Tag: Effective

From Western Blot to Protein Array with Super G Blocking Buffer

Results obtained from Western blots can be highly variable and dependent not only on the specific antigen/antibody pairs used, but also on other reagents in the protocol such as blocking buffer. Not surprisingly, no one “universal” blocking reagent works well with all proteins. Thus choice of blocking reagent should be assessed for optimal results and having … Continued

Super G Blocking Reagent for Western Blots

Although Super G blocking reagent was developed and optimized for fluorescent endpoint detection on nitrocellulose film slides, we have found that it is also an effective blocker for Western blot applications. Data presented below illustrate the effectiveness of Super G blocking reagent in typical Western blot experiments using nitrocellulose membranes by reducing background staining and … Continued