Protein Crystallization

Protein crystallization is a key assay for structural studies of proteins. The protocols for crystallization of protein are challenging due to the stringent requirement for pure samples and control of environmental conditions during the crystallization process. Vapor diffusion using hanging drop is a preferred method for obtaining quality crystals with…

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Microarray Substrates

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Grace Bio-Labs microarray surface chemistry is based on the well-known protein-binding properties of nitrocellulose. A range of different formulations have…

APPLICATIONS:

Antigen-Capture AssayAntibody Capture AssayRPPA- Reverse Phase Protein MicroarrayLaser micro-dissection RRPAEpitope-mappingBiomarker Discovery and ValidationImmunogen Discovery

Microarray Reagents

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Grace Bio-Labs microarray regents have been specifically formulated to achieve the full potential of porous nitrocellulose, accelerating experimental design and…

APPLICATIONS:

Antigen-Capture Assay Antibody Capture Assay RPPA- Reverse Phase Protein Microarray Laser micro-dissection RRPA Epitope-mapping Biomarker Discovery and Validation Immunogen Discovery

ProPlate®

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ProPlates® were specifically designed to enable automated robotic liquid handling. Two main configurations are available: The ProPlate® Microtiter Plate is comprised…

APPLICATIONS:

ProteomicsProtein MicroarraysProtein expression analysisAntibody profiling cDNA and oligonucleotide arrays

FastWells™ Reagent Barriers

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FastWells™ are sticky, flexible silicone gaskets that form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Gaskets may…

APPLICATIONS:

MicroscopyImagingFluorescence In situ Hybridization (FISH)Single-molecule fluorescence analysisImmunohistochemistry

CoverWell™ Incubation Chambers

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CoverWell™ incubation chambers are reusable, easy to apply chambers that attach without the use of adhesive.  CoverWells™ enclose a large…

APPLICATIONS:

In-situ hybridizationImmunohistochemistryDNA MicroarrayReverse Transfection Microarray

CoverWell Perfusion Chambers

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CoverWell ™ perfusion press-to-seal covers form water-tight, multiwell cell incubation or cytochemistry chambers when pressed to coverslips or microscope slides.…

APPLICATIONS:

Live-cell imagingMicroscopyImagingSingle molecule spectroscopy

HybriSlip™ Hybridization Covers

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HybriSlips™ are rigid, light-weight, thin plastic coverslips that minimize friction and facilitate uniform reagent distribution during incubation steps which require…

APPLICATIONS:

In-situ HybridizationLorem ipsum dolorIn-situ PCRImmunohistochemistryProtein MicroarrayDNA Microarray

Imaging Spacers

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Imaging spacers are ultra-thin adhesive spacers which peel-and-stick to coverglass or microscope slides to confine specimens without compression. Layer multiple…

APPLICATIONS:

ImagingMicroscopyHigh-temperature single-molecule kinetic analysisanti‐Stokes Raman scattering microscopy

SecureSeal™ Hybridization Chambers

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SecureSeal™ Hybridization Chambers are thin, silicone-gasketed chambers providing optimal surface-to-volume fluid dynamics for hybridization assays on large or multiple specimens…

APPLICATIONS:

In situ hybridizationProtein and DNA MicroarraysImmunocytochemistryRapid microfluidic prototypingFluorescence Resonance Energy Transfer (FRET)

CoverWell™ Incubation Chambers

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CoverWell™ incubation chambers are reusable, easy to apply chambers that attach without the use of adhesive.  CoverWells™ enclose a large…

APPLICATIONS:

Reverse Transfection Microarray;  DNA Microarray;  In-situ hybridization;  Immunohistochemistry; 

Silicone Isolators

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Silicone Isolators allow researchers to isolate specimens using removable hydrophobic barriers. They may be used to isolate cells grown in…

SecureSeal™ Hybridization Chambers

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SecureSeal™ Hybridization Chambers are thin, silicone-gasketed chambers providing optimal surface-to-volume fluid dynamics for hybridization assays on large or multiple specimens…

HybriWell™ Sealing System

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HybriWell™ Sealing System bonds securely to a microscope slide surface in seconds to confine small reagent volumes with samples and…

Hybridization and Incubation

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Hybridization and incubation Seals ad Chambers from Grace Bio-Labs are ideally suited for in situ-hybridization assays. The adhesive seal of…

APPLICATIONS:

In-situ hybridization MicroarraysFluorescence In situ Hybridization (FISH)FRET (Fluorescence Resonance Energy Transfer)

HybriWell™ Sealing System 8 Pack

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Chamber 8-Pack Assortment – Includes Port Seals and Applicator

Adhesive Seal Tabs

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Adhesive seal tabs complement SecureSeal™ Hybridization Chambers, HybriWell™ Sealing System and CoverWell™ Perfusion Chambers. Once the paper liner is removed,…

