PATH® Protein Microarray Slides
PATH® PROTEIN MICROARRAY SLIDES
PATH® Protein Microarray Slides are coated with an ultra-thin nitrocellulose film for the noncovalent, yet irreversible, binding of printed antibodies to the slide surface while maintaining antigen-binding capability. PATH® slides provide maximum reliability, sensitivity, and reproducibility of multiplex protein microarray immunoassays, in addition to reducing background fluorescence and maximizing signal-to-noise ratios.
PATH® slides provide many features of nitrocellulose in a thin film, including:
- high signal-to-noise ratio
- uniform spot morphology
- 4-5 logs of dynamic range
- excellent immunoassay reproducibility
- extended stability and shelf life
- individual barcoding options.
Through these benefits, users can gain up to 10X sensitivity and more reliable results across a wide range of analyte concentrations. The individual barcode options provide easy identification and tracking for improved experimental and quality control. PATH® slides can be used for a variety of assay applications:
- sandwich immunoassays,
- capture immunoassays,
- protein profiling and protein characterization.
Films are less than 200nm thick and bound to a standard 75 mm x 25 mm x 1mm microscope slide with a usable surface area of 65 mm x 22 mm. PATH® film slides have an approximate 2-year shelf life in ambient storage temperature.
NOTE: The ‘bronze’ (brown) side of slides only is coated, and the silver ‘mirrored’ side is not. Print on the coated side only.
PATH® is a registered trademark of Courtagen Life Sciences and manufactured exclusively by Grace Bio-Labs, Inc.
Protein Microarray Substrates Selection Guides
Property | Oncyte® Brand Nitrocellulose Thin Film Slides | Non-porous | ||
Binding capacity | ||||
Film thickness | 12 µm | 7 µm | 12 µm | < 200 nm |
Dynamic Range | 5-6 | 5-6 | 7+ | 4-5 |
Hydrophobicity1 | 80° | 70° | 60° | 70° |
Preferred Detection | Colorimetric, | Fluorescence | Fluorescence | Fluorescence |
Applications | Antigen or antibody binding, cell or tissue lysate binding, glycoprotein and peptide binding assays | Antigen or antibody binding, glycoprotein and peptide binding assays | Antigen or antibody binding cell or tissue lysate binding glycoprotein and peptide binding assay | Antigen or antibody binding, glycoprotein and peptide binding assays |
1 More hydrophobic surfaces may result in reduced spot diameter, depending on the spotting buffer composition. Results may vary based on buffers, sample preparation, spotting and scanning instruments. See our ONCYTE® user guide for full discussion.
2 All detection methods are compatible with ONCYTE® Nitrocellulose Film Slides.
Applications
PATH® slides offer high sensitivity for a variety of applications:
References
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