Protein Crystallization

Protein crystallization is a key assay for structural studies of proteins. The protocols for crystallization of protein are challenging due to the stringent requirement for pure samples and control of environmental conditions during the crystallization process. Vapor diffusion using hanging drop is a preferred method for obtaining quality crystals with…

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ARRAYCAM® MULTIPLEX MICROARRAY IMAGING SYSTEM

Affordable bench-top microarray imager and reagent platform for protein and nucleic acid detection of microarrays printed onto ONCYTE® nitrocellulose coated chips.

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Protein Microarray Substrates

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Grace Bio-Labs microarray surface chemistry is based on the well-known protein-binding properties of nitrocellulose. A range of different formulations have…

APPLICATIONS:

Antigen-Capture AssayAntibody Capture AssayRPPA- Reverse Phase Protein MicroarrayLaser micro-dissection RRPAEpitope-mappingBiomarker Discovery and ValidationImmunogen Discovery

DNA Microarray Substrates

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Epoxy Microarray Slides provide a uniform substrate for a variety of DNA/RNA-based diagnostic applications.

APPLICATIONS:

DNA/Oligonucleotide Microarray ;  microRNA Microarray ;  Single Nucleotide Polymorphism (SNP) Analysis ;  Gene Expression Profiling; 

Microarray Reagents

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Grace Bio-Labs microarray regents have been specifically formulated to achieve the full potential of porous nitrocellulose, accelerating experimental design and…

APPLICATIONS:

Antigen-Capture Assay Antibody Capture Assay RPPA- Reverse Phase Protein Microarray Laser micro-dissection RRPA Epitope-mapping Biomarker Discovery and Validation Immunogen Discovery

APPLICATIONS:

Antibody capture assay ;  Epitope-mapping ;  Biomarker Discovery and Validation ;  Immunogen Discovery ;  Quantitative multiplex immunoassays ;  Peptide Microarrays ;  Autoantibody profiling ;  Multiplex serological assays; 

ProPlate®

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ProPlates® were specifically designed to enable automated robotic liquid handling. Two main configurations are available: The ProPlate® Microtiter Plate is comprised…

APPLICATIONS:

ProteomicsProtein MicroarraysProtein expression analysisAntibody profiling cDNA and oligonucleotide arrays

ARRAYCAM® MULTIPLEX MICROARRAY IMAGING SYSTEM

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Affordable bench-top microarray imager and reagent platform for protein and nucleic acid detection of microarrays printed onto ONCYTE® nitrocellulose coated chips.

APPLICATIONS:

Whole Proteome Screening;  Vaccine Development;  RPPA: Reverse Phase Protein Array;  Biomarker Discovery and Validation;  Epitope Mapping; 

NanoParticle Fluorescent Calibration Slide

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Photostable nanoparticles arrayed on glass slides for calibration of fluorescence imaging systems and quantitative analysis.

APPLICATIONS:

Calibration of Microarray Scanners ;  Quantitative Microarray Analyses Microscope Focal Plane Adjustment;  Microscope Focal Plane Adjustment; 

CoverWell Perfusion Chambers

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CoverWell ™ perfusion press-to-seal covers form water-tight, multiwell cell incubation or cytochemistry chambers when pressed to coverslips or microscope slides.…

APPLICATIONS:

Live-cell imagingMicroscopyImagingSingle molecule spectroscopy

SecureSeal™ Hybridization Chambers

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SecureSeal™ Hybridization Chambers are thin, silicone-gasketed chambers providing optimal surface-to-volume fluid dynamics for hybridization assays on large or multiple specimens…

APPLICATIONS:

In situ hybridizationProtein and DNA MicroarraysImmunocytochemistryRapid microfluidic prototypingFluorescence Resonance Energy Transfer (FRET)

CoverWell™ Incubation Chambers

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CoverWell™ incubation chambers are reusable, easy to apply chambers that attach without the use of adhesive.  CoverWells™ enclose a large…

APPLICATIONS:

Reverse Transfection Microarray;  DNA Microarray;  In-situ hybridization;  Immunohistochemistry; 

Silicone Isolators

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Silicone Isolators allow researchers to isolate specimens using removable hydrophobic barriers. They may be used to isolate cells grown in…

SecureSeal™ Hybridization Chambers

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SecureSeal™ Hybridization Chambers are thin, silicone-gasketed chambers providing optimal surface-to-volume fluid dynamics for hybridization assays on large or multiple specimens…

HybriWell™ Sealing System

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HybriWell™ Sealing System bonds securely to a microscope slide surface in seconds to confine small reagent volumes with samples and…

Hybridization and Incubation

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Hybridization and incubation Seals ad Chambers from Grace Bio-Labs are ideally suited for in situ-hybridization assays. The adhesive seal of…

