PATH® Protein Microarray Slides

PATH® PROTEIN MICROARRAY SLIDES

PATH® Protein Microarray Slides are coated with an ultra-thin nitrocellulose film for the noncovalent, yet irreversible, binding of printed antibodies to the slide surface while maintaining antigen-binding capability. PATH® slides provide maximum reliability, sensitivity, and reproducibility of multiplex protein microarray immunoassays, in addition to reducing background fluorescence and maximizing signal-to-noise ratios.

PATH® slides provide many features of nitrocellulose in a thin film, including:

  • high signal-to-noise ratio
  • uniform spot morphology
  • 4-5 logs of dynamic range
  • excellent immunoassay reproducibility
  • extended stability and shelf life
  • individual barcoding options.

Through these benefits, users can gain up to 10X sensitivity and more reliable results across a wide range of analyte concentrations. The individual barcode options provide easy identification and tracking for improved experimental and quality control. PATH® slides can be used for a variety of assay applications:

  • sandwich immunoassays,
  • capture immunoassays,
  • protein profiling and protein characterization.

Films are less than 200nm thick and bound to a standard 75 mm x 25 mm x 1mm microscope slide with a usable surface area of 65 mm x 22 mm. PATH® film slides have an approximate 2-year shelf life in ambient storage temperature.

NOTE:  The  ‘bronze’ (brown) side of slides only is coated, and the silver ‘mirrored’ side is not. Print on the coated side only.

PATH® is a registered trademark of Courtagen Life Sciences and manufactured exclusively by Grace Bio-Labs, Inc.

Protein Microarray Substrates Selection Guides

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Property

Oncyte® Brand Nitrocellulose Thin Film Slides

Non-porous
Nitrocellulose Film*

Avid

Nova

SuperNova

PATH®

Binding capacity

Film thickness

12 µm

7 µm

12 µm

< 200 nm

Dynamic Range
(log scale fluorescence)

5-6

5-6

7+

4-5

Hydrophobicity1
(contact angle)

80°

70°

60°

70°

Preferred Detection
Methods 2

Colorimetric,
Chemiluminescence

Fluorescence

Fluorescence

Fluorescence

Applications

Antigen or antibody binding, cell or tissue lysate binding, glycoprotein and peptide binding assays

Antigen or antibody binding, glycoprotein and peptide binding assays

Antigen or antibody binding cell or tissue lysate binding glycoprotein and peptide binding assay

Antigen or antibody binding, glycoprotein and peptide binding assays

*   Exclusively from Grace Bio-Labs
1   More hydrophobic surfaces may result in reduced spot diameter, depending on the spotting buffer composition. Results may vary based on buffers, sample preparation, spotting and scanning instruments. See our ONCYTE® user guide for full discussion.
2   All detection methods are compatible with ONCYTE® Nitrocellulose Film Slides.

References

Pollard, H. B., Srivastava, M., Eidelman, O., Jozwik, C., Rothwell, S. W., Mueller, G. P., … Paweletz, C. P. (2007). Protein microarray platforms for clinical proteomics. PROTEOMICS – Clinical Applications, 1(9), 934–952. https://doi.org/10.1002/prca.200700154
 
Gyorgy, A. B., Walker, J., Wingo, D., Eidelman, O., Pollard, H. B., Molnar, A., & Agoston, D. V. (2010). Reverse phase protein microarray technology in traumatic brain injury. Journal of Neuroscience Methods, 192(1), 96–101. https://doi.org/10.1016/j.jneumeth.2010.07.029
 
Cao, Z., Partyka, K., McDonald, M., Brouhard, E., Hincapie, M., Brand, R. E., … Haab, B. B. (2013). Modulation of Glycan Detection on Specific Glycoproteins by Lectin Multimerization. Analytical Chemistry, 85(3), 1689–1698. https://doi.org/10.1021/ac302826a
 

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