Protein Crystallization

Protein crystallization is a key assay for structural studies of proteins. The protocols for crystallization of protein are challenging due to the stringent requirement for pure samples and control of environmental conditions during the crystallization process. Vapor diffusion using hanging drop is a preferred method for obtaining quality crystals with…

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Microarray Substrates

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Grace Bio-Labs microarray surface chemistry is based on the well-known protein-binding properties of nitrocellulose. A range of different formulations have…

APPLICATIONS:

Antigen-Capture AssayAntibody Capture AssayRPPA- Reverse Phase Protein MicroarrayLaser micro-dissection RRPAEpitope-mappingBiomarker Discovery and ValidationImmunogen Discovery

Microarray Reagents

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Grace Bio-Labs microarray regents have been specifically formulated to achieve the full potential of porous nitrocellulose, accelerating experimental design and…

APPLICATIONS:

Antigen-Capture Assay Antibody Capture Assay RPPA- Reverse Phase Protein Microarray Laser micro-dissection RRPA Epitope-mapping Biomarker Discovery and Validation Immunogen Discovery

ProPlate®

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ProPlates® were specifically designed to enable automated robotic liquid handling. Two main configurations are available: The ProPlate® Microtiter Plate is comprised…

APPLICATIONS:

ProteomicsProtein MicroarraysProtein expression analysisAntibody profiling cDNA and oligonucleotide arrays

FastWells™ Reagent Barriers

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FastWells™ are sticky, flexible silicone gaskets that form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Gaskets may…

APPLICATIONS:

MicroscopyImagingFluorescence In situ Hybridization (FISH)Single-molecule fluorescence analysisImmunohistochemistry

CoverWell™ Incubation Chambers

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CoverWell™ incubation chambers are reusable, easy to apply chambers that attach without the use of adhesive.  CoverWells™ enclose a large…

APPLICATIONS:

In-situ hybridizationImmunohistochemistryDNA MicroarrayReverse Transfection Microarray

CoverWell Perfusion Chambers

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CoverWell ™ perfusion press-to-seal covers form water-tight, multiwell cell incubation or cytochemistry chambers when pressed to coverslips or microscope slides.…

APPLICATIONS:

Live-cell imagingMicroscopyImagingSingle molecule spectroscopy

HybriSlip™ Hybridization Covers

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HybriSlips™ are rigid, light-weight, thin plastic coverslips that minimize friction and facilitate uniform reagent distribution during incubation steps which require…

APPLICATIONS:

In-situ HybridizationLorem ipsum dolorIn-situ PCRImmunohistochemistryProtein MicroarrayDNA Microarray

Imaging Spacers

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Imaging spacers are ultra-thin adhesive spacers which peel-and-stick to coverglass or microscope slides to confine specimens without compression. Layer multiple…

APPLICATIONS:

ImagingMicroscopyHigh-temperature single-molecule kinetic analysisanti‐Stokes Raman scattering microscopy

SecureSeal™ Hybridization Chambers

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SecureSeal™ Hybridization Chambers are thin, silicone-gasketed chambers providing optimal surface-to-volume fluid dynamics for hybridization assays on large or multiple specimens…

APPLICATIONS:

In situ hybridizationProtein and DNA MicroarraysImmunocytochemistryRapid microfluidic prototypingFluorescence Resonance Energy Transfer (FRET)

CoverWell™ Incubation Chambers

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CoverWell™ incubation chambers are reusable, easy to apply chambers that attach without the use of adhesive.  CoverWells™ enclose a large…

APPLICATIONS:

Reverse Transfection Microarray;  DNA Microarray;  In-situ hybridization;  Immunohistochemistry; 

Silicone Isolators

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Silicone Isolators allow researchers to isolate specimens using removable hydrophobic barriers. They may be used to isolate cells grown in…

SecureSeal™ Hybridization Chambers

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SecureSeal™ Hybridization Chambers are thin, silicone-gasketed chambers providing optimal surface-to-volume fluid dynamics for hybridization assays on large or multiple specimens…

HybriWell™ Sealing System

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HybriWell™ Sealing System bonds securely to a microscope slide surface in seconds to confine small reagent volumes with samples and…

Hybridization and Incubation

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Hybridization and incubation Seals ad Chambers from Grace Bio-Labs are ideally suited for in situ-hybridization assays. The adhesive seal of…

APPLICATIONS:

In-situ hybridization MicroarraysFluorescence In situ Hybridization (FISH)FRET (Fluorescence Resonance Energy Transfer)

HybriWell™ Sealing System 8 Pack

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Chamber 8-Pack Assortment – Includes Port Seals and Applicator

