Protein Crystallization

Protein crystallization is a key assay for structural studies of proteins. The protocols for crystallization of protein are challenging due to the stringent requirement for pure samples and control of environmental conditions during the crystallization process. Vapor diffusion using hanging drop is a preferred method for obtaining quality crystals with…

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Microarray Substrates

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Grace Bio-Labs microarray surface chemistry is based on the well-known protein-binding properties of nitrocellulose. A range of different formulations have…

APPLICATIONS:

Antigen-Capture AssayAntibody Capture AssayRPPA- Reverse Phase Protein MicroarrayLaser micro-dissection RRPAEpitope-mappingBiomarker Discovery and ValidationImmunogen Discovery

Microarray Reagents

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Grace Bio-Labs microarray regents have been specifically formulated to achieve the full potential of porous nitrocellulose, accelerating experimental design and…

APPLICATIONS:

Antigen-Capture Assay Antibody Capture Assay RPPA- Reverse Phase Protein Microarray Laser micro-dissection RRPA Epitope-mapping Biomarker Discovery and Validation Immunogen Discovery

ProPlate®

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ProPlates® were specifically designed to enable automated robotic liquid handling. Two main configurations are available: The ProPlate® Microtiter Plate is comprised…

APPLICATIONS:

ProteomicsProtein MicroarraysProtein expression analysisAntibody profiling cDNA and oligonucleotide arrays

FastWells™ Reagent Barriers

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FastWells™ are sticky, flexible silicone gaskets that form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Gaskets may…

APPLICATIONS:

MicroscopyImagingFluorescence In situ Hybridization (FISH)Single-molecule fluorescence analysisImmunohistochemistry

CoverWell™ Incubation Chambers

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CoverWell™ incubation chambers are reusable, easy to apply chambers that attach without the use of adhesive.  CoverWells™ enclose a large…

APPLICATIONS:

In-situ hybridizationImmunohistochemistryDNA MicroarrayReverse Transfection Microarray

CoverWell Perfusion Chambers

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CoverWell ™ perfusion press-to-seal covers form water-tight, multiwell cell incubation or cytochemistry chambers when pressed to coverslips or microscope slides.…

APPLICATIONS:

Live-cell imagingMicroscopyImagingSingle molecule spectroscopy

HybriSlip™ Hybridization Covers

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HybriSlips™ are rigid, light-weight, thin plastic coverslips that minimize friction and facilitate uniform reagent distribution during incubation steps which require…

APPLICATIONS:

In-situ HybridizationLorem ipsum dolorIn-situ PCRImmunohistochemistryProtein MicroarrayDNA Microarray

Imaging Spacers

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Imaging spacers are ultra-thin adhesive spacers which peel-and-stick to coverglass or microscope slides to confine specimens without compression. Layer multiple…

APPLICATIONS:

ImagingMicroscopyHigh-temperature single-molecule kinetic analysisanti‐Stokes Raman scattering microscopy

SecureSeal™ Hybridization Chambers

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SecureSeal™ Hybridization Chambers are thin, silicone-gasketed chambers providing optimal surface-to-volume fluid dynamics for hybridization assays on large or multiple specimens…

APPLICATIONS:

In situ hybridizationProtein and DNA MicroarraysImmunocytochemistryRapid microfluidic prototypingFluorescence Resonance Energy Transfer (FRET)

CoverWell™ Incubation Chambers

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CoverWell™ incubation chambers are reusable, easy to apply chambers that attach without the use of adhesive.  CoverWells™ enclose a large…

APPLICATIONS:

Reverse Transfection Microarray;  DNA Microarray;  In-situ hybridization;  Immunohistochemistry; 

Silicone Isolators

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Silicone Isolators allow researchers to isolate specimens using removable hydrophobic barriers. They may be used to isolate cells grown in…

SecureSeal™ Hybridization Chambers

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SecureSeal™ Hybridization Chambers are thin, silicone-gasketed chambers providing optimal surface-to-volume fluid dynamics for hybridization assays on large or multiple specimens…

HybriWell™ Sealing System

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HybriWell™ Sealing System bonds securely to a microscope slide surface in seconds to confine small reagent volumes with samples and…

Hybridization and Incubation

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Hybridization and incubation Seals ad Chambers from Grace Bio-Labs are ideally suited for in situ-hybridization assays. The adhesive seal of…

