Protein Crystallization

Protein crystallization is a key assay for structural studies of proteins. The protocols for crystallization of protein are challenging due to the stringent requirement for pure samples and control of environmental conditions during the crystallization process. Vapor diffusion using hanging drop is a preferred method for obtaining quality crystals with…

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ARRAYCAM® MULTIPLEX MICROARRAY IMAGING SYSTEM

Affordable bench-top microarray imager and reagent platform for protein and nucleic acid detection of microarrays printed onto ONCYTE® nitrocellulose coated chips.

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Protein Microarray Substrates

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Grace Bio-Labs microarray surface chemistry is based on the well-known protein-binding properties of nitrocellulose. A range of different formulations have…

APPLICATIONS:

Antigen-Capture AssayAntibody Capture AssayRPPA- Reverse Phase Protein MicroarrayLaser micro-dissection RRPAEpitope-mappingBiomarker Discovery and ValidationImmunogen Discovery

DNA Microarray Substrates

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Epoxy Microarray Slides provide a uniform substrate for a variety of DNA/RNA-based diagnostic applications.

APPLICATIONS:

DNA/Oligonucleotide Microarray ;  microRNA Microarray ;  Single Nucleotide Polymorphism (SNP) Analysis ;  Gene Expression Profiling; 

Microarray Reagents

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Grace Bio-Labs microarray regents have been specifically formulated to achieve the full potential of porous nitrocellulose, accelerating experimental design and…

APPLICATIONS:

Antigen-Capture Assay Antibody Capture Assay RPPA- Reverse Phase Protein Microarray Laser micro-dissection RRPA Epitope-mapping Biomarker Discovery and Validation Immunogen Discovery

APPLICATIONS:

Antibody capture assay ;  Epitope-mapping ;  Biomarker Discovery and Validation ;  Immunogen Discovery ;  Quantitative multiplex immunoassays ;  Peptide Microarrays ;  Autoantibody profiling ;  Multiplex serological assays; 

ProPlate®

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ProPlates® were specifically designed to enable automated robotic liquid handling. Two main configurations are available: The ProPlate® Microtiter Plate is comprised…

APPLICATIONS:

ProteomicsProtein MicroarraysProtein expression analysisAntibody profiling cDNA and oligonucleotide arrays

ARRAYCAM® MULTIPLEX MICROARRAY IMAGING SYSTEM

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Affordable bench-top microarray imager and reagent platform for protein and nucleic acid detection of microarrays printed onto ONCYTE® nitrocellulose coated chips.

APPLICATIONS:

Whole Proteome Screening;  Vaccine Development;  RPPA: Reverse Phase Protein Array;  Biomarker Discovery and Validation;  Epitope Mapping; 

NanoParticle Fluorescent Calibration Slide

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Photostable nanoparticles arrayed on glass slides for calibration of fluorescence imaging systems and quantitative analysis.

APPLICATIONS:

Calibration of Microarray Scanners ;  Quantitative Microarray Analyses Microscope Focal Plane Adjustment;  Microscope Focal Plane Adjustment; 

CoverWell Perfusion Chambers

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CoverWell ™ perfusion press-to-seal covers form water-tight, multiwell cell incubation or cytochemistry chambers when pressed to coverslips or microscope slides.…

APPLICATIONS:

Live-cell imagingMicroscopyImagingSingle molecule spectroscopy

SecureSeal™ Hybridization Chambers

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SecureSeal™ Hybridization Chambers are thin, silicone-gasketed chambers providing optimal surface-to-volume fluid dynamics for hybridization assays on large or multiple specimens…

APPLICATIONS:

In situ hybridizationProtein and DNA MicroarraysImmunocytochemistryRapid microfluidic prototypingFluorescence Resonance Energy Transfer (FRET)

CoverWell™ Incubation Chambers

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CoverWell™ incubation chambers are reusable, easy to apply chambers that attach without the use of adhesive.  CoverWells™ enclose a large…

APPLICATIONS:

Reverse Transfection Microarray;  DNA Microarray;  In-situ hybridization;  Immunohistochemistry; 

Silicone Isolators

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Silicone Isolators allow researchers to isolate specimens using removable hydrophobic barriers. They may be used to isolate cells grown in…

SecureSeal™ Hybridization Chambers

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SecureSeal™ Hybridization Chambers are thin, silicone-gasketed chambers providing optimal surface-to-volume fluid dynamics for hybridization assays on large or multiple specimens…

