PROTEIN MICROARRAY SUBSTRATES
Grace Bio-Labs microarray surface chemistry is based on the well-known protein-binding properties of nitrocellulose. Grace Bio-Labs selection of microarray substrates comprises:
- PATH®: Non-porous nitrocellulose film slides
- ONCYTE® NOVA, AVID, SUPERNOVA: porous nitrocellulose Film slides
Both feature:
- preservation of native protein conformation
- high signal to noise ratio
- increased dynamic range for protein binding
- multiplex capability
Furthermore, they are compatible with virtually all detection systems (colorimetric, chemiluminescent, fluorescent, near-IR).
PATH® is a registered trademark of Courtagen Life Sciences and manufactured exclusively by Grace Bio-Labs, Inc.
Property | Oncyte® Brand Nitrocellulose Thin Film Slides | Non-porous | ||
Binding capacity | ||||
Film thickness | 12 µm | 7 µm | 12 µm | < 200 nm |
Dynamic Range | 5-6 | 5-6 | 7+ | 4-5 |
Hydrophobicity1 | 80° | 70° | 60° | 70° |
Preferred Detection | Colorimetric, | Fluorescence | Fluorescence | Fluorescence |
Applications | Antigen or antibody binding, cell or tissue lysate binding, glycoprotein and peptide binding assays | Antigen or antibody binding, glycoprotein and peptide binding assays | Antigen or antibody binding cell or tissue lysate binding glycoprotein and peptide binding assay | Antigen or antibody binding, glycoprotein and peptide binding assays |
1 More hydrophobic surfaces may result in reduced spot diameter, depending on the spotting buffer composition. Results may vary based on buffers, sample preparation, spotting and scanning instruments. See our ONCYTE® user guide for full discussion.
2 All detection methods are compatible with ONCYTE® Nitrocellulose Film Slides.
ONCYTE ® Porous nitrocellulose Film slides are comprised of a microporous nitrocellulose film cast on a variety of solid surfaces. They offer numerous advantages over functionalized surface chemistries
PATH® Protein Microarray Slides are coated with an ultra-thin nitrocellulose film for the noncovalent, yet irreversible, binding of printed antibodies to the slide surface while maintaining antigen binding capability. PATH® slides provide maximum reliability, sensitivity, and reproducibility of multiplex protein microarray immunoassays, in addition to reducing background fluorescence and maximizing signal-to-noise ratios.
