FastWells™ Reagent Barriers

FASTWELLS™ REAGENT BARRIERS

FastWells™ reagent barriers are sticky, flexible silicone gaskets that form hydrophobic barriers around specimens without messy adhesives or special slides. Gaskets may be stacked to increase depth and volume.

FastWells™ provide rapid isolation of cells and tissues on slides or coverslips for antibody incubations. The barriers remain sealed during agitated washing steps, preventing reagents from spilling when shaken or rocked. FastWells™ peel off quickly and cleanly, leaving no residue to interfere with specimen cover-slipping. They can be washed, autoclaved and reused. The barriers can also be sealed to form incubation chambers using flexible HybriSlip™ covers.

Technical Resources

Instructions

Instructions for Use - Silicone Isolators

Applications

  • Microscopy

  • Fluorescence In situ Hybridization (FISH)

  • Single-molecule fluorescence analysis

  • Immunohistochemistry

Videos

Silicone Isolator Application

 

References

Yokomizo, T., & Dzierzak, E. (2010). Three-dimensional cartography of hematopoietic clusters in the vasculature of whole mouse embryos. Development, 137(21), 3651–3661. 

Yokomizo, T., Yamada-Inagawa, T., Yzaguirre, A. D., Chen, M. J., Speck, N. A., & Dzierzak, E. (2012). Whole-mount three-dimensional imaging of internally localized immunostained cells within mouse embryos.Nature Protocols, 7(3), 421–431.

Wang, Y.-H., Collins, A., Guo, L., Smith-Dupont, K. B., Gai, F., Svitkina, T., & Janmey, P. A. (2012). Divalent Cation-Induced Cluster Formation by Polyphosphoinositides in Model Membranes. Journal of the American Chemical Society, 134(7), 3387–3395. 

To see more references related to this product click here.