HYBRIDIZATION AND INCUBATION
Grace Bio-Labs’ hybridization and incubation chambers isolate reagents and specimens on microscope slides or coverslips. Several different designs and configurations are available to meet the needs of your experimental settings. Any experimental protocol requiring an incubation step could be expedited and simplified by selecting the appropriate chamber. Check our selection guide or contact us if you need help selecting the right tool for your application.
HybriWell™ Sealing System bonds securely to a microscope slide surface in seconds to confine small reagent volumes with samples and eliminate evaporation. Each package includes illustrated instructions for use, applicator and 200 adhesive port seals. Any of the designs listed may be easily customized to increase or decrease chamber depth. Hybriwells™ are recommended for most protein and nucleic acid assays. If you are using Cy5 or ® Alexa Fluor 647 direct-labeled DNA probes please refer to our new “fluorescent friendly” chambers. All current designs may be fabricated using our "fluorescent friendly" adhesive material.
SecureSeal™ Hybridization Chambers are thin, silicone-gasketed chambers providing optimal surface-to-volume fluid dynamics for hybridization assays on large or multiple specimens and microarrays on glass or film coated slides. These adhesive-backed chambers are designed for single-use, very-low volume incubations and are ideal for autoradiographic, fluorescent or chemiluminescent end-point applications requiring small, enclosed fluid volumes. SecureSeal™ Adhesive is a double sided tape with a silicone adhesive surface on one side (covered with a clear PET liner) and an acrylic adhesive on the other surface (covered with a paper liner).
Adhesive seal tabs complement SecureSeal™ Hybridization Chambers, HybriWell™ Sealing System and CoverWell™ Perfusion Chambers. Once the paper liner is removed, the SecureSeal adhesive is exposed and the tab could easily be applied on top of a chamber’s port. A slight pressure on the adhesive tab is sufficient to create leak-proof chambers that are temperature resistant. Adhesive Seal Tabs are easily detached to allow addition or removal of reagents from the chamber. Adhesive Seal tabs are autoclavable.
