GBL Protein Array Buffer
GBL PROTEIN ARRAY BUFFER
GBL Protein Arraying Buffer is a non-denaturing microarray spotting buffer designed to work with our ONCYTE® porous nitrocellulose film slides.
This buffer formulation is intended for the spotting of antibodies and other proteins where preservation of protein structure and function is imperative for proper antigen recognition during downstream microarray assays.
Applications
- Protein Microarray
- Antigen Capture Assay
- Antibody Capture Assay
References
Anderson, Troy, Julia Wulfkuhle, Emanuel Petricoin, and Raimond L Winslow. "High Resolution Mapping of the Cardiac Transmural Proteome Using Reverse Phase Protein Microarrays." Molecular & Cellular Proteomics : MCP 10, no. 7 (2011): M111.008037. (https://www.ncbi.nlm.nih.gov/pubmed/21490165)
Ecker, Stephanie M., Joshua C. Hines, Scott M. Pfahler, and Bert M. Glaser. "Aqueous Cytokine and Growth Factor Levels Do Not Reliably Reflect Those Levels Found in the Vitreous." Molecular Vision 17 (2011): 2856-863 (https://www.ncbi.nlm.nih.gov/pubmed/22128233)
Kim, Geoffrey, Ben Davidson, Ryan Henning, Junbai Wang, Minshu Yu, Christina Annunziata, Thea Hetland, and Elise C. Kohn. "Adhesion Molecule Protein Signature in Ovarian Cancer Effusions Is Prognostic of Patient Outcome." Cancer 118, no. 6 (2012): 1543-553. (https://www.ncbi.nlm.nih.gov/pubmed/22009736)
QBlock™ Protein Microarray Blocking Buffer is a non-protein based reagent designed to block non-specific protein binding on porous nitrocellulose substrates.This blocker was formulated for maximizing assay signal while minimizing background from assays utilizing Quantum Nanoparticles (QNPs) for detection, and is also compatible with other assay types using organic fluors (e.g. Alexa Fluor dyes, Li-Cor dyes) or colorimetric endpoint detection (e.g DAB) after enzymatic amplification. It is supplied as a 1X solution, ready to use out of the bottle, for microarray applications.
SuperG™ Blocking Buffer optimizes the use of nitrocellulose for protein assays using fluorescence detection. Results with Super G™ show significant reduction in fluorescence background while maintaining the high protein-binding capacity of porous nitrocellulose. Purified, protein-free solution has high binding efficiency for rapid and comprehensive blocking of non-specific protein.
Super G™ Plus Preservation Buffer delivers highly efficient blocking of non-specific protein binding on polymer films, with additional components optimized for long-term protein preservation. This formulation improves assay performance for various proteins stored for extended periods after immobilization on polymer films.
