Protein Crystallization
PROCRYSTAL™ PROTEIN CRYSTALLIZATION COVERS
ProCrystal™ protein crystallization covers are designed for high throughput protein x-ray crystallography in 96-well plates. The ProCrystal ™ material maintains drop footprint and segregation, even with protocols using MPD, glycerol or detergents. Grace Bio-Labs has collaborated with leading crystallographers to develop our ProCrystal™ covers using materials with the highest optical quality and stringent manufacturing processes to ensure clean, particle-free covers.
Features Include:
- Ready-to-use, dust-free
- UV compatible
- No noticeable x-ray diffraction
- Accommodates up to 3 protein drops per well
- Two formats: pre-cut or uncut
ProCrystal™ protein crystallization covers accommodate up to 3 protein drops per well, and are available as frame or frameless, with either paper or plastic liners, and in two cut formats: pre-cut or uncut (see Specifications tab). The pre-cut format allows removal of covers from individual wells at different times. Our protein crystallization covers are compatible with SBS-compliant microtiter plates and TTP Mosquito(r) Instruments.
Applications
Optimal for high throughput protein x-ray crystallography in 96-well plates.
Protein crystallization is a key assay for structural studies of proteins. The protocols for crystallization of protein are challenging due to the stringent requirement for pure samples and control of environmental conditions during the crystallization process. Vapor diffusion using hanging drop is a preferred method for obtaining quality crystals with high throughput. This protocol requires a coverslip (or protein crystallization cover) on which a drop of protein solution is placed on top of a reagent reservoir. The progression of crystal formation is observed through the cover, and then the cover is removed to capture the crystals. This process is often conducted in multiwell (96-well) plate format for high throughput screening.