APPLICATIONS:

In situ hybridization ;  Protein and DNA Microarrays ;  Immunocytochemistry ;  DNA Amplification ;  Neurophysiology ;  Reaction kinetics ; 

FastWells™ Reagent Barriers

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FastWells™ are sticky, flexible silicone gaskets that form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Gaskets may…

FlexWell™ 4 Slide Tray

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Multiple slides and FlexWells™ may be inserted into a reusable FlexWell™ tray, organizing wells into the footprint of a SBS…

APPLICATIONS:

Protein MicroarrayHybridizationIncubation

FlexWell™ Incubation Chambers

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FlexWell™ incubation chamber silicone gaskets form wells on slides using clean release adhesive to isolate up to 16 specimens per…

APPLICATIONS:

Protein MicroarrayHybridizationIncubation

FlexWell™ Seal Strips

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To prevent evaporation apply seal strips using the plastic applicator to the top surface of the chamber. Seal strips remove cleanly…

APPLICATIONS:

Microarrays using nitrocellulose coatedHybridizationIncubation

HybriSlip™ Hybridization Covers

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HybriSlips™ are rigid, light-weight, thin plastic coverslips that minimize friction and facilitate uniform reagent distribution during incubation steps which require…

ProPlates®

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ProPlates® were specifically designed to enable automated robotic liquid handling. Two main configurations are available: The ProPlate® Microtiter Plate is comprised…

APPLICATIONS:

ProteomicsProtein MicroarraysProtein expression analysis;  Antibody profiling ;  cDNA and oligonucleotide arrays; 

Silicone Isolators™ Sheet Material

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Silicone isolator™ sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. Where additional sealing is…

APPLICATIONS:

Protein and DNA arrays ;  Immunohistochemistry;  Fluorescence In situ Hybridization (FISH) ;  Biopolymers and hydrogel formulation ;  Cryogenic-transmission electron microscopy (Cryo-TEM) ;  Microwave crystallization ;  Ultra-small-angle X-ray scattering (USAXS) ;  Tissue ingeneering;  Live cell lithography” (LCL); 

Imaging Spacers

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Imaging spacers are ultra-thin adhesive spacers which peel-and-stick to coverglass or microscope slides to confine specimens without compression. Layer multiple…

APPLICATIONS:

Imaging;  Microscopy;  High-temperature single-molecule kinetic analysis;  Anti‐Stokes Raman scattering microscopy; 

CoverWell™ Imaging Chambers

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CoverWell ™ imaging chambers are designed to stabilize and support thick and free-floating specimens for confocal microscopy and imaging applications.…

APPLICATIONS:

Confocal microscopy Imaging Tissue and Cell staining ;  High Resolution Microscopy ;  Live-cell imaging ; 

CoverWell™ Perfusion Chambers

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CoverWell ™ perfusion press-to-seal covers form water-tight, multiwell cell incubation or cytochemistry chambers when pressed to coverslips or microscope slides.…

APPLICATIONS:

Single molecule spectroscopy Live-cell imaging Microscoscopy

FastWells™ Reagent Barriers

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FastWells™ are sticky, flexible silicone gaskets that form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Gaskets may…

APPLICATIONS:

Microscopy Fluorescence In situ Hybridization (FISH) Single-molecule fluorescence analysis ;  Immunohistochemistry ; 

MultiSlip™ Coverglass Inserts

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MutliSlip™ inserts with 8 (18mm x 18mm) or 15 (12mm x 12mm) No. 1.5 German glass coverglass per insert are…

APPLICATIONS:

High resolution microscopy Fluorescent imaging Immunohistochemistry ;  Cell Culture; 

SecureSeal™ Adhesive Sheets

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These adhesive sheets are made using the same SecureSeal™ adhesive as is used to make HybriWell™ and SecureSeal™ Incubation Chambers.  Thin,…

APPLICATIONS:

Imaging ;  Tissue and Cell staining ;  High Resolution Microscopy; 

SecureSlip™ Silicone Supported Coverglass

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SecureSlip™ Silicone Supported Coverglass is affixed to a thin microscopically transparent silicone base which secures it to culture vessels by…

Imaging

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Imaging seals and chambers from Grace Bio-Labs offer a selection of tools for cell/tissue staining for high quality results in…

APPLICATIONS:

Tissue and Cell stainingHigh Resolution MicroscopyLive-cell imaging

Adhesive Seal Tabs

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Adhesive seal tabs complement SecureSeal™ Hybridization Chambers, HybriWell™ Sealing System and CoverWell™ Perfusion Chambers. Once the paper liner is removed,…

APPLICATIONS:

In situ hybridization ;  Protein and DNA Microarrays;  Immunocytochemistry ;  DNA Amplification ;  Neurophysiology ;  Reaction kinetics; 