APPLICATIONS:

In-situ hybridization MicroarraysFluorescence In situ Hybridization (FISH)FRET (Fluorescence Resonance Energy Transfer)

HybriWell™ Sealing System 8 Pack

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Chamber 8-Pack Assortment – Includes Port Seals and Applicator

Adhesive Seal Tabs

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Adhesive seal tabs complement SecureSeal™ Hybridization Chambers, HybriWell™ Sealing System and CoverWell™ Perfusion Chambers. Once the paper liner is removed,…

APPLICATIONS:

In situ hybridization ;  Protein and DNA Microarrays ;  Immunocytochemistry ;  DNA Amplification ;  Neurophysiology ;  Reaction kinetics ; 

FastWells™ Reagent Barriers

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FastWells™ are sticky, flexible silicone gaskets that form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Gaskets may…

FlexWell™ 4 Slide Tray

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Multiple slides and FlexWells™ may be inserted into a reusable FlexWell™ tray, organizing wells into the footprint of a SBS…

APPLICATIONS:

Protein MicroarrayHybridizationIncubation

FlexWell™ Incubation Chambers

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FlexWell™ incubation chamber silicone gaskets form wells on slides using clean release adhesive to isolate up to 16 specimens per…

APPLICATIONS:

Protein MicroarrayHybridizationIncubation

FlexWell™ Seal Strips

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To prevent evaporation apply seal strips using the plastic applicator to the top surface of the chamber. Seal strips remove cleanly…

APPLICATIONS:

Microarrays using nitrocellulose coatedHybridizationIncubation

HybriSlip™ Hybridization Covers

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HybriSlips™ are rigid, light-weight, thin plastic coverslips that minimize friction and facilitate uniform reagent distribution during incubation steps which require…

ProPlates®

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ProPlates® were specifically designed to enable automated robotic liquid handling. Two main configurations are available: The ProPlate® Microtiter Plate is comprised…

APPLICATIONS:

ProteomicsProtein MicroarraysProtein expression analysis;  Antibody profiling ;  cDNA and oligonucleotide arrays; 

Silicone Isolators™ Sheet Material

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Silicone isolator™ sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. Where additional sealing is…

APPLICATIONS:

Protein and DNA arrays ;  Immunohistochemistry;  Fluorescence In situ Hybridization (FISH) ;  Biopolymers and hydrogel formulation ;  Cryogenic-transmission electron microscopy (Cryo-TEM) ;  Microwave crystallization ;  Ultra-small-angle X-ray scattering (USAXS) ;  Tissue ingeneering;  Live cell lithography” (LCL); 

Imaging Spacers

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Imaging spacers are ultra-thin adhesive spacers which peel-and-stick to coverglass or microscope slides to confine specimens without compression. Layer multiple…

APPLICATIONS:

Imaging;  Microscopy;  High-temperature single-molecule kinetic analysis;  Anti‐Stokes Raman scattering microscopy; 

CoverWell™ Imaging Chambers

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CoverWell ™ imaging chambers are designed to stabilize and support thick and free-floating specimens for confocal microscopy and imaging applications.…

APPLICATIONS:

Confocal microscopy Imaging Tissue and Cell staining ;  High Resolution Microscopy ;  Live-cell imaging ; 

CoverWell™ Perfusion Chambers

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CoverWell ™ perfusion press-to-seal covers form water-tight, multiwell cell incubation or cytochemistry chambers when pressed to coverslips or microscope slides.…

APPLICATIONS:

Single molecule spectroscopy Live-cell imaging Microscoscopy

FastWells™ Reagent Barriers

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FastWells™ are sticky, flexible silicone gaskets that form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Gaskets may…

APPLICATIONS:

Microscopy Fluorescence In situ Hybridization (FISH) Single-molecule fluorescence analysis ;  Immunohistochemistry ; 

MultiSlip™ Coverglass Inserts

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MutliSlip™ inserts with 8 (18mm x 18mm) or 15 (12mm x 12mm) No. 1.5 German glass coverglass per insert are…

APPLICATIONS:

High resolution microscopy Fluorescent imaging Immunohistochemistry ;  Cell Culture; 

