PROTEIN MICROARRAY SUBSTRATES



Grace Bio-Labs microarray surface chemistry is based on the well-known protein-binding properties of nitrocellulose. Grace Bio-Labs selection of microarray substrates comprises:

Both feature:

  • preservation of native protein conformation
  • high signal to noise ratio
  • increased dynamic range for protein binding
  • multiplex capability

Furthermore, they are compatible with virtually all detection systems (colorimetric, chemiluminescent, fluorescent, near-IR).

PATH® is a registered trademark of Courtagen Life Sciences and manufactured exclusively by Grace Bio-Labs, Inc.

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Property

Oncyte® Brand Nitrocellulose Thin Film Slides

Non-porous
Nitrocellulose Film*

Avid

Nova

SuperNova

PATH®

Binding capacity

Film thickness

12 µm

7 µm

12 µm

< 200 nm

Dynamic Range
(log scale fluorescence)

5-6

5-6

7+

4-5

Hydrophobicity1
(contact angle)

80°

70°

60°

70°

Preferred Detection
Methods 2

Colorimetric,
Chemiluminescence

Fluorescence

Fluorescence

Fluorescence

Applications

Antigen or antibody binding, cell or tissue lysate binding, glycoprotein and peptide binding assays

Antigen or antibody binding, glycoprotein and peptide binding assays

Antigen or antibody binding cell or tissue lysate binding glycoprotein and peptide binding assay

Antigen or antibody binding, glycoprotein and peptide binding assays

*   Exclusively from Grace Bio-Labs
1   More hydrophobic surfaces may result in reduced spot diameter, depending on the spotting buffer composition. Results may vary based on buffers, sample preparation, spotting and scanning instruments. See our ONCYTE® user guide for full discussion.
2   All detection methods are compatible with ONCYTE® Nitrocellulose Film Slides.

APPLICATIONS:

  • Antigen-Capture Assay
  • Antibody Capture Assay
  • Coronavirus Antigen Array
  • Epitope-mapping
  • Biomarker Discovery and Validation
  • Immunogen Discovery