HybriWell™ Sealing System

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HybriWell™ Sealing System bonds securely to a microscope slide surface in seconds to confine small reagent volumes with samples and…

Hybridization and Incubation

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Hybridization and incubation Seals ad Chambers from Grace Bio-Labs are ideally suited for in situ-hybridization assays. The adhesive seal of…

APPLICATIONS:

In-situ hybridization MicroarraysFluorescence In situ Hybridization (FISH)FRET (Fluorescence Resonance Energy Transfer)

FastWells™ Reagent Barriers

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FastWells™ are sticky, flexible silicone gaskets that form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Gaskets may…

FlexWell™ Incubation Chambers

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FlexWell™ incubation chamber silicone gaskets form wells on slides using clean release adhesive to isolate up to 16 specimens per…

APPLICATIONS:

Protein MicroarrayHybridizationIncubation

HybriSlip™ Hybridization Covers

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HybriSlips™ are rigid, light-weight, thin plastic coverslips that minimize friction and facilitate uniform reagent distribution during incubation steps which require…

ProPlates®

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ProPlates® were specifically designed to enable automated robotic liquid handling. Two main configurations are available: The ProPlate® Microtiter Plate is comprised…

APPLICATIONS:

ProteomicsProtein MicroarraysProtein expression analysis;  Antibody profiling ;  cDNA and oligonucleotide arrays; 

Silicone Isolators™ Sheet Material

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Silicone isolator™ sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. Where additional sealing is…

APPLICATIONS:

Protein and DNA arrays ;  Immunohistochemistry;  Fluorescence In situ Hybridization (FISH) ;  Biopolymers and hydrogel formulation ;  Cryogenic-transmission electron microscopy (Cryo-TEM) ;  Microwave crystallization ;  Ultra-small-angle X-ray scattering (USAXS) ;  Tissue ingeneering;  Live cell lithography” (LCL); 

Imaging Spacers

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Imaging spacers are ultra-thin adhesive spacers which peel-and-stick to coverglass or microscope slides to confine specimens without compression. Layer multiple…

APPLICATIONS:

Imaging;  Microscopy;  High-temperature single-molecule kinetic analysis;  Anti‐Stokes Raman scattering microscopy; 

CoverWell™ Imaging Chambers

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CoverWell ™ imaging chambers are designed to stabilize and support thick and free-floating specimens for confocal microscopy and imaging applications.…

APPLICATIONS:

Confocal microscopy Imaging Tissue and Cell staining ;  High Resolution Microscopy ;  Live-cell imaging ; 

CoverWell™ Perfusion Chambers

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CoverWell ™ perfusion press-to-seal covers form water-tight, multiwell cell incubation or cytochemistry chambers when pressed to coverslips or microscope slides.…

APPLICATIONS:

Single molecule spectroscopy Live-cell imaging Microscoscopy

FastWells™ Reagent Barriers

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FastWells™ are sticky, flexible silicone gaskets that form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Gaskets may…

APPLICATIONS:

Microscopy Fluorescence In situ Hybridization (FISH) Single-molecule fluorescence analysis ;  Immunohistochemistry ; 

MultiSlip™ Coverglass Inserts

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MutliSlip™ inserts with 8 (18mm x 18mm) or 15 (12mm x 12mm) No. 1.5 German glass coverglass per insert are…

APPLICATIONS:

High resolution microscopy Fluorescent imaging Immunohistochemistry ;  Cell Culture; 

SecureSeal™ Adhesive Sheets

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These adhesive sheets are made using the same SecureSeal™ adhesive as is used to make HybriWell™ and SecureSeal™ Incubation Chambers.  Thin,…

APPLICATIONS:

Imaging ;  Tissue and Cell staining ;  High Resolution Microscopy; 

SecureSlip™ Silicone Supported Coverglass

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SecureSlip™ Silicone Supported Coverglass is affixed to a thin microscopically transparent silicone base which secures it to culture vessels by…

Imaging and Microscopy

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Imaging seals and chambers from Grace Bio-Labs offer a selection of tools for cell/tissue staining for high quality results in…

APPLICATIONS:

