Silicone Wound Splints
Silicone wound splints include suture sites for increased precision in affixing on or within an animal model. These devices enable reproducible and reliable experimental conditions for wound healing. The splints are offered in a variety of shapes and sizes, shipped sterile and ready to use.
We can easily customize shape, size, suture options and depth for your application.
References
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https://gracebio.com/wp-content/plugins/zotpress/
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Choi, Wooil, Dong Jun Park, Robert A. Dorschner, Keita Nakatsutsumi, Michelle Yi, and Brian P. Eliceiri. “CDK1-Loaded Extracellular Vesicles Promote Cell Cycle to Reverse Impaired Wound Healing in Diabetic Obese Mice.” Molecular Therapy 33, no. 3 (March 5, 2025): 1118–33. https://doi.org/10.1016/j.ymthe.2025.01.039.
Zhu, Yi, Ou Mei, Hui Zhang, Wulin You, Jiamin Zhong, Caralyn P. Collins, Guowei Shen, et al. “Establishment and Characterization of a Rat Model of Scalp–Cranial Composite Defect for Multilayered Tissue Engineering.” International Journal of Surgery 110, no. 12 (December 2024): 8233. https://doi.org/10.1097/JS9.0000000000002108.
MAP Schuh, Christina, Fernando Ezquer, Sigde Mamani, Paola R. Campodónico, Constanza Cárcamo, Fabián Martinez-Gómez, Isabel Aburto, Marcelo Ezquer, Bernardo Morales, and Belén Olivares. “A Natural Deep Eutectic Solvent as an Effective Material for Dual Debridement and Antibiofilm Effects in Chronic Wound Treatment.” International Journal of Pharmaceutics 663 (September 30, 2024): 124553. https://doi.org/10.1016/j.ijpharm.2024.124553.
Harrison, Julia M., Edwin K. Leong, Natasha D. Osborne, Jean S. Marshall, and Michael Bezuhly. “AT2R Activation Improves Wound Healing in a Preclinical Mouse Model.” Biomedicines 12, no. 6 (June 2024): 1238. https://doi.org/10.3390/biomedicines12061238.
Safoine, Meryem, Caroline Paquette, Gabrielle-Maude Gingras, and Julie Fradette. “Improving Cutaneous Wound Healing in Diabetic Mice Using Naturally Derived Tissue-Engineered Biological Dressings Produced under Serum-Free Conditions.” Stem Cells International 2024, no. 1 (2024): 3601101. https://doi.org/10.1155/2024/3601101.
Li, Yanqun, Dongyu Liu, Fengming Tan, Wenling Yin, and Zhihuan Li. “Umbilical Cord Derived Mesenchymal Stem Cell-GelMA Microspheres for Accelerated Wound Healing.” Biomedical Materials 18, no. 1 (December 2022): 015019. https://doi.org/10.1088/1748-605X/aca947.
De Pieri, Andrea, Stefanie H. Korntner, Hector Capella-Monsonis, Dimitrios Tsiapalis, Sergei V. Kostjuk, Semyon Churbanov, Peter Timashev, Alexander Gorelov, Yuri Rochev, and Dimitrios I. Zeugolis. “Macromolecular Crowding Transforms Regenerative Medicine by Enabling the Accelerated Development of Functional and Truly Three-Dimensional Cell Assembled Micro Tissues.” Biomaterials 287 (August 1, 2022): 121674. https://doi.org/10.1016/j.biomaterials.2022.121674.
Zbinden, Jacob C., Gabriel J. M. Mirhaidari, Kevin M. Blum, Andrew J. Musgrave, James W. Reinhardt, Christopher K. Breuer, and Jenny C. Barker. “The Lysosomal Trafficking Regulator Is Necessary for Normal Wound Healing.” Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society 30, no. 1 (January 2022): 82–99. https://doi.org/10.1111/wrr.12984.