SecureSeal™ Adhesive Sheets

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These adhesive sheets are made using the same SecureSeal™ adhesive as is used to make HybriWell™ and SecureSeal™ Incubation Chambers.  Thin,…

APPLICATIONS:

Imaging ;  Tissue and Cell staining ;  High Resolution Microscopy; 

SecureSlip™ Silicone Supported Coverglass

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SecureSlip™ Silicone Supported Coverglass is affixed to a thin microscopically transparent silicone base which secures it to culture vessels by…

Imaging

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Imaging seals and chambers from Grace Bio-Labs offer a selection of tools for cell/tissue staining for high quality results in…

APPLICATIONS:

Tissue and Cell stainingHigh Resolution MicroscopyLive-cell imaging

Adhesive Seal Tabs

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Adhesive seal tabs complement SecureSeal™ Hybridization Chambers, HybriWell™ Sealing System and CoverWell™ Perfusion Chambers. Once the paper liner is removed,…

APPLICATIONS:

In situ hybridization ;  Protein and DNA Microarrays;  Immunocytochemistry ;  DNA Amplification ;  Neurophysiology ;  Reaction kinetics; 

CultureWell™ Coverglass Removal Tool

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CS16 CultureWell™ removable chambered coverglass is a 16-well chambered coverglass cell culture vessel, with 2 x 8 format with standard…

APPLICATIONS:

Cell culture Fluorescence imaging

CultureWell™ Microplate Adapter Tray

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CultureWell™ Microplate Adapter Tray holds up to 4 CultureWell™ 16 Chambers for higher throughput

CultureWell™ MultiWell Chambered Coverslips

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CultureWell™ chambered coverglass products consist of removable and reusable, non-cytotoxic silicone gaskets secured to number 1.5 German coverglass. Chambered coverglass…

APPLICATIONS:

Cell Culture Fluorescence applications In-situ hybridization Immunostaining

CS16-CultureWell™ Removable Chambered Coverglass

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CS16 CultureWell™ removable chambered coverglass is a 16-well chambered coverglass cell culture vessel, with 2 x 8 format with standard…

APPLICATIONS:

Cell CultureFluorescence applicationsIn-situ hybridizationImmunostaining

CultureWell™ Coverglass Inserts

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Each CultureWell™ coverglass insert is comprised of four chambered coverglass, assembled in a disposable frame placed in a standard 86mm…

APPLICATIONS:

High resolution microscopy Fluorescent imaging Immunohistochemistry

CultureWell™ Reusable Gaskets

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Gaskets are ideal for forming wells on glass microscope slides or in polystyrene dishes. Gaskets are non-sterile and may be…

APPLICATIONS:

Cell CultureHigh resolution microscopyFluorescent imaging Immunohistochemistry

CultureWell™ Silicone Sheet Material

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CultureWell™ clear silicone sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. They may be…

APPLICATIONS:

Cell CultureHigh resolution microscopy Fluorescent imagingImmunohistochemistry

MultiSlip™ Coverglass Inserts

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MutliSlip™ inserts with 8 (18mm x 18mm) or 15 (12mm x 12mm) No. 1.5 German glass coverglass per insert are…

APPLICATIONS:

Cell CultureFluorescent imaging Immunohistochemistry

SecureSlip™ Silicone Supported Coverglass

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SecureSlip™ Silicone Supported Coverglass is affixed to a thin microscopically transparent silicone base which secures it to culture vessels by…

APPLICATIONS:

Cell CultureImmunofluorescence assayMicroscopy

CultureWell™ ChamberSLIP 16, Non-Removable Chambered Coverglass

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CultureWell™ NON Removable Chambered Coverglass, 16 Well, No. 1.5 German borosilicate Coverglass. Product consists of cell culture vessels, with a…

APPLICATIONS:

Cell Culture Fluorescence applicationsSmall volume incubation Immunostaining

Silicone Wound Splints

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Wound splints are constructed of silicone and include suture sites for increased precision in affixing on or within an animal…

Silicone Isolator Sheet Material

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Silicone isolator™ sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. Where additional sealing is…

APPLICATIONS:

Protein and DNA arrays ;  Immunohistochemistry ;  Fluorescence In-situ Hybridization (FISH) ;  Biopolymers and hydrogel formulation;  Cryogenic-transmission electron microscopy (Cryo-TEM) ;  X-ray scattering ;  Microwave crystallization ;  Ultra-small-angle X-ray scattering (USAXS) ;  Tissue engineering Live cell lithography (LCL); 