CultureWell™ Coverglass Removal Tool

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CS16 CultureWell™ removable chambered coverglass is a 16-well chambered coverglass cell culture vessel, with 2 x 8 format with standard…

APPLICATIONS:

Cell culture Fluorescence imaging

CultureWell™ Microplate Adapter Tray

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CultureWell™ Microplate Adapter Tray holds up to 4 CultureWell™ 16 Chambers for higher throughput

CultureWell™ MultiWell Chambered Coverslips

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CultureWell™ chambered coverglass products consist of removable and reusable, non-cytotoxic silicone gaskets secured to number 1.5 German coverglass. Chambered coverglass…

APPLICATIONS:

Cell Culture Fluorescence applications In-situ hybridization Immunostaining

CS16-CultureWell™ Removable Chambered Coverglass

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CS16 CultureWell™ removable chambered coverglass is a 16-well chambered coverglass cell culture vessel, with 2 x 8 format with standard…

APPLICATIONS:

Cell CultureFluorescence applicationsIn-situ hybridizationImmunostaining

CultureWell™ Coverglass Inserts

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Each CultureWell™ coverglass insert is comprised of four chambered coverglass, assembled in a disposable frame placed in a standard 86mm…

APPLICATIONS:

High resolution microscopy Fluorescent imaging Immunohistochemistry

CultureWell™ Reusable Gaskets

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Gaskets are ideal for forming wells on glass microscope slides or in polystyrene dishes. Gaskets are non-sterile and may be…

APPLICATIONS:

Cell CultureHigh resolution microscopyFluorescent imaging Immunohistochemistry

CultureWell™ Silicone Sheet Material

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CultureWell™ clear silicone sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. They may be…

APPLICATIONS:

Cell CultureHigh resolution microscopy Fluorescent imagingImmunohistochemistry

MultiSlip™ Coverglass Inserts

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MutliSlip™ inserts with 8 (18mm x 18mm) or 15 (12mm x 12mm) No. 1.5 German glass coverglass per insert are…

APPLICATIONS:

Cell CultureFluorescent imaging Immunohistochemistry

SecureSlip™ Silicone Supported Coverglass

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SecureSlip™ Silicone Supported Coverglass is affixed to a thin microscopically transparent silicone base which secures it to culture vessels by…

APPLICATIONS:

Cell CultureImmunofluorescence assayMicroscopy

CultureWell™ ChamberSLIP 16, Non-Removable Chambered Coverglass

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CultureWell™ NON Removable Chambered Coverglass, 16 Well, No. 1.5 German borosilicate Coverglass. Product consists of cell culture vessels, with a…

APPLICATIONS:

Cell Culture Fluorescence applicationsSmall volume incubation Immunostaining

Silicone Wound Splints

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Wound splints are constructed of silicone and include suture sites for increased precision in affixing on or within an animal…

Silicone Isolator Sheet Material

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Silicone isolator™ sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. Where additional sealing is…

APPLICATIONS:

Protein and DNA arrays ;  Immunohistochemistry ;  Fluorescence In-situ Hybridization (FISH) ;  Biopolymers and hydrogel formulation;  Cryogenic-transmission electron microscopy (Cryo-TEM) ;  X-ray scattering ;  Microwave crystallization ;  Ultra-small-angle X-ray scattering (USAXS) ;  Tissue engineering Live cell lithography (LCL); 

CultureWell Silicone Sheet Material

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CultureWell™ clear silicone sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. They may be…

APPLICATIONS:

Lorem Ipsum ;  Lorem Ipsum;  Lorem Ipsum; 

ProPlate® Multi-Array Slide System

ProPlate® multi-wells integrate microscope slide-based array technology with automated microtiter plate processing. These products allow users to isolate reagents on virtually any microscope slide surface, quickly and easily, forming up to 64 leak-proof, wells per slide. All ProPlate slide chambers feature 7.5mm depth to provide a generous surface to volume ratio to facilitate mixing and washing steps. Measured drawings illustrate the variety of well sizes and spacing available to fit standard 1 x 25 x 75mm microscope slides. Designs feature center to center spacing and ANSI-SLAS compliant well spacing for use with multichannel pipetting.

Slides may be processed individually or multiple slides may be quickly inserted into a frame having the standard footprint of a microtiter plate for use with liquid handling robotics or automated plate washers. To prevent evaporation during incubation steps, wells may be sealed by means of flexible seals strips or lidded frames. After processing, slides may be quickly disassembled from the ProPlate and read using conventional chip readers. ProPlate materials may be washed and re-used multiple times. ProPlates are compatible with Grace’s ONCYTE ® Film-slide products for protein microarrays and most of the reagents common to use with microarray assays.