Verly, Myriam, Emily Mason, Sara Sheikh-Oleslami, Reza Jalili, Breshell Russ, Ruhangiz Taghi Kilani, and Aziz Ghahary. “A Pilot Trial Assessing the Feasibility and Efficacy of a Novel Powder for Rapid Wound Healing.” European Burn Journal 2, no. 4 (December 2021): 238–48. https://doi.org/10.3390/ebj2040018.
Yang, Jee Myung, Junghwa Ryu, Injune Kim, Hak Chang, and Il-Kug Kim. “Dll4 Blockade Promotes Angiogenesis in Nonhealing Wounds of Sox7-Deficient Mice.” Advances in Wound Care 9, no. 11 (November 2020): 591–601. https://doi.org/10.1089/wound.2019.1015.
Nascimento-Filho, Carlos H. V., Ericka J. D. Silveira, Eny M. Goloni-Bertollo, Lelia Batista de Souza, Cristiane H. Squarize, and Rogerio M. Castilho. “Skin Wound Healing Triggers Epigenetic Modifications of Histone H4.” Journal of Translational Medicine 18, no. 1 (March 26, 2020): 138. https://doi.org/10.1186/s12967-020-02303-1.
Kaymakcalan, Omer E., Andrew Abadeer, Josef W. Goldufsky, Uri Galili, Sarah J. Karinja, Xue Dong, Julia L. Jin, Arash Samadi, and Jason A. Spector. “Topical α-Gal Nanoparticles Accelerate Diabetic Wound Healing.” Experimental Dermatology 29, no. 4 (2020): 404–13. https://doi.org/10.1111/exd.14084.
Kellar, Robert S., Robert B. Diller, Aaron J. Tabor, Dominic D. Dominguez, Robert G. Audet, Tatum A. Bardsley, Alyssa J. Talbert, Nathan D. Cruz, Alison L. Ingraldi, and Burt D. Ensley. “Improved Wound Closure Rates and Mechanical Properties Resembling Native Skin in Murine Diabetic Wounds Treated with a Tropoelastin and Collagen Wound Healing Device.” Journal of Diabetes and Clinical Research 2, no. 3 (2020): 86–99. https://doi.org/10.33696/diabetes.1.024.
Lim, Natalie Sheng Jie, Adeline Sham, Stella Min Ling Chee, Casey Chan, and Michael Raghunath. “Combination of Ciclopirox Olamine and Sphingosine-1-Phosphate as Granulation Enhancer in Diabetic Wounds.” Wound Repair and Regeneration 24, no. 5 (2016): 795–809. https://doi.org/10.1111/wrr.12463.
Jin, Enze, Tae-Hee Kim, Seongho Han, and Sung-Whan Kim. “Amniotic Epithelial Cells Promote Wound Healing in Mice through High Epithelialization and Engraftment.” Journal of Tissue Engineering and Regenerative Medicine 10, no. 7 (2016): 613–22. https://doi.org/10.1002/term.2069.
Chereddy, Kiran Kumar, Alessandra Lopes, Salome Koussoroplis, Valéry Payen, Claudia Moia, Huijun Zhu, Pierre Sonveaux, et al. “Combined Effects of PLGA and Vascular Endothelial Growth Factor Promote the Healing of Non-Diabetic and Diabetic Wounds.” Nanomedicine: Nanotechnology, Biology and Medicine 11, no. 8 (November 1, 2015): 1975–84. https://doi.org/10.1016/j.nano.2015.07.006.
Thandavarayan, Rajarajan A., Venkata Naga Srikanth Garikipati, Darukeshwara Joladarashi, Sahana Suresh Babu, Prince Jeyabal, Suresh K. Verma, Alexander R. Mackie, et al. “Sirtuin-6 Deficiency Exacerbates Diabetes-Induced Impairment of Wound Healing.” Experimental Dermatology 24, no. 10 (2015): 773–78. https://doi.org/10.1111/exd.12762.
Park, Shin Ae, Jill Covert, Leandro Teixeira, Monica J Motta, Sara L DeRemer, Nicholas L Abbott, Richard Dubielzig, et al. “Importance of Defining Experimental Conditions in a Mouse Excisional Wound Model.” Wound Repair and Regeneration 23, no. 2 (2015): 251–61. https://doi.org/10.1111/wrr.12272.
