Silicone Isolators allow researchers to isolate specimens using removable hydrophobic barriers. They may be used to isolate cells grown in culture dishes or separate multiple specimens affixed to microscope slides. Isolators are available in red silicone thickness 0.5 to 2.5mm pre-cut or as sheet material with or without secure seal clean adhesive on one or both surfaces. Nonadhesive isolators are autoclavable and reusable.
Silicone Isolators™ remain sealed to smooth surfaces during washing steps. Where additional sealing is required, SecureSeal™ adhesive on one or both surfaces is recommended. Each seal design is also available in CultureWell™ “water-clear” silicone, which is highly recommended for sensitive cell culture applications. Closed chambers may be formed using flexible RNase free, HybriSlip ™ covers. Recommended use temperature is -62°C to 218°C.
Protein and DNA arrays
Fluorescence In situ Hybridization (FISH)
Biopolymers and hydrogel formulation
Cryogenic-transmission electron microscopy (Cryo-TEM)
Ultra-small-angle X-ray scattering (USAXS)
Live cell lithography” (LCL)
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Li, M. W., Biggers, J. D., Elmoazzen, H. Y., Toner, M., McGinnis, L., & Lloyd, K. C. K. (2007). Long-term storage of mouse spermatozoa after evaporative drying. Reproduction (Cambridge, England), 133(5), 919–929.
Ho, D., Falter, K., Severin, P., & Gaub, H. E. (2009). DNA as a Force Sensor in an Aptamer-Based Biochip for Adenosine. Analytical Chemistry, 81(8), 3159–3164.
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Silicone Isolator Application
Q: Do the non-adhesive reusable isolators adhere to the glass slide and allow a small volume of liquid to be contained? Are the isolators with adhesive more effective?
A: Typically for most applications, the silicone isolator (without PSA) adheres very well and can be removed easily.
FastWells™ Reagent BarriersSEARCH
FastWells™ are sticky, flexible silicone gaskets that form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Gaskets may be stacked to increase depth and volume. FastWell™ Reagent Barriers provide rapid isolation of cells and tissues on slides or coverslips for antibody incubations. The barriers remain sealed during agitated washing steps, preventing reagents from spilling when shaken or rocked. FastWells™ peel off quickly and cleanly, leaving no residue to interfere with specimen cover-slipping. They can be washed, autoclaved and reused. The barriers can also be sealed to form incubation chambers using flexible HybriSlip™ covers.