Tag: Advancement of proteomics

A Comprehensive Technology Survey of Protein Microarray Platforms, Techniques and Detection Methods

A comprehensive assessment of multiplex immunoassay platforms and detection methods is summarized in an interesting 2011 article by Chandra, et al, in Expert Review Proteomics (1). The article, “Protein Microarrays and Novel Detection Platforms” provides interesting information for several reasons.First, it presents an excellent description of the emerging discovery and clinical need for high throughput … Continued

Preserving Protein Immuno-Reactivity

Microarrays deposited onto solid supports are robust platforms for performing antibody capture and antigen detection assays.  Among various substrates, thin film porous nitrocellulose cast on glass surfaces has excelled in the field of proteomics since its invention by scientists at Grace Bio-Labs (McGrath, CM, Grudzien, J and A. Levine. Cell Vision 2:499-509, 1995). The irregular 3-dimensional … Continued

Protein Microarrays on the Brink?

Microarray technology was first exploited with genomic analysis of DNA sequences.  This technology still forms a robust platform for biomarker discovery and comparative genomics. However, while genomics sheds light on the biological potential, a recent review in Science Signaling  points out that  “protein does the heavy lifting in the cell, and neither DNA nor RNA says much … Continued