Tag: Buffer

Optimized Blocking Of Porous Nitrocellulose Films For Sensitive Protein Microarrays

Michael A. Shultz, Aki Ohdera, Jason MacManiman, Charles M. McGrath. Grace Bio-Labs, Inc.   ABSTRACT Super G Blocking Buffer was developed to produce low backgrounds in assays using nitrocellulose film-slides.  Using this blocking reagent in combination with ONCYTE® SuperNOVA Film-slides, we achieved an order of magnitude greater sensitivity in a cytokine microarray assay than with … Continued

Protein Staining Nitrocellulose using SYPRO® Ruby

Reverse phase protein arrays (RPPA) offer great promise for the identification and analysis of critical biomarkers for disease states in patient samples and for high-throughput drug screening using cultured cell systems.  Porous nitrocellulose (NC) film slides continue to be the preferred substrate for RPPA due to their high protein binding capacity and many functional advantages. … Continued

Optimize Your Protein Array with Specially Formulated Arraying Buffer

The buffer used for spotting proteins for protein arrays is a critical factor in obtaining reliable and consistent results. Most important impact is on protein aggregation, which can affect spot morphology,  and protein binding to the substrate. Grace Bio-Labs has developed a Protein Arraying Buffer designed to work with our ONCYTE® porous nitrocellulose film slides for optimal … Continued