Protein Array Assay System
PROTEIN ARRAY ASSAY SYSTEM
The Protein Array Assay System includes the key reagents necessary for maximal use of our ONCYTE® nitrocellulose film slides and can be used in various protein microarray applications such as antibody and antigen capture arrays.
Kit Contents Include:
User Guide Protein Array Assay System
Quick Start Guide Protein Array Assay System
User Guide ONCYTE Protein Microarrays
- Protein Microarray
- Antigen Capture Assay
- Antibody Capture Assay
Anderson, Troy, Julia Wulfkuhle, Emanuel Petricoin, and Raimond L Winslow. "High Resolution Mapping of the Cardiac Transmural Proteome Using Reverse Phase Protein Microarrays." Molecular & Cellular Proteomics : MCP 10, no. 7 (2011): M111.008037. (https://www.ncbi.nlm.nih.gov/pubmed/21490165)
Ecker, Stephanie M., Joshua C. Hines, Scott M. Pfahler, and Bert M. Glaser. "Aqueous Cytokine and Growth Factor Levels Do Not Reliably Reflect Those Levels Found in the Vitreous." Molecular Vision 17 (2011): 2856-863 (https://www.ncbi.nlm.nih.gov/pubmed/22128233)
Kim, Geoffrey, Ben Davidson, Ryan Henning, Junbai Wang, Minshu Yu, Christina Annunziata, Thea Hetland, and Elise C. Kohn. "Adhesion Molecule Protein Signature in Ovarian Cancer Effusions Is Prognostic of Patient Outcome." Cancer 118, no. 6 (2012): 1543-553. (https://www.ncbi.nlm.nih.gov/pubmed/22009736)
GBL Protein Array BufferSEARCH
GBL Protein Arraying Buffer is a non-denaturing microarray spotting buffer designed to work with our ONCYTE® porous nitrocellulose film slides.
QBlock™ Protein Microarray Blocking BufferSEARCH
QBlock™ Protein Microarray Blocking Buffer is a non-protein based reagent designed to block non-specific protein binding on porous nitrocellulose substrates.This blocker was formulated for maximizing assay signal while minimizing background from assays utilizing Quantum Nanoparticles (QNPs) for detection, and is also compatible with other assay types using organic fluors (e.g. Alexa Fluor dyes, Li-Cor dyes) or colorimetric endpoint detection (e.g DAB) after enzymatic amplification. It is supplied as a 1X solution, ready to use out of the bottle, for microarray applications.
Reverse Phase Protein Array (RPPA) Assay SystemSEARCH
The RPPA Assay System from Grace Bio-Labs was designed to provide users with an effective and easy-to-implement introduction to RPPA technology. The standardized RPPA reagents provided in this kit offer an effective system for generating high-quality data from RPPA assays. In addition, use of this kit can contribute to consistent results between assays, laboratories, and institutions.
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SuperG™ Blocking BufferSEARCH
SuperG™ Blocking Buffer optimizes the use of nitrocellulose for protein assays using fluorescence detection. Results with Super G™ show significant reduction in fluorescence background while maintaining the high protein-binding capacity of porous nitrocellulose. Purified, protein-free solution has high binding efficiency for rapid and comprehensive blocking of non-specific protein.
SuperG™ Plus Protein PreservativeSEARCH
Super G™ Plus Preservation Buffer delivers highly efficient blocking of non-specific protein binding on polymer films, with additional components optimized for long-term protein preservation. This formulation improves assay performance for various proteins stored for extended periods after immobilization on polymer films.