ProPlate® multi-wells are comprised of a rigid polycarbonate upper-structure and a compressible silicone gasket. A thin intervening layer of double-sided adhesive between the upper-structure and gasket serve to hold the gasket in position with respect to the alignment of the wells in both components. The adhesive layer does not come into contact with the reagents or sample substrate. Forming a seal between the silicone gasket and the microscope slide is achieved by applying either plastic snap-clips molded from delrin or stainless steel spring clips to the sandwiched assembly as shown in the illustrated instructions. With the exception of the snap-clips, all of the components of the system may be autoclaved and re-used multiple times. Standard chemical reference data for silicone and polycarbonate may be used to identify most chemical compatibilities with respect to reagents.

 

Applications

  • Protein and peptides microarrays
  • cDNA and oligonucleotides microarrays
  • Proteomics
  • Protein expression analysis
  • Antibody profiling

References

Luckert, K., Gujral, T. S., Chan, M., Joos, T. O., Sorger, P. K., MacBeath, G., & Pötz, O. (2012). A Dual Array-Based Approach to Assess the Abundance and Posttranslational Modification State of Signaling Proteins. Science Signaling, 5(206), pl1. 

Nielsen, U. B., Cardone, M. H., Sinskey, A. J., MacBeath, G., & Sorger, P. K. (2003). Profiling receptor tyrosine kinase activation by using Ab microarrays. Proceedings of the National Academy of Sciences, 100(16), 9330–9335.

Guo, S.-L., Chen, P.-C., Chen, M.-S., Cheng, Y.-C., Lin, J.-M., Lee, H.-C., & Chen, C.-S. (2012). A Fast Universal Immobilization of Immunoglobulin G at 4°C for the Development of Array-based Immunoassays. PLoS ONE, 7(12). 

To see more references related to this product click here.

Videos

Microarray Hybridization with ProPlate

 

ProPlate Assembly & Disassembly Stainless Steel Spring Clips

ProPlate Assembly & Disassemly Delrin Snap Clips

FAQ

Q:   We would like to use the ProPlate (16 well module) system for culturing cells. Can we use ProPlate for this application, and how are they best sterilized?

A: You can autoclave the ProPlate materials, after first removing the thin adhesive layer between the silicone and the polycarbonate superstructure. Do not autoclave the delrin snap clips. Stainless steel spring clips may be autoclaved. After careful assembly in the laminar flow hood, ProPlate modules are inserted into culture dishes, lidded and placed into a CO2 incubator. I have done this many times, my media usually contains Pen/Strep.

ProPlate® Delrin Snap Clips, Non-Numbered

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ProPlate Delrin Snap Clips are easily applied and removed, allowing up to 4 chambered slides to be inserted into a tray.

ProPlate® Microtiter Plates

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Patented ProPlate® MTP technology for high throughput robotic processing of microarrays allows you to combine your content arrayed substrate to a disposable ANSI-SLAS -compliant microtiter plate.

ProPlate® Multi-Array Slide System

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ProPlate® re-usable, multi-well chambers form removable, leak-proof wells on virtually any slide surface without the use of adhesive. Designs feature ANSI-SLAS compliant well spacing to facilitate multichannel pipetting. Multiple slides insert easily into frames having a standard microtiter plate footprint for automated processing.

ProPlate® Multi-Well Chambers

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ProPlate® re-usable, multi-well chambers are available in a wide variety of formats to fit standard 1 x 25 x 75 mm glass microscope slide substrates. Chambers having a depth of approximately 7.5 mm provide a generous surface to volume ratio to facilitate mixing and washing steps.

ProPlate® Slide Module Seal Strip Applicator

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The plastic applicator helps to apply seal strips to the top surface of the chambers. Seal strips are ideal to prevent evaporation, they remove cleanly and easily without leaving a residue.

ProPlate® Slide Module Seal Strips

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To prevent evaporation apply seal strips using the plastic applicator to the top surface of the chamber. Seal strips remove cleanly and easily without leaving a residue.

These adhesive strips are manufactured from materials used to seal PCR plates and compatible with hybridization reagents without contamination. Includes 50 Seal Strips and 1 Applicator

ProPlate® Stainless Steel Spring Clips, Numbered

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ProPlate® stainless steel Spring Clips are easily applied and removed and are preferred by customers using glass slides fabricated by means of a laser and which may have small micro-fractures from processing or chipped edges.

ProPlate® Stainless Steel, Spring Clips, Non-Numbered

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ProPlate® stainless steel Spring Clips are easily applied and removed and are preferred by customers using glass slides fabricated by means of a laser and which may have small micro-fractures from processing or chipped edges.

ProPlate® Tray and Cover for Delrin Snap Clip Modules

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Organize up to 4 chambered slides into the footprint of a microtiter plate for use with automated liquid handling robots or plate washers.

ProPlate® Tray and Cover for Spring Clip Modules

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Organize up to 3 chambered slides into the footprint of a microtiter plate for use with automated liquid handling robots or plate washers.