PATH® Protein Microarray Slide

PATH® Protein Microarray Slide, Barcoded – 20 PACK

PATH® is a registered trademark of Courtagen Life Sciences and manufactured exclusively by Grace Bio-Labs, Inc.

Protein Microarray Substrates Selection Guides

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Property

Oncyte® Brand Nitrocellulose Thin Film Slides

Non-porous
Nitrocellulose Film*

Avid

Nova

SuperNova

PATH®

Binding capacity

Film thickness

12 µm

7 µm

12 µm

< 200 nm

Dynamic Range
(log scale fluorescence)

5-6

5-6

7+

4-5

Hydrophobicity1
(contact angle)

80°

70°

60°

70°

Preferred Detection
Methods 2

Colorimetric,
Chemiluminescence

Fluorescence

Fluorescence

Fluorescence

Applications

Antigen or antibody binding, cell or tissue lysate binding, glycoprotein and peptide binding assays

Antigen or antibody binding, glycoprotein and peptide binding assays

Antigen or antibody binding cell or tissue lysate binding glycoprotein and peptide binding assay

Antigen or antibody binding, glycoprotein and peptide binding assays

*   Exclusively from Grace Bio-Labs
1   More hydrophobic surfaces may result in reduced spot diameter, depending on the spotting buffer composition. Results may vary based on buffers, sample preparation, spotting and scanning instruments. See our ONCYTE® user guide for full discussion.
2   All detection methods are compatible with ONCYTE® Nitrocellulose Film Slides.

References

Pollard, H. B., Srivastava, M., Eidelman, O., Jozwik, C., Rothwell, S. W., Mueller, G. P., … Paweletz, C. P. (2007). Protein microarray platforms for clinical proteomics. PROTEOMICS – Clinical Applications, 1(9), 934–952. https://doi.org/10.1002/prca.200700154
 
Gyorgy, A. B., Walker, J., Wingo, D., Eidelman, O., Pollard, H. B., Molnar, A., & Agoston, D. V. (2010). Reverse phase protein microarray technology in traumatic brain injury. Journal of Neuroscience Methods, 192(1), 96–101. https://doi.org/10.1016/j.jneumeth.2010.07.029
 
Cao, Z., Partyka, K., McDonald, M., Brouhard, E., Hincapie, M., Brand, R. E., … Haab, B. B. (2013). Modulation of Glycan Detection on Specific Glycoproteins by Lectin Multimerization. Analytical Chemistry, 85(3), 1689–1698. https://doi.org/10.1021/ac302826a
 

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