Tag: Confocal Microscopy

Confocal Fluorescence Microscopy of CRISPR-generated G6PD Knockout mouse liver cells (TIB-73)

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) Cas-9 knockout of glucose 6-phosphate dehydrogenase (G6PD) The first surviving G6PD- knockout somatic cell line was generated with CRISPR-Cas-9 technology to support subcellular localization studies. G6PD,  glucose 6-phosphate dehydrogenase, is the rate-limiting enzyme in the pentose phosphate pathway. G6PD is involved in oxidative stress, apoptosis, angiogenesis and cancer … Continued