SecureSeal™ Hybridization Chambers

SECURESEAL™ HYBRIDIZATION CHAMBERS

SecureSeal™ Hybridization Chambers are thin, silicone-gasketed chambers providing optimal surface-to-volume fluid dynamics for hybridization assays on large or multiple specimens and microarrays on glass or film coated slides. These adhesive-backed chambers are designed for single-use, very-low volume incubations and are ideal for autoradiographic, fluorescent or chemiluminescent end-point applications requiring small, enclosed fluid volumes. SecureSeal™ Adhesive is a double-sided tape with a silicone adhesive surface on one side (covered with a clear PET liner) and an acrylic adhesive on the other surface (covered with a paper liner).

SecureSeal™ chambers form removable, peel-and-stick enclosures to isolate specimens affixed to glass microscope slides. The chamber design minimizes friction, promotes reagent mixing, and facilitates uniform hybridization. Sealable access ports in the chamber surface allow for the addition and removal of reactants. SecureSeal™ adhesive bonds chambers to glass in seconds and removes cleanly and easily even after heating. Adhesive Seal Tabs create leak-proof chambers that are temperature resistant. Each package includes illustrated instructions for use and 120 adhesive port seals. Additional seal tabs are sold separately (SKU: 629200).

Note: If you are using Cy5® of Alexa Fluor® 647 direct-labeled DNA probes, please refer to our “fluorescent friendly” chambers, or contact us for customizing details

Technical Resources

Instructions

Instructions for Use - SecureSeal

Applications

  • In situ hybridization
  • Protein and DNA Microarrays
  • Immunocytochemistry
  • DNA Amplification
  • Neurophysiology
  • Reaction kinetics
  • Rapid microfluidic prototyping
  • Nucleic Acid Programmable Protein Arrays
  • Fluorescence Resonance Energy Transfer (FRET)
  • Single molecule fluorescence microscopy

References

Zhang, H., Kay, A., Forsyth, N. R., Liu, K.-K., & El Haj, A. J. (2012). Gene expression of single human mesenchymal stem cell in response to fluid shear. Journal of Tissue Engineering, 3(1). 

Bar, K. J., Tsao, C., Iyer, S. S., Decker, J. M., Yang, Y., Bonsignori, M., … Shaw, G. M. (2012). Early Low-Titer Neutralizing Antibodies Impede HIV-1 Replication and Select for Virus Escape. PLoS Pathog, 8(5), e1002721.

Roberts, M. R., Hendrickson, A., McGuire, C. R., & Reh, T. A. (2005). Retinoid X receptor (gamma) is necessary to establish the S-opsin gradient in cone photoreceptors of the developing mouse retina.Investigative Ophthalmology & Visual Science, 46(8), 2897–2904. 

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