Tissue and Cell stainingHigh Resolution MicroscopyLive-cell imaging

CultureWell™ MultiWell Chambered Coverslips

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CultureWell™ chambered coverglass products consist of removable and reusable, non-cytotoxic silicone gaskets secured to number 1.5 German coverglass. Chambered coverglass…

APPLICATIONS:

Cell Culture Fluorescence applications In-situ hybridization Immunostaining

CS16-CultureWell™ Removable Chambered Coverglass

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CS16 CultureWell™ removable chambered coverglass is a 16-well chambered coverglass cell culture vessel, with 2 x 8 format with standard…

APPLICATIONS:

Cell CultureFluorescence applicationsIn-situ hybridizationImmunostaining

CultureWell™ Coverglass Inserts

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Each CultureWell™ coverglass insert is comprised of four chambered coverglass, assembled in a disposable frame placed in a standard 86mm…

APPLICATIONS:

High resolution microscopy Fluorescent imaging Immunohistochemistry

CultureWell™ Reusable Gaskets

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Gaskets are ideal for forming wells on glass microscope slides or in polystyrene dishes. Gaskets are non-sterile and may be…

APPLICATIONS:

Cell CultureHigh resolution microscopyFluorescent imaging Immunohistochemistry

CultureWell™ Silicone Sheet Material

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CultureWell™ clear silicone sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. They may be…

APPLICATIONS:

Cell CultureHigh resolution microscopy Fluorescent imagingImmunohistochemistry

MultiSlip™ Coverglass Inserts

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MutliSlip™ inserts with 8 (18mm x 18mm) or 15 (12mm x 12mm) No. 1.5 German glass coverglass per insert are…

APPLICATIONS:

Cell CultureFluorescent imaging Immunohistochemistry

SecureSlip™ Silicone Supported Coverglass

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SecureSlip™ Silicone Supported Coverglass is affixed to a thin microscopically transparent silicone base which secures it to culture vessels by…

APPLICATIONS:

Cell CultureImmunofluorescence assayMicroscopy

CultureWell™ ChamberSLIP 16, Non-Removable Chambered Coverglass

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CultureWell™ NON Removable Chambered Coverglass, 16 Well, No. 1.5 German borosilicate Coverglass. Product consists of cell culture vessels, with a…

APPLICATIONS:

Cell Culture Fluorescence applicationsSmall volume incubation Immunostaining

Silicone Wound Splints

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Wound splints are constructed of silicone and include suture sites for increased precision in affixing on or within an animal…

Silicone Isolator Sheet Material

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Silicone isolator™ sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. Where additional sealing is…

APPLICATIONS:

Protein and DNA arrays ;  Immunohistochemistry ;  Fluorescence In-situ Hybridization (FISH) ;  Biopolymers and hydrogel formulation;  Cryogenic-transmission electron microscopy (Cryo-TEM) ;  X-ray scattering ;  Microwave crystallization ;  Ultra-small-angle X-ray scattering (USAXS) ;  Tissue engineering Live cell lithography (LCL); 

CultureWell Silicone Sheet Material

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CultureWell™ clear silicone sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. They may be…

APPLICATIONS:

Lorem Ipsum ;  Lorem Ipsum;  Lorem Ipsum; 

ARRAYCAM® MULTIPLEX MICROARRAY IMAGING SYSTEM

ARRAYCAM® MULTIPLEX MICROARRAY IMAGING SYSTEM was developed by Grace Bio-Labs as an affordable bench-top microarray imager and reagent platform for protein and nucleic acid detection of microarrays printed onto ONCYTE® nitrocellulose coated chips.

The ArrayCAM® 400S Imager combines imaging capability for both colorimetric and multiple fluorescent labels into one instrument.   This CMOS, camera-based imager, allows microarray data to be collected at 10 µm resolution in a fraction of the time required by conventional digitizing scanners.  Typical image capture takes less than one minute per 25 x 75mm chip.

The high power, UV diode laser produces an excitation wavelength ideally suited to excite a full spectrum of Qdot® labeled detection reagents as well as Fast Green and SYPRO Ruby® stains commonly used to quantify total protein.  Interchangeable filters onboard the instrument support a large number of multiplexing applications allowing investigators to measure several biomarkers, at the same time on a single spot within the same microarray without a loss of sensitivity.

User-friendly, software provides a full menu of features to optimize imaging and analysis.  16-bit files may be exported in a variety of formats including TIFF and PNG.  Tools for automated image analysis produce data output from full-slide images, in seconds.    

