APPLICATIONS:
- Microscopy/Imaging
- FRET (Fluorescence Resonance Energy Transfer)
- SPR (Surface Plasmon Resonance)
- Immunohistochemistry
- Protein Crystallization
- Single cell analysis
- FISH
SEALS AND CHAMBERS
Hybridization and Incubation Chambers are designed to confine samples or specimens on glass coverslips, glass slides or other flat surfaces. Multi-chamber/well options allow compartmentalization for simultaneous analysis and processing of several different samples. Numerous conformations for well shape, size and different materials, such as silicone, polycarbonate etc. are available to meet the needs of the different experimental settings and diagnostic requirements.
Our chambers provide devices meeting the compatibility needs for robotic liquid handling for high throughput as well as for smaller scale experiments. Some key features include:
- Bio-compatibility, chemical, and thermal resistance
- Rapid and economical set-up
- Compatible with fluorescent probe.
- Stable, durable seal or removable and reusable chamber options available
- Volume options available for reagent preservation or active mixing
If you do not see the seal or chamber you require below, please contact us for assistance in selecting the correct product for your application or to discuss a custom device
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Hybridization and incubation Seals ad Chambers from Grace Bio-Labs are ideally suited for in situ-hybridization assays. The adhesive seal of SecureSeal™ chambers, for example, bonds to glass and is temperature resistant even after heating. Reactants may be added and removed through seal-able ports. All materials used in SecureSeal™ are compatible with fluorescent, radiometric or enzymatic detection methods.
HybriSlips™ are rigid, light-weight, thin plastic coverslips that minimize friction and facilitate uniform reagent distribution during incubation steps which require small reagent volume. HybriSlips™ are RNase and DNase free, hydrophobic covers that will not trap or bind probes to their surfaces like coverglass. Working surfaces are protected from nuclease contamination by clean liners and are ready to use without pretreatment. HybriSlips™ remain flat and unlike Parafilm, will not curl, even at high temperatures. HybriSlips™ are clear, will not chip or break, and are lighter weight than glass, minimizing friction and facilitating uniform reagent distribution.
HybriWell™ Sealing System bonds securely to a microscope slide surface in seconds to confine small reagent volumes with samples and eliminate evaporation. Each package includes illustrated instructions for use, applicator and 200 adhesive port seals. Any of the designs listed may be easily customized to increase or decrease chamber depth. Hybriwells™ are recommended for most protein and nucleic acid assays. If you are using Cy5 or ® Alexa Fluor 647 direct-labeled DNA probes please refer to our new “fluorescent friendly” chambers. All current designs may be fabricated using our "fluorescent friendly" adhesive material.
SecureSeal™ Hybridization Chambers are thin, silicone-gasketed chambers providing optimal surface-to-volume fluid dynamics for hybridization assays on large or multiple specimens and microarrays on glass or film coated slides. These adhesive-backed chambers are designed for single-use, very-low volume incubations and are ideal for autoradiographic, fluorescent or chemiluminescent end-point applications requiring small, enclosed fluid volumes. SecureSeal™ Adhesive is a double sided tape with a silicone adhesive surface on one side (covered with a clear PET liner) and an acrylic adhesive on the other surface (covered with a paper liner).