APPLICATIONS:

In-situ hybridization MicroarraysFluorescence In situ Hybridization (FISH)FRET (Fluorescence Resonance Energy Transfer)

FastWells™ Reagent Barriers

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FastWells™ are sticky, flexible silicone gaskets that form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Gaskets may…

FlexWell™ Incubation Chambers

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FlexWell™ incubation chamber silicone gaskets form wells on slides using clean release adhesive to isolate up to 16 specimens per…

APPLICATIONS:

Protein MicroarrayHybridizationIncubation

HybriSlip™ Hybridization Covers

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HybriSlips™ are rigid, light-weight, thin plastic coverslips that minimize friction and facilitate uniform reagent distribution during incubation steps which require…

ProPlates®

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ProPlates® were specifically designed to enable automated robotic liquid handling. Two main configurations are available: The ProPlate® Microtiter Plate is comprised…

APPLICATIONS:

ProteomicsProtein MicroarraysProtein expression analysis;  Antibody profiling ;  cDNA and oligonucleotide arrays; 

Silicone Isolators™ Sheet Material

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Silicone isolator™ sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. Where additional sealing is…

APPLICATIONS:

Protein and DNA arrays ;  Immunohistochemistry;  Fluorescence In situ Hybridization (FISH) ;  Biopolymers and hydrogel formulation ;  Cryogenic-transmission electron microscopy (Cryo-TEM) ;  Microwave crystallization ;  Ultra-small-angle X-ray scattering (USAXS) ;  Tissue ingeneering;  Live cell lithography” (LCL); 

Imaging Spacers

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Imaging spacers are ultra-thin adhesive spacers which peel-and-stick to coverglass or microscope slides to confine specimens without compression. Layer multiple…

APPLICATIONS:

Imaging;  Microscopy;  High-temperature single-molecule kinetic analysis;  Anti‐Stokes Raman scattering microscopy; 

CoverWell™ Imaging Chambers

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CoverWell ™ imaging chambers are designed to stabilize and support thick and free-floating specimens for confocal microscopy and imaging applications.…

APPLICATIONS:

Confocal microscopy Imaging Tissue and Cell staining ;  High Resolution Microscopy ;  Live-cell imaging ; 

CoverWell™ Perfusion Chambers

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CoverWell ™ perfusion press-to-seal covers form water-tight, multiwell cell incubation or cytochemistry chambers when pressed to coverslips or microscope slides.…

APPLICATIONS:

Single molecule spectroscopy Live-cell imaging Microscoscopy

FastWells™ Reagent Barriers

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FastWells™ are sticky, flexible silicone gaskets that form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Gaskets may…

APPLICATIONS:

Microscopy Fluorescence In situ Hybridization (FISH) Single-molecule fluorescence analysis ;  Immunohistochemistry ; 

MultiSlip™ Coverglass Inserts

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MutliSlip™ inserts with 8 (18mm x 18mm) or 15 (12mm x 12mm) No. 1.5 German glass coverglass per insert are…

APPLICATIONS:

High resolution microscopy Fluorescent imaging Immunohistochemistry ;  Cell Culture; 

SecureSeal™ Adhesive Sheets

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These adhesive sheets are made using the same SecureSeal™ adhesive as is used to make HybriWell™ and SecureSeal™ Incubation Chambers.  Thin,…

APPLICATIONS:

Imaging ;  Tissue and Cell staining ;  High Resolution Microscopy; 

SecureSlip™ Silicone Supported Coverglass

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SecureSlip™ Silicone Supported Coverglass is affixed to a thin microscopically transparent silicone base which secures it to culture vessels by…

Imaging and Microscopy

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Imaging seals and chambers from Grace Bio-Labs offer a selection of tools for cell/tissue staining for high quality results in…

APPLICATIONS:

Tissue and Cell stainingHigh Resolution MicroscopyLive-cell imaging

CultureWell™ MultiWell Chambered Coverslips

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CultureWell™ chambered coverglass products consist of removable and reusable, non-cytotoxic silicone gaskets secured to number 1.5 German coverglass. Chambered coverglass…

APPLICATIONS:

Cell Culture Fluorescence applications In-situ hybridization Immunostaining

CS16-CultureWell™ Removable Chambered Coverglass

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CS16 CultureWell™ removable chambered coverglass is a 16-well chambered coverglass cell culture vessel, with 2 x 8 format with standard…

APPLICATIONS:

Cell CultureFluorescence applicationsIn-situ hybridizationImmunostaining

CultureWell™ Coverglass Inserts

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Each CultureWell™ coverglass insert is comprised of four chambered coverglass, assembled in a disposable frame placed in a standard 86mm…

APPLICATIONS:

