Tag: Blocking buffer

Optimizing Reverse Phase Protein Array Assays

Components of Reverse-Phase Protein Array (RPPA) methods and their impact on the overall performance of this assay were presented at the 2nd annual RPPA Conference in Edinburgh, Scotland on November 12.  Entire presentation can be viewed here.  View poster here. View here for information regarding our RPPA Assay System Selecting Surfaces, Reagents, and Detection Methods … Continued

Preserving Protein Immuno-Reactivity

Microarrays deposited onto solid supports are robust platforms for performing antibody capture and antigen detection assays.  Among various substrates, thin film porous nitrocellulose cast on glass surfaces has excelled in the field of proteomics since its invention by scientists at Grace Bio-Labs (McGrath, CM, Grudzien, J and A. Levine. Cell Vision 2:499-509, 1995). The irregular 3-dimensional … Continued

From Western Blot to Protein Array with Super G Blocking Buffer

Results obtained from Western blots can be highly variable and dependent not only on the specific antigen/antibody pairs used, but also on other reagents in the protocol such as blocking buffer. Not surprisingly, no one “universal” blocking reagent works well with all proteins. Thus choice of blocking reagent should be assessed for optimal results and having … Continued