SuperG™ Blocking Buffer

SUPER G™ BLOCKING BUFFER

SuperG™ Blocking Buffer optimizes the use of nitrocellulose for protein assays using fluorescence detection. Results with SuperG™ show significant reduction in fluorescence background while maintaining the high protein-binding capacity of porous nitrocellulose. Purified, protein-free solution has high binding efficiency for rapid and comprehensive blocking of non-specific protein.

 

SuperNOVA Film-Slides were assayed for IL-1, IL-1, IL6, TNF, and INF in a multiplexed antibody capture assay. And detected with TRITC- or Alexa647-labeled secondary antibodies. Data are the background fluorescent intensities (Mean ± S.D.) for N=4 slides.
Super G blocking buffer improves assay output by minimizing the background.

Background fluorescence using SuperG™ Blocking Reagent is 3- to 10-fold lower at 532 nm and up to 6-fold lower at 635 nm. Signal-to-Noise is 4- to 10-fold higher at 532 nm and up to 4.5-fold higher at 635 nm. Data presented are from SuperNOVA slides blocked with SuperG™ or other protein- and non-protein-based blockers followed by sandwich assays for IL-1a, IL-1b, IL6, TNF b, and INFg.  Data are the average signal-to-noise for the 5 assays and are presented relative to blocking with a common blocker (PBS with 0.1% Tween-20). Typical blocking results with SuperG™ show reduced background with use of SuperG™ compared to PBST.

 

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Technical Resources

Instructions

Instructions for Use - SuperG Blocking Buffer

Safety Data Sheet

SuperG Blocking Buffer

Applications

  • RPPA- reverse phase protein microarrays
  • Laser microdissection- RRPA
  • Antigen-capture assay
  • Antibody capture assay
  • Biomarker Discovery and Validation
  • Nanotoxicology
  • Serological Assay
  • Immunogen Discovery

References

Partolina, M., Thoms, H. C., MacLeod, K. G., Rodriguez-Blanco, G., Clarke, M. N., Venkatasubramani, A. V., … Kagansky, A. (2017). Global histone modification fingerprinting in human cells using epigenetic reverse phase protein array. Cell Death Discovery, 3, 16077. (https://www.nature.com/articles/cddiscovery201677)

Mordmüller, B., Surat, G., Lagler, H., Chakravarty, S., Ishizuka, A. S., Lalremruata, A., … Kremsner, P. G. (2017). Sterile protection against human malaria by chemoattenuated PfSPZ vaccine. Nature, 542(7642), 445–449. (https://www.nature.com/nature/journal/v542/n7642/full/nature21060.html)

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FAQ

Q Which buffer should Super G blocking buffer be diluted in and which dilution should be used?

A Super G  blocking buffer may be diluted with PBS.  0.5 to 0.1 x is the recommended dilution range.