* Qdot® and SYPRO Ruby® are registered Trademarks of Life Technologies Inc.

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ARRAYCAM® 400S IMAGER

 

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ArrayCAM® 400S is a personal microarray imager designed for the rapid capture and analysis of microarrays printed onto ONCYTE nitrocellulose coated chips. This portable imager is ideally suited for the simultaneous detection of multiple biomarkers using several Qdot® labeled detection probes*, certain fluorescent dyes and precipitating colorimetric assay results. Software included with the instrument features a selectable menu of parameters to optimize image capture and analysis. Automatic mapping, preview and flagging of spots which do not conform to user selected criteria saves time and provides consistency in data output. For a complete description of features and how to use them please refer to the ArrayCAM® 400S Operation Manual * Qdot® is a registered Trademark of Life Technologies Inc.

Videos

Tutorial 1: Capture

Tutorial 2: Image Adjustments 

Tutorial 3: GAL Files

Tutorial 4: Analysis Options

References

Nakajima R, Supnet M, Jasinskas A, Jain A, Taghavian O, Obiero J, Milton DK, Chen WH, Grantham M, Webby R, Krammer F, Carter D, Felgner PL, Davies DH. "Protein Microarray Analysis of the Specificity and Cross-Reactivity of Influenza Virus Hemagglutinin-Specific Antibodies." mSphere. 2018: https://msphere.asm.org/content/3/6/e00592-18 

Taghavian, O., Jain, A., Joyner, C. J., Ketchum, S., Nakajima, R., Jasinskas, A., Liang, L., Fong, R., King, C., Greenhouse, B., Murphy, M., Bailey, J., Galinski, M. R., Barnwell, J. W., P., Christopher V., Davies, D. H., Felgner, P. L. "Antibody Profiling by Proteome Microarray with Multiplex Isotype Detection Reveals Overlap between Human and Aotus nancymaae Controlled Malaria Infections." PROTEOMICS, January 1, 2018. http://onlinelibrary.wiley.com/doi/10.1002/pmic.201700277/abstract

Felgner J, Jain A, Nakajima R, Liang L, Jasinskas A, Gotuzzo E, Vinetz JM, Miyajima F, Pirmohamed M, Hassan-Hanga F, Umoru D, Jibir BW, Gambo S, Olateju K, Felgner PL, Obaro S, Davies DH. "Development of ELISAs for diagnosis of acute typhoid fever in Nigerian children." PLoS Negl Trop Dis. 2017: https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0005679.

Charles RC, Nakajima R, Liang L, Jasinskas A, Berger A, Leung DT, Kelly M, Xu P, Kovác P, Giffen SR, Harbison JD, Chowdhury F, Khan AI, Calderwood SB, Bhuiyan TR, Harris JB, Felgner PL, Qadri F, Ryan ET."Plasma and Mucosal Immunoglobulin M, Immunoglobulin A, and Immunoglobulin G Responses to the Vibrio cholerae O1 Protein Immunome in Adults With Cholera in Bangladesh." J Infect Dis. 2017. https://academic.oup.com/jid/article/216/1/125/3848946

Uplekar S, Rao PN, Ramanathapuram L, Awasthi V, Verma K, Sutton P, Ali SZ, Patel A, G SL, Ravishankaran S, Desai N, Tandel N, Choubey S, Barla P, Kanagaraj D, Eapen A, Pradhan K, Singh R, Jain A, Felgner PL, Davies DH, Carlton JM, Das J. Characterizing Antibody Responses to Plasmodium vivax and Plasmodium falciparum Antigens in India Using Genome-Scale Protein Microarrays. PLoS Negl Trop Dis. 2017: https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0005323

Jain A, Taghavian O, Vallejo D, Dotsey E, Schwartz D, Bell FG, Greef C, Davies DH, Grudzien J, Lee AP, Felgner PL, Liang L. Evaluation of quantum dot immunofluorescence and a digital CMOS imaging system as an alternative to conventional organic fluorescence dyes and laser scanning for quantifying protein microarrays. Proteomics. 2016: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3318641/

Kalantari-Dehaghi, Mina, Sookhee Chun, Aziz Alami Chentoufi, Jozelyn Pablo, Li Liang, Gargi Dasgupta, Douglas M. Molina, et al. “Discovery of Potential Diagnostic and Vaccine Antigens in Herpes Simplex Virus 1 and 2 by Proteome-Wide Antibody Profiling.” Journal of Virology 86, no. 8 (April 2012): 4328–39. http://doi.org/10.1128/JVI.05194-11.