Keller, Krista A., Joanne Paul-Murphy, Scott Weber, Philip H. Kass, David Sanchez-Migallon Guzman, Shin Ae Park, Vijay Krishna Raghunathan, Kate A. Gustavsen, and Christopher J. Murphy. “ASSESSMENT OF PLATELET-DERIVED GROWTH FACTOR USING A SPLINTED FULL THICKNESS DERMAL WOUND MODEL IN BEARDED DRAGONS (POGONA VITTICEPS).” Journal of Zoo and Wildlife Medicine 45, no. 4 (December 2014): 866–74. https://doi.org/10.1638/2014-0037.1.
Chereddy, Kiran Kumar, Charles-Henry Her, Michela Comune, Claudia Moia, Alessandra Lopes, Paolo E. Porporato, Julie Vanacker, et al. “PLGA Nanoparticles Loaded with Host Defense Peptide LL37 Promote Wound Healing.” Journal of Controlled Release 194 (November 28, 2014): 138–47. https://doi.org/10.1016/j.jconrel.2014.08.016.
Park, Shin Ae, Vijay Krishna Raghunathan, Nihar M. Shah, Leandro Teixeira, Monica J. Motta, Jill Covert, Richard Dubielzig, et al. “PDGF-BB Does Not Accelerate Healing in Diabetic Mice with Splinted Skin Wounds.” PLOS ONE 9, no. 8 (August 14, 2014): e104447. https://doi.org/10.1371/journal.pone.0104447.
Steinstraesser, Lars, Martin C. Lam, Frank Jacobsen, Paolo E. Porporato, Kiran Kumar Chereddy, Mustafa Becerikli, Ingo Stricker, et al. “Skin Electroporation of a Plasmid Encoding hCAP-18/LL-37 Host Defense Peptide Promotes Wound Healing.” Molecular Therapy 22, no. 4 (April 1, 2014): 734–42. https://doi.org/10.1038/mt.2013.258.
Park, Shin Ae, Leandro B. C. Teixeira, Vijay Krishna Raghunathan, Jill Covert, Richard R. Dubielzig, Roslyn Rivkah Isseroff, Michael Schurr, Nicholas L. Abbott, Jonathan McAnulty, and Christopher J. Murphy. “Full-Thickness Splinted Skin Wound Healing Models in Db/Db and Heterozygous Mice: Implications for Wound Healing Impairment.” Wound Repair and Regeneration 22, no. 3 (2014): 368–80. https://doi.org/10.1111/wrr.12172.
Chereddy, Kiran Kumar, Régis Coco, Patrick B. Memvanga, Bernard Ucakar, Anne des Rieux, Gaëlle Vandermeulen, and Véronique Préat. “Combined Effect of PLGA and Curcumin on Wound Healing Activity.” Journal of Controlled Release 171, no. 2 (October 28, 2013): 208–15. https://doi.org/10.1016/j.jconrel.2013.07.015.
Tanaka, Rica, Max Vaynrub, Haruchika Masuda, Rie Ito, Michiru Kobori, Muneo Miyasaka, Hiroshi Mizuno, Stephen M. Warren, and Takayuki Asahara. “Quality-Control Culture System Restores Diabetic Endothelial Progenitor Cell Vasculogenesis and Accelerates Wound Closure.” Diabetes 62, no. 9 (August 15, 2013): 3207–17. https://doi.org/10.2337/db12-1621.
Wang, Xusheng, Jianfeng Ge, Edward E. Tredget, and Yaojiong Wu. “The Mouse Excisional Wound Splinting Model, Including Applications for Stem Cell Transplantation.” Nature Protocols 8, no. 2 (February 2013): 302–9. https://doi.org/10.1038/nprot.2013.002.
Tan, Nguan S., and Walter Wahli. “Studying Wound Repair in the Mouse.” Current Protocols in Mouse Biology 3, no. 3 (2013): 171–85. https://doi.org/10.1002/9780470942390.mo130135.
