HybriSlip™ Hybridization Covers

HybriSlip™ Hybridization Cover-HS2274CS, 22mm X 74mm X 0.25mm Thickness – 1000 CASE

Technical Resources

Instructions

Instructions for Use HybriSlip Hybridization Covers

Applications

  • DNA Microarray

  • Protein Microarray

  • Immunohistochemistry

  • In-situ Hybridization

  • In-situ PCR

 

 

References

Ducreux, L. J. M., Morris, W. L., Hedley, P. E., Shepherd, T., Davies, H. V., Millam, S., & Taylor, M. A. (2005). Metabolic engineering of high carotenoid potato tubers containing enhanced levels of beta-carotene and lutein. Journal of Experimental Botany, 56(409), 81–89.

Savagner, P., Kusewitt, D. F., Carver, E. A., Magnino, F., Choi, C., Gridley, T., & Hudson, L. G. (2005). Developmental transcription factor slug is required for effective re-epithelialization by adult keratinocytes. Journal of Cellular Physiology, 202(3), 858–866. 

Christianson, S., Golding, G. R., Campbell, J., Canadian Nosocomial Infection Surveillance Program, & Mulvey, M. R. (2007). Comparative genomics of Canadian epidemic lineages of methicillin-resistant Staphylococcus aureus. Journal of Clinical Microbiology, 45(6), 1904–1911. 

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FAQ

Q:  My hybridization cocktail contains Formamide , will HybriSlips™ work with this reagent?

A:  Yes, HypriSlips™ are chemically resistant to most commonly used hybridization reagents.  For a full list of chemical compatibilities you can consult many of the tables that are available on-line and search: polycarbonate.

Q:  What is the best method to use for removing  a HybriSlip™ from atop my specimen?

A:   To minimize the surface tension between the HybriSlip™ and your specimen, we recommend dipping slides into a dish contain wash buffer and allowing the HybriSlip™ to slide off gently.  Peeling them off, just as with glass coverslips, is not recommended as this can compromise sample adherence and morphology.

Q:  How can I sterilize our HybriSlips™?

A:  Using sterile technique, remove the liners from both surfaces of the product followed by one of the following options:  dipping them into 70% isopropanol  and allowing them to dry,  using  UV sterilization in the hood, or lightly flaming them and allowing them to cool before use.  You may also autoclave the HybriSlips™ – first remove the liners (they melt).  

Q: What is the refractive index of the polycarbonate?

A:  The refractive index of the polycarbonate used to manufacture HybriSlips™  is 1.586

Q: What is the working temperature range for HybriSlip™ material?

A:  Materials remain stable between  minus  135 to 121oC