ProPlate® Multi-Array Slide System
ProPlate® Multi-Array Slide System integrates microscope slide-based array technology with automated microtiter plate processing. These products comprise a frame holding 3 or 4 ProPlate® multi-wells chambers. These chambers allow users to isolate reagents on virtually any microscope slide surface, quickly and easily, forming up to 64 leak-proof, wells per slide.
All ProPlate ProPlate® multi-wells chambers feature:
- a generous surface to volume ratio for optimal mixing capacity
- ANSI-SLAS compliant well spacing for multichannel pipetting and robotic liquid handling
- seals strips or lidded frames prevent evaporation and cross-contamination
- compatible with ONCYTE® and Protein Microarray Slides and Grace Bio-Labs’ Microarray Reagents
- available with delrin snap-clips or stainless steel spring clips
- several configurations
- removable, autoclavable∗ and re-usable
∗ ProPlate trays and delrin snap-clips are not autoclavable.
Technical Resources
Instructions
Instructions for Use - Assembling ProPlate Slide Modules with Delrin clips
Instructions for Use - Assembling ProPlate Slide Modules with Stainless Steel Spring Clips
Applications
- Protein and peptides microarrays
- cDNA and oligonucleotides microarrays
- Proteomics
- Protein expression analysis
- Antibody profiling
References
Luckert, K., Gujral, T. S., Chan, M., Joos, T. O., Sorger, P. K., MacBeath, G., & Pötz, O. (2012). A Dual Array-Based Approach to Assess the Abundance and Posttranslational Modification State of Signaling Proteins. Science Signaling, 5(206), pl1.
Nielsen, U. B., Cardone, M. H., Sinskey, A. J., MacBeath, G., & Sorger, P. K. (2003). Profiling receptor tyrosine kinase activation by using Ab microarrays. Proceedings of the National Academy of Sciences, 100(16), 9330–9335.
Guo, S.-L., Chen, P.-C., Chen, M.-S., Cheng, Y.-C., Lin, J.-M., Lee, H.-C., & Chen, C.-S. (2012). A Fast Universal Immobilization of Immunoglobulin G at 4°C for the Development of Array-based Immunoassays. PLoS ONE, 7(12).
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Videos
Microarray Hybridization with ProPlate
ProPlate Assembly & Disassembly Stainless Steel Spring Clips
ProPlate Assembly & Disassemly Delrin Snap Clips
FAQ
Q: We would like to use the ProPlate (16 well module) system for culturing cells. Can we use ProPlate for this application, and how are they best sterilized?
A: You can autoclave the ProPlate materials, after first removing the thin adhesive layer between the silicone and the polycarbonate superstructure. Do not autoclave the delrin snap clips. Stainless steel spring clips may be autoclaved. After careful assembly in the laminar flow hood, ProPlate modules are inserted into culture dishes, lidded and placed into a CO2 incubator. I have done this many times, my media usually contains Pen/Strep.