Jin, Enze, Jong-Min Kim, and Sung-Whan Kim. “Priming of Mononuclear Cells with a Combination of Growth Factors Enhances Wound Healing via High Angiogenic and Engraftment Capabilities.” Journal of Cellular and Molecular Medicine 17, no. 12 (2013): 1644–51. https://doi.org/10.1111/jcmm.12152.
Chan, Yuk Cheung, Sashwati Roy, Yue Huang, Savita Khanna, and Chandan K. Sen. “The MicroRNA miR-199a-5p Down-Regulation Switches on Wound Angiogenesis by Derepressing the v-Ets Erythroblastosis Virus E26 Oncogene Homolog 1-Matrix Metalloproteinase-1 Pathway *.” Journal of Biological Chemistry 287, no. 49 (November 30, 2012): 41032–43. https://doi.org/10.1074/jbc.M112.413294.
Ganesh, Kasturi, Amitava Das, Ryan Dickerson, Savita Khanna, Narasimham L. Parinandi, Gayle M. Gordillo, Chandan K. Sen, and Sashwati Roy. “Prostaglandin E2 Induces Oncostatin M Expression in Human Chronic Wound Macrophages through Axl Receptor Tyrosine Kinase Pathway.” The Journal of Immunology 189, no. 5 (September 1, 2012): 2563–73. https://doi.org/10.4049/jimmunol.1102762.
Steinstraesser, Lars, Tobias Hirsch, Matthias Schulte, Maximilian Kueckelhaus, Frank Jacobsen, Evgenija A. Mersch, Ingo Stricker, et al. “Innate Defense Regulator Peptide 1018 in Wound Healing and Wound Infection.” PLOS ONE 7, no. 8 (August 6, 2012): e39373. https://doi.org/10.1371/journal.pone.0039373.
Kim, Sung-Whan, Hong-Zhe Zhang, Longzhe Guo, Jong-Min Kim, and Moo Hyun Kim. “Amniotic Mesenchymal Stem Cells Enhance Wound Healing in Diabetic NOD/SCID Mice through High Angiogenic and Engraftment Capabilities.” PLOS ONE 7, no. 7 (July 17, 2012): e41105. https://doi.org/10.1371/journal.pone.0041105.
Shin, Laura, and Daniel A. Peterson. “Impaired Therapeutic Capacity of Autologous Stem Cells in a Model of Type 2 Diabetes.” Stem Cells Translational Medicine 1, no. 2 (February 1, 2012): 125–35. https://doi.org/10.5966/sctm.2012-0031.
Perez-Aso, Miguel, Luis Chiriboga, and Bruce N. Cronstein. “Pharmacological Blockade of Adenosine A2A Receptors Diminishes Scarring.” The FASEB Journal 26, no. 10 (2012): 4254–63. https://doi.org/10.1096/fj.12-209627.
Greives, Matthew R., Fares Samra, Savvas C. Pavlides, Keith M. Blechman, Sara-Megumi Naylor, Christopher D. Woodrell, Caprice Cadacio, et al. “Exogenous Calreticulin Improves Diabetic Wound Healing.” Wound Repair and Regeneration 20, no. 5 (2012): 715–30. https://doi.org/10.1111/j.1524-475X.2012.00822.x.
Wong, Victor W., Michael Sorkin, Jason P. Glotzbach, Michael T. Longaker, and Geoffrey C. Gurtner. “Surgical Approaches to Create Murine Models of Human Wound Healing.” Edited by Andrea Vecchione. BioMed Research International 2011, no. 1 (2011): 969618. https://doi.org/10.1155/2011/969618.
Tian, Haibin, Yan Lu, Shraddha P. Shah, and Song Hong. “Novel 14S,21-Dihydroxy-Docosahexaenoic Acid Rescues Wound Healing and Associated Angiogenesis Impaired by Acute Ethanol Intoxication/Exposure.” Journal of Cellular Biochemistry 111, no. 2 (2010): 266–73. https://doi.org/10.1002/jcb.22709.
