HybriWell™ Sealing System
8-Pack Assortment – Includes Port Seals and Applicator – EACH
HybriWell™ Sealing System bonds securely and rapidly to a microscope slide surface to confine small reagent volumes with samples. HybriWells™ are manufactured using a clear plastic polycarbonate cover (0.010”- 0.254mm thick) and double-sided adhesives (chamber). Two access ports (1.5mm diameter) are added to each chamber to allow for the addition or removal of reactants. Ports are easily sealed using Adhesive Seal Tabs (200 tabs are included), see the video. Sealed chambers are watertight and ideally suited for submerged or overnight incubations. RNase free, hydrophobic surfaces will not trap or bind probes. Press fit tubing connectors provide affordable and easy means to connect HybriWells™ to pumps and other devices for fluidic exchange.
If you are using Cy5 or ® Alexa Fluor 647 direct-labeled DNA probes please refer to our new “fluorescent friendly” chambers, see Fluorescent Friendly adhesive tab for more information. All current designs may be fabricated using our “fluorescent friendly” adhesive material.
Chamber depth and dimensions could easily be customized, a variety of adhesive options are also available for applications that employ the use of “exotic” surface chemistries. Grace Bio-Labs, has a large portfolio of adhesive options, plastics, and know-how to tailor a solution to fit your needs.
Fluorescent Friendly Adhesive
Standard versions of the HybriWell™ are manufactured using double-sided, clear SecureSeal™ Adhesive. The Fluorescent Friendly Adhesive (black) was developed in response to reports from few laboratories that they were experiencing signal quenching using CY5 and AF647 using SecureSeal™ Adhesive. We contacted multiple HybriWell™ customers using these fluors for hybridization and results were mixed with many of the labs having no issues whatsoever. After creating a matrix of variables, the information we know to date is;
- The phenomenon only occurs during hybridization assays above 55C
- Only occurs if the fluor is directly conjugated to the probe (no problem in a sandwich assay).
- CY3 and other fluors are not affected.
- Some labs do not have the quenching issue at all under any of the above conditions.
As far as we know, some component of the hybridization buffer is reacting with some component of the adhesive used to make standard HybriWell™ and SecureSeal™ products resulting in signal quenching. We are not able to identify which component may be the culprit as many of the customers having the problem were using pre-made hybe buffers, the manufacturers of which will not share the formulation with us. We feel that it is appropriate to alert custom