Silicone Isolators™
SILICONE ISOLATORS
Silicone Isolators allow researchers to isolate specimens using removable hydrophobic barriers. They may be used to isolate cells grown in culture dishes or separate multiple specimens affixed to microscope slides. Isolators are available in red silicone thickness 0.5 to 2.5mm pre-cut or available as sheet material with or without secure seal clean adhesive on one or both surfaces. Nonadhesive isolators are autoclavable and reusable.
Silicone Isolators remain sealed to smooth surfaces during washing steps. Where additional sealing is required, SecureSeal™ adhesive on one or both surfaces is recommended. Each seal design is also available in CultureWell™ “water-clear” silicone, which is highly recommended for sensitive cell culture applications. Closed chambers may be formed using flexible RNase free, HybriSlip ™ covers. Recommended use temperature is -62°C to 218°C.
Silicone Isolators are also available in sheets, visit Silicone isolator™ sheet material. If you are looking for a thinner solution see our Imaging spacers and the SecureSeal™ adhesive sheets.
Applications
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Protein and DNA Microarrays
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Hydrogel
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Immunohistochemistry
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Fluorescence In situ Hybridization (FISH)
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Biopolymers
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Imaging
References
Gong, P., Harbers, G. M., & Grainger, D. W. (2006). Multi-technique comparison of immobilized and hybridized oligonucleotide surface density on commercial amine-reactive microarray slides. Analytical Chemistry, 78(7), 2342–2351.
Li, M. W., Biggers, J. D., Elmoazzen, H. Y., Toner, M., McGinnis, L., & Lloyd, K. C. K. (2007). Long-term storage of mouse spermatozoa after evaporative drying. Reproduction (Cambridge, England), 133(5), 919–929.
Ho, D., Falter, K., Severin, P., & Gaub, H. E. (2009). DNA as a Force Sensor in an Aptamer-Based Biochip for Adenosine. Analytical Chemistry, 81(8), 3159–3164.
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Videos
Silicone Isolator Application
FAQ
Q: Do the non-adhesive reusable isolators adhere to the glass slide and allow a small volume of liquid to be contained? Are the isolators with adhesive more effective?
A: Typically for most applications, the silicone isolator (without PSA) adheres very well and can be removed easily.
Silicone Isolators allow researchers to isolate specimens using removable hydrophobic barriers. They may be used to isolate cells grown in culture dishes or separate multiple specimens affixed to microscope slides. Isolators are available in red silicone thickness 0.5 to 2.5mm pre-cut or as sheet material with or without secure seal clean adhesive on one or both surfaces. Nonadhesive isolators are autoclavable and reusable.Silicone Isolators™ remain sealed to smooth surfaces during washing steps. Where additional sealing is required, SecureSeal™ adhesive on one or both surfaces is recommended.