Adhesive Seal Tabs

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Adhesive seal tabs complement SecureSeal™ Hybridization Chambers, HybriWell™ Sealing System and CoverWell™ Perfusion Chambers. Once the paper liner is removed,…

APPLICATIONS:

In situ hybridization ;  Protein and DNA Microarrays ;  Immunocytochemistry ;  DNA Amplification ;  Neurophysiology ;  Reaction kinetics ; 

FastWells™ Reagent Barriers

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FastWells™ are sticky, flexible silicone gaskets that form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Gaskets may…

FlexWell™ 4 Slide Tray

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Multiple slides and FlexWells™ may be inserted into a reusable FlexWell™ tray, organizing wells into the footprint of a SBS…

APPLICATIONS:

Protein MicroarrayHybridizationIncubation

FlexWell™ Incubation Chambers

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FlexWell™ incubation chamber silicone gaskets form wells on slides using clean release adhesive to isolate up to 16 specimens per…

APPLICATIONS:

Protein MicroarrayHybridizationIncubation

FlexWell™ Seal Strips

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To prevent evaporation apply seal strips using the plastic applicator to the top surface of the chamber. Seal strips remove cleanly…

APPLICATIONS:

Microarrays using nitrocellulose coatedHybridizationIncubation

HybriSlip™ Hybridization Covers

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HybriSlips™ are rigid, light-weight, thin plastic coverslips that minimize friction and facilitate uniform reagent distribution during incubation steps which require…

ProPlates®

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ProPlates® were specifically designed to enable automated robotic liquid handling. Two main configurations are available: The ProPlate® Microtiter Plate is comprised…

APPLICATIONS:

ProteomicsProtein MicroarraysProtein expression analysis;  Antibody profiling ;  cDNA and oligonucleotide arrays; 

Silicone Isolators™ Sheet Material

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Silicone isolator™ sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. Where additional sealing is…

APPLICATIONS:

Protein and DNA arrays ;  Immunohistochemistry;  Fluorescence In situ Hybridization (FISH) ;  Biopolymers and hydrogel formulation ;  Cryogenic-transmission electron microscopy (Cryo-TEM) ;  Microwave crystallization ;  Ultra-small-angle X-ray scattering (USAXS) ;  Tissue ingeneering;  Live cell lithography” (LCL); 

Imaging Spacers

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Imaging spacers are ultra-thin adhesive spacers which peel-and-stick to coverglass or microscope slides to confine specimens without compression. Layer multiple…

APPLICATIONS:

Imaging;  Microscopy;  High-temperature single-molecule kinetic analysis;  Anti‐Stokes Raman scattering microscopy; 

CoverWell™ Imaging Chambers

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CoverWell ™ imaging chambers are designed to stabilize and support thick and free-floating specimens for confocal microscopy and imaging applications.…

APPLICATIONS:

Confocal microscopy Imaging Tissue and Cell staining ;  High Resolution Microscopy ;  Live-cell imaging ; 

CoverWell™ Perfusion Chambers

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CoverWell ™ perfusion press-to-seal covers form water-tight, multiwell cell incubation or cytochemistry chambers when pressed to coverslips or microscope slides.…

APPLICATIONS:

Single molecule spectroscopy Live-cell imaging Microscoscopy

FastWells™ Reagent Barriers

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FastWells™ are sticky, flexible silicone gaskets that form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Gaskets may…

APPLICATIONS:

Microscopy Fluorescence In situ Hybridization (FISH) Single-molecule fluorescence analysis ;  Immunohistochemistry ; 

MultiSlip™ Coverglass Inserts

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MutliSlip™ inserts with 8 (18mm x 18mm) or 15 (12mm x 12mm) No. 1.5 German glass coverglass per insert are…

APPLICATIONS:

High resolution microscopy Fluorescent imaging Immunohistochemistry ;  Cell Culture; 

SecureSeal™ Adhesive Sheets

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These adhesive sheets are made using the same SecureSeal™ adhesive as is used to make HybriWell™ and SecureSeal™ Incubation Chambers.  Thin,…

APPLICATIONS:

Imaging ;  Tissue and Cell staining ;  High Resolution Microscopy; 

SecureSlip™ Silicone Supported Coverglass

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SecureSlip™ Silicone Supported Coverglass is affixed to a thin microscopically transparent silicone base which secures it to culture vessels by…

Imaging

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Imaging seals and chambers from Grace Bio-Labs offer a selection of tools for cell/tissue staining for high quality results in…

APPLICATIONS:

