protein Microarrays: Investment Required

As scientists we are trained to be independent thinkers, and to value the expertise gained on the learning curve of new technologies. When it comes to microarrays, however, outsourcing or collaborating with an expert can accelerate the development process.“There is a serious investment required in assay development for microarrays,” says Stacey Clarken, VP of Commercial … Continued

Jumping the Hurdles for Microarray Diagnostics

A recent article (1)  reporting on a  conference  in Edinburgh, Scotland,  discusses the progress in ‘affinity proteomics’, that is, antibody-based assays including protein microarrays and their potential for use in clinical diagnostics. There continues to be much optimism around the use of antibody-based microarrays for diagnostics, and yetthe emergence of this technology has been slower than … Continued