FastWells™ Reagent Barriers
FASTWELLS™ REAGENT BARRIERS
FastWells™ Reagent Barriers are sticky, flexible silicone gaskets that form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Gaskets may be stacked to increase depth and volume. FastWell™ Reagent Barriers provide rapid isolation of cells and tissues on slides or coverslips for antibody incubations. The barriers remain sealed during agitated washing steps, preventing reagents from spilling when shaken or rocked. FastWells™ peel off quickly and cleanly, leaving no residue to interfere with specimen. They can be washed, autoclaved, and reused. The barriers can also be sealed to form incubation chambers using flexible HybriSlip™ covers.
Applications
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Microscopy
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Fluorescence In situ Hybridization (FISH)
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Single-molecule fluorescence analysis
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Immunohistochemistry
Videos
Silicone Isolator Application
References
Yokomizo, T., & Dzierzak, E. (2010). Three-dimensional cartography of hematopoietic clusters in the vasculature of whole mouse embryos. Development, 137(21), 3651–3661.
Yokomizo, T., Yamada-Inagawa, T., Yzaguirre, A. D., Chen, M. J., Speck, N. A., & Dzierzak, E. (2012). Whole-mount three-dimensional imaging of internally localized immunostained cells within mouse embryos. Nature Protocols, 7(3), 421–431.
Wang, Y.-H., Collins, A., Guo, L., Smith-Dupont, K. B., Gai, F., Svitkina, T., & Janmey, P. A. (2012). Divalent Cation-Induced Cluster Formation by Polyphosphoinositides in Model Membranes. Journal of the American Chemical Society, 134(7), 3387–3395.
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Silicone Isolators allow researchers to isolate specimens using removable hydrophobic barriers. They may be used to isolate cells grown in culture dishes or separate multiple specimens affixed to microscope slides. Isolators are available in red silicone thickness 0.5 to 2.5mm pre-cut or as sheet material with or without secure seal clean adhesive on one or both surfaces. Nonadhesive isolators are autoclavable and reusable.Silicone Isolators™ remain sealed to smooth surfaces during washing steps. Where additional sealing is required, SecureSeal™ adhesive on one or both surfaces is recommended.