Tissue and Cell stainingHigh Resolution MicroscopyLive-cell imaging

Adhesive Seal Tabs

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Adhesive seal tabs complement SecureSeal™ Hybridization Chambers, HybriWell™ Sealing System and CoverWell™ Perfusion Chambers. Once the paper liner is removed,…

APPLICATIONS:

In situ hybridization ;  Protein and DNA Microarrays;  Immunocytochemistry ;  DNA Amplification ;  Neurophysiology ;  Reaction kinetics; 

CultureWell™ Coverglass Removal Tool

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CS16 CultureWell™ removable chambered coverglass is a 16-well chambered coverglass cell culture vessel, with 2 x 8 format with standard…

APPLICATIONS:

Cell culture Fluorescence imaging

CultureWell™ Microplate Adapter Tray

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CultureWell™ Microplate Adapter Tray holds up to 4 CultureWell™ 16 Chambers for higher throughput

CultureWell™ MultiWell Chambered Coverslips

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CultureWell™ chambered coverglass products consist of removable and reusable, non-cytotoxic silicone gaskets secured to number 1.5 German coverglass. Chambered coverglass…

APPLICATIONS:

Cell Culture Fluorescence applications In-situ hybridization Immunostaining

CS16-CultureWell™ Removable Chambered Coverglass

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CS16 CultureWell™ removable chambered coverglass is a 16-well chambered coverglass cell culture vessel, with 2 x 8 format with standard…

APPLICATIONS:

Cell CultureFluorescence applicationsIn-situ hybridizationImmunostaining

CultureWell™ Coverglass Inserts

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Each CultureWell™ coverglass insert is comprised of four chambered coverglass, assembled in a disposable frame placed in a standard 86mm…

APPLICATIONS:

High resolution microscopy Fluorescent imaging Immunohistochemistry

CultureWell™ Reusable Gaskets

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Gaskets are ideal for forming wells on glass microscope slides or in polystyrene dishes. Gaskets are non-sterile and may be…

APPLICATIONS:

Cell CultureHigh resolution microscopyFluorescent imaging Immunohistochemistry

CultureWell™ Silicone Sheet Material

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CultureWell™ clear silicone sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. They may be…

APPLICATIONS:

Cell CultureHigh resolution microscopy Fluorescent imagingImmunohistochemistry

MultiSlip™ Coverglass Inserts

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MutliSlip™ inserts with 8 (18mm x 18mm) or 15 (12mm x 12mm) No. 1.5 German glass coverglass per insert are…

APPLICATIONS:

Cell CultureFluorescent imaging Immunohistochemistry

SecureSlip™ Silicone Supported Coverglass

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SecureSlip™ Silicone Supported Coverglass is affixed to a thin microscopically transparent silicone base which secures it to culture vessels by…

APPLICATIONS:

Cell CultureImmunofluorescence assayMicroscopy

CultureWell™ ChamberSLIP 16, Non-Removable Chambered Coverglass

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CultureWell™ NON Removable Chambered Coverglass, 16 Well, No. 1.5 German borosilicate Coverglass. Product consists of cell culture vessels, with a…

APPLICATIONS:

Cell Culture Fluorescence applicationsSmall volume incubation Immunostaining

Silicone Wound Splints

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Wound splints are constructed of silicone and include suture sites for increased precision in affixing on or within an animal…

Silicone Isolator Sheet Material

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Silicone isolator™ sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. Where additional sealing is…

APPLICATIONS:

Protein and DNA arrays ;  Immunohistochemistry ;  Fluorescence In-situ Hybridization (FISH) ;  Biopolymers and hydrogel formulation;  Cryogenic-transmission electron microscopy (Cryo-TEM) ;  X-ray scattering ;  Microwave crystallization ;  Ultra-small-angle X-ray scattering (USAXS) ;  Tissue engineering Live cell lithography (LCL); 

CultureWell Silicone Sheet Material

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CultureWell™ clear silicone sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. They may be…

APPLICATIONS:

Lorem Ipsum ;  Lorem Ipsum;  Lorem Ipsum; 

ONCYTE® Film slides

ONCYTE® FILM SLIDES

ONCYTE ® Porous nitrocellulose Film slides are comprised of a microporous nitrocellulose film cast on a variety of solid surfaces. They offer numerous advantages over functionalized  surface chemistries:

  • An increased available surface translates into high protein binding capacity.
  • Fluorescence Signal Amplification via the Tyndall effect for enhanced sensitivity.
  • Broad dynamic range and low detection limit

SEM image of ONCYTE® Porous nitrocellulose film. The small pore supplies a very large surface
area which contributes to its high binding capacity. The pore structures in this formulation promote controlled and uniform wetting, thus making it ideal for microarray deposition.