High resolution microscopy Fluorescent imaging Immunohistochemistry

CultureWell™ Reusable Gaskets

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Gaskets are ideal for forming wells on glass microscope slides or in polystyrene dishes. Gaskets are non-sterile and may be…

APPLICATIONS:

Cell CultureHigh resolution microscopyFluorescent imaging Immunohistochemistry

CultureWell™ Silicone Sheet Material

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CultureWell™ clear silicone sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. They may be…

APPLICATIONS:

Cell CultureHigh resolution microscopy Fluorescent imagingImmunohistochemistry

MultiSlip™ Coverglass Inserts

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MutliSlip™ inserts with 8 (18mm x 18mm) or 15 (12mm x 12mm) No. 1.5 German glass coverglass per insert are…

APPLICATIONS:

Cell CultureFluorescent imaging Immunohistochemistry

SecureSlip™ Silicone Supported Coverglass

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SecureSlip™ Silicone Supported Coverglass is affixed to a thin microscopically transparent silicone base which secures it to culture vessels by…

APPLICATIONS:

Cell CultureImmunofluorescence assayMicroscopy

CultureWell™ ChamberSLIP 16, Non-Removable Chambered Coverglass

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CultureWell™ NON Removable Chambered Coverglass, 16 Well, No. 1.5 German borosilicate Coverglass. Product consists of cell culture vessels, with a…

APPLICATIONS:

Cell Culture Fluorescence applicationsSmall volume incubation Immunostaining

Silicone Wound Splints

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Wound splints are constructed of silicone and include suture sites for increased precision in affixing on or within an animal…

Silicone Isolator Sheet Material

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Silicone isolator™ sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. Where additional sealing is…

APPLICATIONS:

Protein and DNA arrays ;  Immunohistochemistry ;  Fluorescence In-situ Hybridization (FISH) ;  Biopolymers and hydrogel formulation;  Cryogenic-transmission electron microscopy (Cryo-TEM) ;  X-ray scattering ;  Microwave crystallization ;  Ultra-small-angle X-ray scattering (USAXS) ;  Tissue engineering Live cell lithography (LCL); 

CultureWell Silicone Sheet Material

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CultureWell™ clear silicone sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. They may be…

APPLICATIONS:

Lorem Ipsum ;  Lorem Ipsum;  Lorem Ipsum; 

HybriWell™ Sealing System

HYBRIWELL ™ SEALING SYSTEM

HybriWell ™ Sealing System bonds securely and rapidly to a microscope slide surface to confine small reagent volumes with samples. HybriWells™ are manufactured using a clear plastic polycarbonate cover (0.010”- 0.254mm thick) and double-sided adhesives (chamber). Two access ports (1.5mm diameter) are added to each chamber to allow for the addition or removal of reactants. Ports are easily sealed using Adhesive Seal Tabs (200 tabs are included), see the video. Sealed chambers are watertight and ideally suited for submerged or overnight incubations. RNase free, hydrophobic surfaces will not trap or bind probes. Press fit tubing connectors provide affordable and easy means to connect HybriWells™ to pumps and other devices for fluidic exchange.

If you are using Cy5 or ® Alexa Fluor 647 direct-labeled DNA probes please refer to our new “fluorescent friendly” chambers, see Fluorescent Friendly adhesive tab for more information. All current designs may be fabricated using our “fluorescent friendly” adhesive material.

Chamber depth and dimensions could easily be customized, a variety of adhesive options are also available for applications that employ the use of “exotic” surface chemistries.

Grace Bio-Labs, has a large portfolio of adhesive options, plastics, and know-how to tailor a solution to fit your needs.

 

Fluorescent Friendly Adhesive

Standard versions of the HybriWell™ are manufactured using double-sided, clear SecureSeal™ Adhesive. The Fluorescent Friendly Adhesive (black) was developed in response to reports from few laboratories that they were experiencing signal quenching using CY5 and AF647 using SecureSeal™ Adhesive. We contacted multiple HybriWell™ customers using these fluors for hybridization and results were mixed with many of the labs having no issues whatsoever. After creating a matrix of variables, the information we know to date is;

  • The phenomenon only occurs during hybridization assays above 55C
  • Only occurs if the fluor is directly conjugated to the probe (no problem in a sandwich assay).
  • CY3 and other fluors are not affected.
  • Some labs do not have the quenching issue at all under any of the above conditions.

As far as we know, some component of the hybridization buffer is reacting with some component of the adhesive used to make standard HybriWell™ and SecureSeal™ products resulting in signal quenching. We are not able to identify which component may be the culprit as many of the customers having the problem were using pre-made hybe buffers, the manufacturers of which will not share the formulation with us.  We feel that it is appropriate to alert customers to the potential risk of this quenching phenomenon if they are using SecureSeal™ adhesive under any of the conditions we’ve described. We identified the Fluorescent Friendly adhesive as a material which does not quench signal under any conditions and it was tested by the labs which reported the problem and all reported favorable results.

