SuperG™ Blocking Buffer
SUPER G™ BLOCKING BUFFER
SuperG™ Blocking Buffer optimizes the use of nitrocellulose for protein assays using fluorescence detection. Results with SuperG™ show significant reduction in fluorescence background while maintaining the high protein-binding capacity of porous nitrocellulose. Purified, protein-free solution has high binding efficiency for rapid and comprehensive blocking of non-specific protein.
Background fluorescence using SuperG™ Blocking Reagent is 3- to 10-fold lower at 532 nm and up to 6-fold lower at 635 nm. Signal-to-Noise is 4- to 10-fold higher at 532 nm and up to 4.5-fold higher at 635 nm. Data presented are from SuperNOVA slides blocked with SuperG™ or other protein- and non-protein-based blockers followed by sandwich assays for IL-1a, IL-1b, IL6, TNF b, and INFg. Data are the average signal-to-noise for the 5 assays and are presented relative to blocking with a common blocker (PBS with 0.1% Tween-20). Typical blocking results with SuperG™ show reduced background with use of SuperG™ compared to PBST.
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Safety Data Sheet
- RPPA- reverse phase protein microarrays
- Laser microdissection- RRPA
- Antigen-capture assay
- Antibody capture assay
- Biomarker Discovery and Validation
- Serological Assay
- Immunogen Discovery
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Mordmüller, B., Surat, G., Lagler, H., Chakravarty, S., Ishizuka, A. S., Lalremruata, A., … Kremsner, P. G. (2017). Sterile protection against human malaria by chemoattenuated PfSPZ vaccine. Nature, 542(7642), 445–449. (https://www.nature.com/nature/journal/v542/n7642/full/nature21060.html)
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Q Which buffer should Super G blocking buffer be diluted in and which dilution should be used?
A Super G blocking buffer may be diluted with PBS. 0.5 to 0.1 x is the recommended dilution range.
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