ProPlate® Multi-Array Slide System
ProPlate® Multi-Array Slide System integrates microscope slide-based array technology with automated microtiter plate processing. These products comprise a frame holding 3 or 4 ProPlate® multi-wells chambers. These chambers allow users to isolate reagents on virtually any microscope slide surface, quickly and easily, forming up to 64 leak-proof, wells per slide.
All ProPlate ProPlate® multi-wells chambers feature:
- a generous surface to volume ratio for optimal mixing capacity
- ANSI-SLAS compliant well spacing for multichannel pipetting and robotic liquid handling
- seals strips or lidded frames prevent evaporation and cross-contamination
- compatible with ONCYTE® and Protein Microarray Slides and Grace Bio-Labs’ Microarray Reagents
- available with delrin snap-clips or stainless steel spring clips
- several configurations
- removable, autoclavable∗ and re-usable
∗ ProPlate trays and delrin snap-clips are not autoclavable.
Technical Resources
Instructions
Instructions for Use - Assembling ProPlate Slide Modules with Delrin clips
Instructions for Use - Assembling ProPlate Slide Modules with Stainless Steel Spring Clips
Applications
- Protein and peptides microarrays
- cDNA and oligonucleotides microarrays
- Proteomics
- Protein expression analysis
- Antibody profiling
References
Luckert, K., Gujral, T. S., Chan, M., Joos, T. O., Sorger, P. K., MacBeath, G., & Pötz, O. (2012). A Dual Array-Based Approach to Assess the Abundance and Posttranslational Modification State of Signaling Proteins. Science Signaling, 5(206), pl1.
Nielsen, U. B., Cardone, M. H., Sinskey, A. J., MacBeath, G., & Sorger, P. K. (2003). Profiling receptor tyrosine kinase activation by using Ab microarrays. Proceedings of the National Academy of Sciences, 100(16), 9330–9335.
Guo, S.-L., Chen, P.-C., Chen, M.-S., Cheng, Y.-C., Lin, J.-M., Lee, H.-C., & Chen, C.-S. (2012). A Fast Universal Immobilization of Immunoglobulin G at 4°C for the Development of Array-based Immunoassays. PLoS ONE, 7(12).
To see more references related to this product click here.
Videos
Microarray Hybridization with ProPlate
ProPlate Assembly & Disassembly Stainless Steel Spring Clips
ProPlate Assembly & Disassemly Delrin Snap Clips
FAQ
Q: We would like to use the ProPlate (16 well module) system for culturing cells. Can we use ProPlate for this application, and how are they best sterilized?
A: You can autoclave the ProPlate materials, after first removing the thin adhesive layer between the silicone and the polycarbonate superstructure. Do not autoclave the delrin snap clips. Stainless steel spring clips may be autoclaved. After careful assembly in the laminar flow hood, ProPlate modules are inserted into culture dishes, lidded and placed into a CO2 incubator. I have done this many times, my media usually contains Pen/Strep.
ProPlate® re-usable, multi-well chambers form removable, leak-proof wells on virtually any slide surface without the use of adhesive. Designs feature ANSI-SLAS compliant well spacing to facilitate multichannel pipetting. Multiple slides insert easily into frames having a standard microtiter plate footprint for automated processing.
ProPlate® re-usable, multi-well chambers are available in a wide variety of formats to fit standard 1 x 25 x 75 mm glass microscope slide substrates. Chambers having a depth of approximately 7.5 mm provide a generous surface to volume ratio to facilitate mixing and washing steps.
Patented ProPlate® MTP technology for high throughput robotic processing of microarrays allows you to combine your content arrayed substrate to a disposable ANSI-SLAS -compliant microtiter plate.
ProPlate Delrin Snap Clips are easily applied and removed, allowing up to 4 chambered slides to be inserted into a tray.
The plastic applicator helps to apply seal strips to the top surface of the chambers. Seal strips are ideal to prevent evaporation, they remove cleanly and easily without leaving a residue.
To prevent evaporation apply seal strips using the plastic applicator to the top surface of the chamber. Seal strips remove cleanly and easily without leaving a residue.
These adhesive strips are manufactured from materials used to seal PCR plates and compatible with hybridization reagents without contamination. Includes 50 Seal Strips and 1 Applicator
ProPlate® stainless steel Spring Clips are easily applied and removed and are preferred by customers using glass slides fabricated by means of a laser and which may have small micro-fractures from processing or chipped edges.
ProPlate® stainless steel Spring Clips are easily applied and removed and are preferred by customers using glass slides fabricated by means of a laser and which may have small micro-fractures from processing or chipped edges.
Organize up to 4 chambered slides into the footprint of a microtiter plate for use with automated liquid handling robots or plate washers.
Organize up to 3 chambered slides into the footprint of a microtiter plate for use with automated liquid handling robots or plate washers.