Technical Resources

Instructions

Instructions for Use HybriWell

Applications

  • In situ hybridization
  • Protein and DNA Microarrays
  • Immunocytochemistry
  • DNA Amplification
  • Neurophysiology
  • Reaction kinetics
  • Rapid microfluidic prototyping
  • Nucleic Acid Programmable Protein Arrays
  • Fluorescence Resonance Energy Transfer (FRET)
  • Single molecule fluorescence microscopy

References

Vang S, Seitz K, Krysan PJ. "A simple microfluidic device for live cell imaging of Arabidopsis cotyledons, leaves, and seedlings." Biotechniques. 2018 Jun;64(6):255-261. 

 2018 Jun;64(6):255-261. doi: 10.2144/btn-2018-0044.

Siebman, Coralie, Orlin D Velev, and Vera I Slaveykova. "Two-Dimensional Algal Collection and Assembly by Combining AC-Dielectrophoresis with Fluorescence Detection for Contaminant-Induced Oxidative Stress Sensing." Biosensors 5, no. 2 (2015): 319-36.

Jeenduang, Nutjaree, Sureerut Porntadavity, Markus Von Nickisch-Rosenegk, Frank F Bier, and Chamras Promptmas. "Two-dye Based Arrayed Primer Extension for Simultaneous Multigene Detection in Lipid Metabolism.Clinica Chimica Acta; International Journal of Clinical Chemistry 442 (2015): 36-43.
 

Helmer, Dorothea, Ina Rink, James A. R. Dalton, Kevin Brahm, Marina Jöst, Tobias M. Nargang, Witali Blum, et al. “Rational Design of a Peptide Capture Agent for CXCL8 Based on a Model of the CXCL8:CXCR1 Complex.RSC Adv. 5, no. 33 (2015): 25657–68. doi:10.1039/C4RA13749C.

To see more references for this product click here.

Videos

HybriWell Seals: Rapid Setup of Reaction Chambers for Small Volumes

HybriWell Sealing System Tubing Connectors for Loading Reagents and Perfusion Applications

FAQ

Q: What is the best way to change solutions within a HybriWell™ chamber if I want to leave it affixed to the slide?

A: The simplest method to remove solutions from a HybriWell™ is to un-cap both filling ports, tip the slide vertically and allow the solution to drain.  For faster exchange or if the reagent is viscous, un-cap both filling ports and place the chambered slide into a 50ML conical tube.  Give it a quick low-speed spin (1000 rpm) in the centrifuge and all of the solution will be collected in the bottom of the tube.  This, by the way, is a great method to recover and re-use some primary antibodies or collect pre-hybe buffer to which you can add the probe, mix and load back to the chamber. 

Q: What is the difference between HybriWell™ and SecureSeal™?

A: Fundamentally, the difference between a HybriWell™ and a SecureSeal™ Chamber is the surface to volume ratio created by the differences in chamber depth.   To achieve the best active mixing,  select a chamber size in which the fluid moves freely during rotation.  Rotational mixing can be further facilitated by leaving a small “mixing bubble” within the chamber.  SecureSeals™ use greater fluid volume per surface area compared to  HybriWells™, however, to conserve reagents. HybriWells™ may be the better choice.  Both products are recommended for DNA chip slide chemistries, considering the reagents used, incubation time and temperature and surface modification of the glass, one type of chamber may bond more aggressively than the other, as they use different adhesives assemblies.

Q: I am trying to do in situ hybridization and we have 2 mouse tissue sections per slide.  Are there individual HybriWells™ where I can put one over each section on the slide?

A: Yes, we have small single-well HybriWells™, and two of them will fit on a microscope slide to create two different chambers. See our HybriWell™ 8-pack assortment (item #615101) which contains HybriWells™ of various sizes that can be adapted to your application.  Each of the HybriWells™ in the 8-pack Assortment is also sold individually in packs.

Press Fit Tubing Connectors

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Press fit tubing connectors provide affordable and easy to use means to connect Grace’s chamber products to pumps and other devices for fluidic exchange.

Adhesive Seal Tabs

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Adhesive seal tabs complement SecureSeal™ Hybridization Chambers, HybriWell™ Sealing System and CoverWell™ Perfusion Chambers. Once the paper liner is removed, the SecureSeal adhesive is exposed and the tab could easily be applied on top of a chamber’s port. A slight pressure on the adhesive tab is sufficient to create leak-proof chambers that are temperature resistant. Adhesive Seal Tabs are easily detached to allow addition or removal of reagents from the chamber. Adhesive Seal tabs are autoclavable.

Chamber Microfluidics Kit

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Press Fit Tubing Connectors and Tubing