Cruz-Fisher, Maria I., Chunmei Cheng, Guifeng Sun, Sukumar Pal, Andy Teng, Douglas M. Molina, Matthew A. Kayala, et al. “Identification of Immunodominant Antigens by Probing a Whole Chlamydia Trachomatis Open Reading Frame Proteome Microarray Using Sera from Immunized Mice.” Infection and Immunity 79, no. 1 (1, 2011): 246–57. http://doi.org/10.1128/IAI.00626-10.
 
Driguez, Patrick, Yuesheng Li, Soraya Gaze, Mark S. Pearson, Rie Nakajima, Angela Trieu, Denise L. Doolan, et al. “Antibody Signatures Reflect Different Disease Pathologies in Patients With Schistosomiasis Due to Schistosoma Japonicum.” The Journal of Infectious Diseases 213, no. 1 (January 1, 2016): 122–30. http://doi.org/10.1093/infdis/jiv356.
 
F. G., Bell, Greef C., Barton D. K., Wells J., Grudzien J., and McGrath C. M. “An Integrated Digital Imaging System and Microarray Mapping Software for Rapid Multiplexed Quantitation of Protein Microarray Immunoassays,” n.d. https://gracebio.com/wp-content/uploads/2017/07/An-Integrated-Digital-Imaging-System-.pdf.
 
Teng, Andy, Maria I. Cruz-Fisher, Chunmei Cheng, Sukumar Pal, Guifeng Sun, Pooja Ralli-Jain, Douglas M. Molina, Philip L. Felgner, Xiaowu Liang, and Luis M. de la Maza. “Proteomic Identification of Immunodominant Chlamydial Antigens in a Mouse Model.” Journal of Proteomics 77 (December 21, 2012): 176–86. http://doi.org/10.1016/j.jprot.2012.08.017.
 
Patton, Dorothy L., Andy Teng, Arlo Randall, Xiaowu Liang, Philip L. Felgner, and Luis M. de la Maza. “Whole Genome Identification of C. Trachomatis Immunodominant Antigens after Genital Tract Infections and Effect of Antibiotic Treatment of Pigtailed Macaques.” Journal of Proteomics 108 (August 28, 2014): 99–109. http://doi.org/10.1016/j.jprot.2014.05.009.

 

FAQ

Q: What methods does the ArrayCAM software use to locate spots?

A: There are two different methods.

1. Fiducial template method

This method requires fiducials to be printed and developed. The fiducials will be located at the top of the microarray as the first row in the array. A template is created by drawing a rectangle around the fiducial row. Once the template is created, it can be saved and used for all microarrays that have the same configuration (print map). Batch Analysis uses this method.

2. Non-fiducial method

In this method, you need to place a location rectangle around the first row in the microarray (via the software). This will need to be repeated for each image to be analyzed.

Q: Do you need a gal file to perform spot analysis?

A: Yes. And you need to tag each spot in the gal file according to its type in column 6.

Q: What are the permitted spot tags?

A: The tags are Fiducial, Positive, Negative, Analyte, Buffer, Blank, and Control. The tags do not need to be capitalized, but they do need to be spelled correctly. The software will locate and analyze spots corresponding to any combination of the seven tags.

Q: What is the format of the results file?

A: Tab-delimited text with column headers.

Q: How big is the ArrayCAM?

A: Overall dimensions: 8.23in x 10.6in x 14.02in (WxDXH). Weight: 19.6 lb.

Q: How long does Batch Analysis take?

A: The software analyzes approximately 100 spots per second, so it depends on how many arrays are being analyzed and the number of spots in each array. An 8-pad slide with 1156 spots (17x68 array) on each pad takes about 1.5 minutes to analyze.

Q: How should the slide be oriented in the ArrayCAM for imaging?

A: When inserting a slide into the imager, the content needs to be facing down and the top of the slide needs to be to the right.

ARRAYCAM® MULTIPLEX MICROARRAY IMAGING SYSTEM

Contact us

Affordable bench-top microarray imager and reagent platform for protein and nucleic acid detection of microarrays printed onto ONCYTE® nitrocellulose coated chips.

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