SuperG™ Blocking Buffer
SUPER G™ BLOCKING BUFFER
SuperG™ Blocking Buffer optimizes the use of nitrocellulose for protein assays using fluorescence detection. Results with SuperG™ show significant reduction in fluorescence background while maintaining the high protein-binding capacity of porous nitrocellulose. Purified, protein-free solution has high binding efficiency for rapid and comprehensive blocking of non-specific protein.
Background fluorescence using SuperG™ Blocking Reagent is 3- to 10-fold lower at 532 nm and up to 6-fold lower at 635 nm. Signal-to-Noise is 4- to 10-fold higher at 532 nm and up to 4.5-fold higher at 635 nm. Data presented are from SuperNOVA slides blocked with SuperG™ or other protein- and non-protein-based blockers followed by sandwich assays for IL-1a, IL-1b, IL6, TNF b, and INFg. Data are the average signal-to-noise for the 5 assays and are presented relative to blocking with a common blocker (PBS with 0.1% Tween-20). Typical blocking results with SuperG™ show reduced background with use of SuperG™ compared to PBST.
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Applications
- RPPA- reverse phase protein microarrays
- Laser microdissection- RRPA
- Antigen-capture assay
- Antibody capture assay
- Biomarker Discovery and Validation
- Nanotoxicology
- Serological Assay
- Immunogen Discovery
References
Partolina, M., Thoms, H. C., MacLeod, K. G., Rodriguez-Blanco, G., Clarke, M. N., Venkatasubramani, A. V., … Kagansky, A. (2017). Global histone modification fingerprinting in human cells using epigenetic reverse phase protein array. Cell Death Discovery, 3, 16077. (https://www.nature.com/articles/cddiscovery201677)
Mordmüller, B., Surat, G., Lagler, H., Chakravarty, S., Ishizuka, A. S., Lalremruata, A., … Kremsner, P. G. (2017). Sterile protection against human malaria by chemoattenuated PfSPZ vaccine. Nature, 542(7642), 445–449. (https://www.nature.com/nature/journal/v542/n7642/full/nature21060.html)
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FAQ
Q Which buffer should Super G blocking buffer be diluted in and which dilution should be used?
A Super G blocking buffer may be diluted with PBS. 0.5 to 0.1 x is the recommended dilution range.
GBL Protein Arraying Buffer is a non-denaturing microarray spotting buffer designed to work with our ONCYTE® porous nitrocellulose film slides.
QBlock™ Protein Microarray Blocking Buffer is a non-protein based reagent designed to block non-specific protein binding on porous nitrocellulose substrates.This blocker was formulated for maximizing assay signal while minimizing background from assays utilizing Quantum Nanoparticles (QNPs) for detection, and is also compatible with other assay types using organic fluors (e.g. Alexa Fluor dyes, Li-Cor dyes) or colorimetric endpoint detection (e.g DAB) after enzymatic amplification. It is supplied as a 1X solution, ready to use out of the bottle, for microarray applications.
Super G™ Plus Preservation Buffer delivers highly efficient blocking of non-specific protein binding on polymer films, with additional components optimized for long-term protein preservation. This formulation improves assay performance for various proteins stored for extended periods after immobilization on polymer films.
