Tag: Advancement

Routine Clinical Adoption of Immunoassay Multiplexing, Whether Microarray or Cytometric Bead Array, Is Still Several Years Away Due to Operational Entrenchment

An objective look at immunoassay multiplexing, where it stands today and contrasted to the standard ELISA was authored recently by Dr. Lucy Fairclough et al. The discussion argued the efficacy of multiplexing as well as the pros and cons of present methods available.Commercial availability of multiplex immunoassays for life science discovery research is rapidly expanding … Continued

Targeted Malarial Serological Diagnostics using ONCYTE Porous Nitrocellulose Film Microarray

Targeted diagnostic serological screening using ONCYTE porous nitrocellulose (PNC) microarrays is currently greatly expanding. Interesting research in the Felgner laboratory at UC Irvine has centered on identifying and monitoring P. falciparum antigens involved in malaria infection using subsets of its viral proteome produced via a recombinant expression system and arraying on PNC. Recently, in collaboration … Continued

Optimized Blocking Of Porous Nitrocellulose Films For Sensitive Protein Microarrays

Michael A. Shultz, Aki Ohdera, Jason MacManiman, Charles M. McGrath. Grace Bio-Labs, Inc.   ABSTRACT Super G Blocking Buffer was developed to produce low backgrounds in assays using nitrocellulose film-slides.  Using this blocking reagent in combination with ONCYTE® SuperNOVA Film-slides, we achieved an order of magnitude greater sensitivity in a cytokine microarray assay than with … Continued

Inclusion of Super G in All Steps of Protein Arrays Improves S/N

Blocking reagents are used to reduce non-specific protein binding in immunoassays, significantly improving results in terms of Signal-to-Noise ratio. Typically the blocking reagent is added prior to addition of the primary detection antibody.In this study we asked the question as to whether addition of the blocking reagent to other steps in a protein array assay … Continued

Preserving Protein Immuno-Reactivity

Microarrays deposited onto solid supports are robust platforms for performing antibody capture and antigen detection assays.  Among various substrates, thin film porous nitrocellulose cast on glass surfaces has excelled in the field of proteomics since its invention by scientists at Grace Bio-Labs (McGrath, CM, Grudzien, J and A. Levine. Cell Vision 2:499-509, 1995). The irregular 3-dimensional … Continued

View Your Proteins in 3-D!

Protein microarray technology has progressed significantly and, although new microarray surface chemistries continue to be developed and marketed, porous nitrocellulose has remained the predominant immobilization surface for protein microarray applications due to its many functional advantages.  The 3-dimensional structure of nitrocellulose film slides offers a considerably higher surface area for protein binding compared to conventional … Continued

ProPlate® Chambers Minimize Microarray Variation

Microarray results are susceptible to variation introduced from multiple sources during the course of assay methodologies employed with this technology. A commonly overlooked parameter which may significantly impact results is the choice of chamber/vessel for performing microarray incubations and washes. Chambers which allow for active mixing can significantly improve microarray data. Grace Bio-Labs offers ProPlate® incubation chambers which allow for continuous mixing during microarray assays … Continued

SuperNOVA: Superior Porous Nitrocellulose Surface for Fluorescence Detection

Fluorescence detection is a preferred method for many quantitative protein microarray assays in research and diagnostic applications due to its excellent sensitivity and potential for multiplexing.  Use of multiplexed fluorescence-based assays on porous nitrocellulose though has been hampered by high backgrounds at shorter excitation wavelengths (such as the 532 nm fluorescence channel). We have developed … Continued

Super G: Super Blocking and Signal-to-Noise for ONCYTE Film Slides

Fluorescence detection is a preferred method for many quantitative protein microarray assays in research and diagnostic applications due to its excellent sensitivity and potential for multiplexing. Use of multiplexed fluorescence-based assays on porous nitrocellulose though has been hampered by high backgrounds at shorter excitation wavelengths (such as the 532 nm fluorescence channel).  To maximize the … Continued