Tyndal Effect. When excitation light strikes the surface of the PNC, tiny voids in the film scatter the incident light, causing many of the photons to reflect back onto fluorophores, thus increasing the probability that the excitation light will be absorbed by a fluorophore. Thus the effective “extinction coefficient” is greatly increased.

Detection of Antibody Binding Capacity on Blocked Substrates. Data represent mean fluorescent intensities from serial dilutions of IgG-TRITC spotted in duplicates on multiple array slides (n=4) after extensive washing and detected with 532 nm excitation. Biotechniques: 2013 Vol 54, No 4: pg 223-225

ONCYTE® film slides are manufactured to the highest standards and quality tested, they provide excellent reliability and reproducible. There are many existing pad configurations available, and Grace has the ability to customize film patterns to meet the needs of customers. In addition, Grace offers strong R&D support for new applications and quality support for variance control of key performance indicators.

Protein Microarray Substrates Selection Guide

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Property

Oncyte® Brand Nitrocellulose Thin Film Slides

Non-porous
Nitrocellulose Film*

Avid

Nova

SuperNova

Path®

Binding capacity

Film thickness

12 µm

7 µm

12 µm

< 200 nm

Dynamic Range
(log scale fluorescence)

5-6

5-6

7+

4-5

Hydrophobicity1
(may affect spot size)

Preferred Detection
Methods 2

Colorimetric,
Chemiluminescence

Fluorescence

Fluorescence

Fluorescence

Applications

Antigen or antibody binding, cell or tissue lysate binding, glycoprotein and peptide binding assays

Antigen or antibody binding, glycoprotein and peptide binding assays

Antigen or antibody binding cell or tissue lysate binding glycoprotein and peptide binding assay

Antigen or antibody binding, glycoprotein and peptide binding assays

*   Exclusively from Grace Bio-Labs
1   More hydrophobic surfaces may result in reduced spot diameter, depending on the spotting buffer composition. Results may vary based on buffers, sample preparation, spotting and scanning instruments. See our ONCYTE® user guide for full discussion.
2   All detection methods are compatible with ONCYTE® Nitrocellulose Film Slides.

Videos

Reverse Phase Protein Arrays

ONCYTE Film Slide Overview

2013 Microarray World Congress Presentation By Grace Bio-Labs

References

Pin, Elisa et al. 2016. “A Pilot Study Exploring the Molecular Architecture of the Tumor Microenvironment in Human Prostate Cancer Using Laser Capture Microdissection and Reverse Phase Protein Microarray.” Molecular Oncology 10(10):1585–94. Retrieved March 17, 2017 (http://www.sciencedirect.com/science/article/pii/S1574789116301077).

Wright, David K. et al. 2016. “Behavioral, Blood, and Magnetic Resonance Imaging Biomarkers of Experimental Mild Traumatic Brain Injury.” Scientific Reports 6. Retrieved March 17, 2017 (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4923906/).

Kramer, Jill M. et al. 2016. “Analysis of IgM Antibody Production and Repertoire in a Mouse Model of Sjögren’s Syndrome.” Journal of Leukocyte Biology 99(2):321–31. Retrieved March 17, 2017 (http://www.jleukbio.org/content/99/2/321).

See more references for ONCYTE Film slides here

Applications

Oncyte List of Applications

  • RPPA- reverse phase protein microarrays
  • Laser microdissection- RRPA
  • Antigen-capture assay
  • Antibody capture assay
  • Biomarker Discovery and Validation
  • Nanotoxicology
  • Serological Assay
  • Immunogen Discovery

FAQ

Q. Which concentrations of antibodies or proteins should be printed on the ONCYTE® Film Slides?

A. For purified proteins a concentration of about 0.05-1 mg/ml is optimal for most applications, the upper end of this range is recommended for antibodies.

Q. Should printing be performed at low temperatures?

A. No, printing may be performed at room temperature as long as humidity is maintained at around 50-55% to avoid sample evaporation during the process.

Q. My protein sample contains urea, is this going to affect protein binding to ONCYTE® Film Slides?

A. No. Samples containing urea are routinely printed on ONCYTE® Film Slides. Slides are chemically resistant to reagents typically used in most assays for cell analysis including 50% formamide. Film slides are not recommended for use with acetone, ethanol, and methanol. Isopropanol and butanol are common substitutes. DMSO could negatively affect nitrocellulose too, therefore its concentration should not exceed 5%.

