SuperG™ Plus Protein Preservative

SUPER G™ PLUS PROTEIN PRESERVATIVE

SuperG™ Plus Preservation Buffer delivers highly efficient blocking of non-specific protein binding on polymer films, with additional components optimized for long-term protein preservation.  This formulation improves assay performance for various proteins stored for extended periods after immobilization on polymer films.

Storage was under accelerated conditions at 38°C for up to 35 days equivalent to 1 year storage at 4°C. Controls were assayed immediately after preservation incubations. Assays were performed with individual anti-antigen antibodies and detected by fluorescence at 532nm (N=4 arrays).
Improved detection of Printed Antigenic Determinants with Optimized Preservation Reagents. Storage was under accelerated conditions at 38°C for up to 35 days equivalent to 1 year storage at 4°C. Controls were assayed immediately after preservation incubation. Assays were performed with individual anti-antigen antibodies and detected by fluorescence at 532 nm (N=4 arrays).

In the above example cytokine antigenicity was monitored over time, with  and without SUPER G™ PLUS PROTEIN PRESERVATIVE. Without effective preservation 75% of printed cytokines lost antigenicity in less than 200 days. With SUPER GPLUS 100 % of antigenicity was retained over the course of 1 year.

Applications

  • RPPA- reverse phase protein microarrays
  • Laser microdissection- RRPA
  • Antigen-capture assay
  • Antibody capture assay
  • Biomarker Discovery and Validation
  • Nanotoxicology
  • Serological Assay
  • Immunogen Discovery

References

Anderson, Troy, Julia Wulfkuhle, Emanuel Petricoin, and Raimond L Winslow. "High Resolution Mapping of the Cardiac Transmural Proteome Using Reverse Phase Protein Microarrays." Molecular & Cellular Proteomics : MCP 10, no. 7 (2011): M111.008037. (https://www.ncbi.nlm.nih.gov/pubmed/21490165)

Ecker, Stephanie M., Joshua C. Hines, Scott M. Pfahler, and Bert M. Glaser. "Aqueous Cytokine and Growth Factor Levels Do Not Reliably Reflect Those Levels Found in the Vitreous." Molecular Vision 17 (2011): 2856-863. (https://www.ncbi.nlm.nih.gov/pubmed/22128233)

Kim, Geoffrey, Ben Davidson, Ryan Henning, Junbai Wang, Minshu Yu, Christina Annunziata, Thea Hetland, and Elise C. Kohn. "Adhesion Molecule Protein Signature in Ovarian Cancer Effusions Is Prognostic of Patient Outcome." Cancer 118, no. 6 (2012): 1543-553.(https://www.ncbi.nlm.nih.gov/pubmed/term=Adhesion+Molecule+Protein+Signature+in+Ovarian+Cancer+Effusions+Is+Prognostic+of+Patient+Outcome)