CultureWell Silicone Sheet Material

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CultureWell™ clear silicone sheet material allows researchers to create their own removable hydrophobic barriers to isolate specimens. They may be…

APPLICATIONS:

Lorem Ipsum ;  Lorem Ipsum;  Lorem Ipsum; 

HybriSlip™ Hybridization Covers

HYBRISLIP™ HYBRIDIZATION COVERS

HybriSlip™ hybridization covers are rigid, light-weight, thin plastic coverslips that minimize friction and facilitate uniform reagent distribution during incubation steps which require small reagent volume.

HybriSlips™ are RNase and DNase free, hydrophobic covers that will not trap or bind probes to their surfaces like coverglass.   Working surfaces are protected from nuclease contamination by clean liners and are ready to use without pretreatment.   HybriSlips™ remain flat and unlike Parafilm, will not curl, even at high temperatures.   HybriSlips™ are clear, will not chip or break, and are lighter weight than glass, minimizing friction and facilitating uniform reagent distribution.

HybriSlips™ are easily customized providing users with options tailored to suit their application.  Changes in size, shape and quantity per package are the most common requests.  HybriSlip™ plastic material is also stocked in thickness 0.18 mm.

Hybrislips™ are manufactured from rigid, nuclease free polycarbonate plastic which has a nominal thickness of 0.254 mm.  Both surfaces are protected with a thin, removable layer of polyethylene film to prevent contamination.  HybriSlips™ are packaged in quantities of 100 per bag in strips of 5 each. This product is available in a wide variety of square or rectangular dimensions and is easily customized to fit your specific application requirements.

Ideally suited for incubation steps where reagent volume is limited such as:  In situ hybridization, in situPCR, microarray hybridizations and immunohistochemistry. For best results, incubation steps should be performed in a humidified chamber to minimize evaporation from the edges. Where CV’s between replicate samples are important, distributing the same volume of reagent per cm2 can be a critical variable. HybriSlips™ are designed to overcome the limitations of do-it-yourself methods that involve trimming plastic films as covers.

Applications

  • DNA Microarray
  • Protein Microarray
  • Immunohistochemistry
  • In-situ Hybridization
  • In-situ PCR

References

Ducreux, L. J. M., Morris, W. L., Hedley, P. E., Shepherd, T., Davies, H. V., Millam, S., & Taylor, M. A. (2005). Metabolic engineering of high carotenoid potato tubers containing enhanced levels of beta-carotene and lutein. Journal of Experimental Botany, 56(409), 81–89.

Savagner, P., Kusewitt, D. F., Carver, E. A., Magnino, F., Choi, C., Gridley, T., & Hudson, L. G. (2005). Developmental transcription factor slug is required for effective re-epithelialization by adult keratinocytes. Journal of Cellular Physiology, 202(3), 858–866. 

Christianson, S., Golding, G. R., Campbell, J., Canadian Nosocomial Infection Surveillance Program, & Mulvey, M. R. (2007). Comparative genomics of Canadian epidemic lineages of methicillin-resistant Staphylococcus aureus. Journal of Clinical Microbiology, 45(6), 1904–1911. 

To see more references related to this product click here.

FAQ

Q:  My hybridization cocktail contains Formamide , will HybriSlips™ work with this reagent?

A:  Yes, HypriSlips™ are chemically resistant to most commonly used hybridization reagents.  For a full list of chemical compatibilities you can consult many of the tables that are available on-line and search: polycarbonate.

Q:  What is the best method to use for removing  a HybriSlip™ from atop my specimen?

A:   To minimize the surface tension between the HybriSlip™ and your specimen, we recommend dipping slides into a dish contain wash buffer and allowing the HybriSlip™ to slide off gently.  Peeling them off, just as with glass coverslips, is not recommended as this can compromise sample adherence and morphology.

Q:  How can I sterilize our HybriSlips™?

A:  Using sterile technique, remove the liners from both surfaces of the product followed by one of the following options:  dipping them into 70% isopropanol  and allowing them to dry,  using  UV sterilization in the hood, or lightly flaming them and allowing them to cool before use.  You may also autoclave the HybriSlips™ – first remove the liners (they melt).  