Q. Can traditional western blot blocking buffers be used for blocking protein microarrays?

A. The composition of the blocking buffer should be optimized for each experimental setting. Colorimetric and chemiluminescent detections are compatible with the blocking buffers commonly used for western blot, such as PBS or TBS containing 1-5% non-fat milk. Fluorescent detection, on the other hand, can be hampered by background fluorescence typically associated with protein–based blocking buffers. Super G Protein array blocking buffer has been optimized for fluorescent assays and produces a very high signal to noise ratio, minimizing the background fluorescence.

Q. How do I image my microarray results?

A. ONCYTE® Film Slides are compatible virtually with all detection systems: isotopic, chemiluminescent, chromogenic, and fluorescent detections are all viable options.

Q. How should printed ONCYTE® Film Slides be stored?

A. Non-printed slides should be stored at room temperature in their original packaging. Printed slides can be stored at 4°C for short-term storage or at -20°C for long-term storage. An overnight incubation at 4°C is recommended after printing in order to maximize protein binding before use.

Q. What kind of controls should be included in the microarray analysis?

A. IgG pre-labeled with a fluorophore should be spotted on the array to check for proper protein binding. Buffer-only spots can be used for background subtraction, as well as secondary antibody-only stained film slides. Replicates should also be spotted on the array for each protein.

Q. Can ONCYTE® Film Slides be ‘stripped’ and re-used?

A. No. It is not advisable to re-use ONCYTE® Film Slides.

10X PBST Wash Buffer

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Blocking buffers, spotting buffers and sample preparation kits for consistent results and improved sensitivity in protein microarrays.

ProPlate® Microtiter Plates

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Patented ProPlate® MTP technology for high throughput robotic processing of microarrays allows you to combine your content arrayed substrate to a disposable ANSI-SLAS -compliant microtiter plate.

ProPlate® Multi-Array Slide System

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ProPlate® re-usable, multi-well chambers form removable, leak-proof wells on virtually any slide surface without the use of adhesive. Designs feature ANSI-SLAS compliant well spacing to facilitate multichannel pipetting. Multiple slides insert easily into frames having a standard microtiter plate footprint for automated processing.

ProPlate® Multi-Well Chambers

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ProPlate® re-usable, multi-well chambers are available in a wide variety of formats to fit standard 1 x 25 x 75 mm glass microscope slide substrates. Chambers having a depth of approximately 7.5 mm provide a generous surface to volume ratio to facilitate mixing and washing steps.

GBL Protein Array Buffer

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GBL Protein Arraying Buffer is a non-denaturing microarray spotting buffer designed to work with our ONCYTE® porous nitrocellulose film slides.

Protein Array Assay System

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The Protein Array Assay System includes the key reagents necessary for maximal use of our ONCYTE® nitrocellulose film slides and can be used in various protein microarray applications such as antibody and antigen capture arrays.

QBlock™ Protein Microarray Blocking Buffer

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QBlock™ Protein Microarray Blocking Buffer is a non-protein based reagent designed to block non-specific protein binding on porous nitrocellulose substrates.This blocker was formulated for maximizing assay signal while minimizing background from assays utilizing Quantum Nanoparticles (QNPs) for detection, and is also compatible with other assay types using organic fluors (e.g. Alexa Fluor dyes, Li-Cor dyes) or colorimetric endpoint detection (e.g DAB) after enzymatic amplification. It is supplied as a 1X solution, ready to use out of the bottle, for microarray applications.

Reverse Phase Protein Array (RPPA) Assay System

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The RPPA Assay System from Grace Bio-Labs was designed to provide users with an effective and easy-to-implement introduction to RPPA technology.  The standardized RPPA reagents provided in this kit offer an effective system for generating high-quality data from RPPA assays. In addition, use of this kit can contribute to consistent results between assays, laboratories, and institutions.

Super G™ Blocking Buffer

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Super G™ Blocking Buffer optimizes the use of nitrocellulose for protein assays using fluorescence detection. Results with Super G™ show significant reduction in fluorescence background while maintaining the high protein-binding capacity of porous nitrocellulose. Purified, protein-free solution has high binding efficiency for rapid and comprehensive blocking of non-specific protein.

Super G™ Plus Protein Preservative

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Super G™ Plus Preservation Buffer delivers highly efficient blocking of non-specific protein binding on polymer films, with additional components optimized for long-term protein preservation.  This formulation improves assay performance for various proteins stored for extended periods after immobilization on polymer films.

PATH® Protein Microarray Slides

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PATH® Protein Microarray Slides are coated with an ultra-thin nitrocellulose film for the noncovalent, yet irreversible, binding of printed antibodies to the slide surface while maintaining antigen binding capability. PATH® slides provide maximum reliability, sensitivity, and reproducibility of multiplex protein microarray immunoassays, in addition to reducing background fluorescence and maximizing signal-to-noise ratios.