WHOLE PROTEOME PROFILING

 

 

Profiling Pathogen Infection Biomarker Identification Drug Discovery

Antigen microarrays using ONCYTE® Nitrocellulose microarray slides allow comprehensive screening of patient or other blood-derived samples for antibody responses to panels of known or potential antigenic proteins. With the advent of complete genomic sequencing of bacterial and viral agents, it is now possible to express virtually every protein encoded within an organism’s genome, which in turn allows simultaneous screening for antigenic reactivity from samples of known or unknown disposition. ONCYTE® Nitrocellulose microarray slides are the preferred solution to maximize performance in protein microarray assays, due to intrinsic properties not found in other microarray substrates.  This opens the potential to unlock disease pathway mechanisms, reveal details of immune responses and resistance, identify antigens suitable for vaccine development, and to create new types of diagnostic technologies for many infectious conditions currently without effective means of diagnosis.

Advantages of Nitrocellulose Films

ONCYTE® PNC (Porous NitroCellulose) Microarray Slides bind up to 500x more protein in a given spot than planar microarray substrates, resulting in a much higher capacity to capture analyte from a sample solution.  Further, the non-covalent nature of protein binding to ONCYTE Nitrocellulose film slides and the 3-dimensional support matrix results in a protein friendly environment, allowing maintenance of native protein structure and retention of structure dependent binding activity. Nitrocellulose film microarray slides are compatible with existing array printing systems and processes and can be used in multi-chamber isolation systems to perform multiple assays simultaneously for the highest value and throughput processing.