Q: What is the refractive index of the polycarbonate?

A:  The refractive index of the polycarbonate used to manufacture HybriSlips™  is 1.586

Q: What is the working temperature range for HybriSlip™ material?

A:  Materials remain stable between  minus  135 to 121oC

CoverWell™ Incubation Chambers

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CoverWell™ incubation chambers are reusable, easy to apply chambers that attach without the use of adhesive.  CoverWells™ enclose a large sample area with a small reagent volume and preserve kinetic (non-capillary) fluid dynamics for better reagent mixing and lower backgrounds within the chamber for more uniformly sensitive assays. These ready-to-use chambers are designed expressly for in situ hybridization and immunocytochemistry.

FastWells™ Reagent Barriers

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FastWells™ are sticky, flexible silicone gaskets that form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Gaskets may be stacked to increase depth and volume. FastWell™ Reagent Barriers provide rapid isolation of cells and tissues on slides or coverslips for antibody incubations. The barriers remain sealed during agitated washing steps, preventing reagents from spilling when shaken or rocked. FastWells™ peel off quickly and cleanly, leaving no residue to interfere with specimen cover-slipping. They can be washed, autoclaved and reused. The barriers can also be sealed to form incubation chambers using flexible HybriSlip™ covers.

FlexWell™ 4 Slide Tray

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Multiple slides and FlexWells™ may be inserted into a reusable FlexWell™ tray, organizing wells into the footprint of a SBS compliant microtiter plate to facilitate handling.

FlexWell™ Incubation Chambers

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FlexWell™ incubation chamber silicone gaskets form wells on slides using clean release adhesive to isolate up to 16 specimens per slide. Slides fit into a reusable slide tray organizing wells into the footprint of a microtiter plate. Gaskets may be sealed for mixing and to prevent evaporation.

FlexWell™ Seal Strips

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To prevent evaporation apply seal strips using the plastic applicator to the top surface of the chamber. Seal strips remove cleanly and easily without leaving a residue.

HybriWell™ Sealing System

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HybriWell™ Sealing System bonds securely to a microscope slide surface in seconds to confine small reagent volumes with samples and eliminate evaporation. Each package includes illustrated instructions for use, applicator and 200 adhesive port seals. Any of the designs listed may be easily customized to increase or decrease chamber depth. Hybriwells™ are recommended for most protein and nucleic acid assays. If you are using Cy5 or ® Alexa Fluor 647 direct-labeled DNA probes please refer to our new “fluorescent friendly” chambers. All current designs may be fabricated using our "fluorescent friendly" adhesive material.

ProPlate®

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ProPlates® were specifically designed to enable automated robotic liquid handling. Two main configurations are available:

  • The ProPlate® Microtiter Plate is comprised of a SBS compliant microtiter plate superstructure and feature a SesureSeal ™ adhesive bonding system applied to the bottom. Click here for more information
  • The ProPlate® Multi –Array Slide System integrates microscope array technology with automated microtiter plate processing. Individual modules fit into a tray with a standard microtiter plate footprint. Click here for more information

SecureSeal™ Hybridization Chambers

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SecureSeal™ Hybridization Chambers are thin, silicone-gasketed chambers providing optimal surface-to-volume fluid dynamics for hybridization assays on large or multiple specimens and microarrays on glass or film coated slides. These adhesive-backed chambers are designed for single-use, very-low volume incubations and are ideal for autoradiographic, fluorescent or chemiluminescent end-point applications requiring small, enclosed fluid volumes. SecureSeal™ Adhesive is a double sided tape with a silicone adhesive surface on one side (covered with a clear PET liner) and an acrylic adhesive on the other surface (covered with a paper liner).

Silicone Isolators™

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Silicone Isolators allow researchers to isolate specimens using removable hydrophobic barriers. They may be used to isolate cells grown in culture dishes or separate multiple specimens affixed to microscope slides. Isolators are available in red silicone thickness 0.5 to 2.5mm pre-cut or as sheet material with or without secure seal clean adhesive on one or both surfaces. Nonadhesive isolators are autoclavable and reusable.Silicone Isolators™ remain sealed to smooth surfaces during washing steps. Where additional sealing is required, SecureSeal™ adhesive on one or both surfaces is recommended.