Nguyen, Phuong D., John Paul Tutela, Vishal D. Thanik, Denis Knobel, Robert J. Allen Jr., Christopher C. Chang, Jamie P. Levine, Stephen M. Warren, and Pierre B. Saadeh. “Improved Diabetic Wound Healing through Topical Silencing of P53 Is Associated with Augmented Vasculogenic Mediators.” Wound Repair and Regeneration 18, no. 6 (2010): 553–59. https://doi.org/10.1111/j.1524-475X.2010.00638.x.
Thangarajah, Hariharan, Dachun Yao, Edward I. Chang, Yubin Shi, Leila Jazayeri, Ivan N. Vial, Robert D. Galiano, et al. “The Molecular Basis for Impaired Hypoxia-Induced VEGF Expression in Diabetic Tissues.” Proceedings of the National Academy of Sciences 106, no. 32 (August 11, 2009): 13505–10. https://doi.org/10.1073/pnas.0906670106.
Lin, Clarence D., Alexander C. Allori, Jared E. Macklin, Alexander M. Sailon, Rica Tanaka, Jamie P. Levine, Pierre B. Saadeh, and Stephen M. Warren. “Topical Lineage-Negative Progenitor-Cell Therapy for Diabetic Wounds.” Plastic and Reconstructive Surgery 122, no. 5 (November 2008): 1341. https://doi.org/10.1097/PRS.0b013e318188217b.
Le, Huong, Rebecca Kleinerman, Oren Z. Lerman, Daniel Brown, Robert Galiano, Geoffrey C. Gurtner, Stephen M. Warren, Jamie P. Levine, and Pierre B. Saadeh. “Hedgehog Signaling Is Essential for Normal Wound Healing.” Wound Repair and Regeneration 16, no. 6 (2008): 768–73. https://doi.org/10.1111/j.1524-475X.2008.00430.x.
Michaels, Joseph, Samara S. Churgin, Keith M. Blechman, Matthew R. Greives, Shahram Aarabi, Robert D. Galiano, and Geoffrey C. Gurtner. “Db/Db Mice Exhibit Severe Wound-Healing Impairments Compared with Other Murine Diabetic Strains in a Silicone-Splinted Excisional Wound Model.” Wound Repair and Regeneration 15, no. 5 (2007): 665–70. https://doi.org/10.1111/j.1524-475X.2007.00273.x.
Galiano, Robert D., Oren M. Tepper, Catherine R. Pelo, Kirit A. Bhatt, Matthew Callaghan, Nicholas Bastidas, Stuart Bunting, Hope G. Steinmetz, and Geoffrey C. Gurtner. “Topical Vascular Endothelial Growth Factor Accelerates Diabetic Wound Healing through Increased Angiogenesis and by Mobilizing and Recruiting Bone Marrow-Derived Cells.” The American Journal of Pathology 164, no. 6 (June 1, 2004): 1935–47. https://doi.org/10.1016/S0002-9440(10)63754-6.
Galiano, Robert D., Joseph Michaels V, Michael Dobryansky, Jamie P. Levine, and Geoffrey C. Gurtner. “Quantitative and Reproducible Murine Model of Excisional Wound Healing.” Wound Repair and Regeneration 12, no. 4 (2004): 485–92. https://doi.org/10.1111/j.1067-1927.2004.12404.x.
Kaneko, Yuka. “Topical Application of Nano-Chitin Accelerates Wound Healing in Diabetic Mice,” n.d.
“Nephrin Expression in Human Epidermal Keratinocytes and Its Implication in Poor Wound Closure.” Accessed March 31, 2025. https://doi.org/10.1096/fj.202100455RR.
Guan, Ya, Min Zhang, Jiyeon Song, Marcos Negrete, Tyler Adcock, Reeva Kandel, Luigi Racioppi, and Sharon Gerecht. “CaMKK2 Regulates Macrophage Polarization Induced by Matrix Stiffness: Implications for Shaping the Immune Response in Stiffened Tissues.” Advanced Science n/a, no. n/a: 2417778. Accessed March 17, 2025. https://doi.org/10.1002/advs.202417778.
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