SuperG™ Plus Protein Preservative
SUPER G™ PLUS PROTEIN PRESERVATIVE
SuperG™ Plus Preservation Buffer delivers highly efficient blocking of non-specific protein binding on polymer films, with additional components optimized for long-term protein preservation. This formulation improves assay performance for various proteins stored for extended periods after immobilization on polymer films.
In the above example cytokine antigenicity was monitored over time, with and without SUPER G™ PLUS PROTEIN PRESERVATIVE. Without effective preservation 75% of printed cytokines lost antigenicity in less than 200 days. With SUPER G™ PLUS 100 % of antigenicity was retained over the course of 1 year.
Applications
- RPPA- reverse phase protein microarrays
- Laser microdissection- RRPA
- Antigen-capture assay
- Antibody capture assay
- Biomarker Discovery and Validation
- Nanotoxicology
- Serological Assay
- Immunogen Discovery
References
Anderson, Troy, Julia Wulfkuhle, Emanuel Petricoin, and Raimond L Winslow. "High Resolution Mapping of the Cardiac Transmural Proteome Using Reverse Phase Protein Microarrays." Molecular & Cellular Proteomics : MCP 10, no. 7 (2011): M111.008037. (https://www.ncbi.nlm.nih.gov/pubmed/21490165)
Ecker, Stephanie M., Joshua C. Hines, Scott M. Pfahler, and Bert M. Glaser. "Aqueous Cytokine and Growth Factor Levels Do Not Reliably Reflect Those Levels Found in the Vitreous." Molecular Vision 17 (2011): 2856-863. (https://www.ncbi.nlm.nih.gov/pubmed/22128233)
Kim, Geoffrey, Ben Davidson, Ryan Henning, Junbai Wang, Minshu Yu, Christina Annunziata, Thea Hetland, and Elise C. Kohn. "Adhesion Molecule Protein Signature in Ovarian Cancer Effusions Is Prognostic of Patient Outcome." Cancer 118, no. 6 (2012): 1543-553.(https://www.ncbi.nlm.nih.gov/pubmed/term=Adhesion+Molecule+Protein+Signature+in+Ovarian+Cancer+Effusions+Is+Prognostic+of+Patient+Outcome)
Patented ProPlate® MTP technology for high throughput robotic processing of microarrays allows you to combine your content arrayed substrate to a disposable ANSI-SLAS -compliant microtiter plate.
ProPlate® re-usable, multi-well chambers form removable, leak-proof wells on virtually any slide surface without the use of adhesive. Designs feature ANSI-SLAS compliant well spacing to facilitate multichannel pipetting. Multiple slides insert easily into frames having a standard microtiter plate footprint for automated processing.
GBL Protein Arraying Buffer is a non-denaturing microarray spotting buffer designed to work with our ONCYTE® porous nitrocellulose film slides.
QBlock™ Protein Microarray Blocking Buffer is a non-protein based reagent designed to block non-specific protein binding on porous nitrocellulose substrates.This blocker was formulated for maximizing assay signal while minimizing background from assays utilizing Quantum Nanoparticles (QNPs) for detection, and is also compatible with other assay types using organic fluors (e.g. Alexa Fluor dyes, Li-Cor dyes) or colorimetric endpoint detection (e.g DAB) after enzymatic amplification. It is supplied as a 1X solution, ready to use out of the bottle, for microarray applications.
SuperG™ Blocking Buffer optimizes the use of nitrocellulose for protein assays using fluorescence detection. Results with Super G™ show significant reduction in fluorescence background while maintaining the high protein-binding capacity of porous nitrocellulose. Purified, protein-free solution has high binding efficiency for rapid and comprehensive blocking of non-specific protein.
Grace Bio-Labs microarray surface chemistry is based on the well-known protein-binding properties of nitrocellulose. A range of different formulations have been developed to provide optimal solutions for all protein microarray applications.
