DNA Microarray slides

Epoxy Microarray Slides -w/Barcodes – L x W x H – 75.5 x 25.2 x 1.0 – 25 Pack

Epoxy Microarray Slides w/Barcodes

Epoxy Microarray Slides w/Barcodes provide a uniform substrate for a variety of DNA/RNA-based diagnostic applications. The epoxy-activated glass is well documented as a reliable and efficient surface in microarray methods. Biomolecules containing amino, hydroxy, or thiol groups are highly reactive with epoxide functional groups. Covalent linkage delivers a robust immobilization technique for quantitative assays and reproducible spots. Compatible with both non-contact and contact printers, hybridization chambers, and fluorescence-based detection systems. Slides are produced under cleanroom conditions to generate a homogenous coating and preserve the low background fluorescence of glass slides.

Printed Labeled DNA Oligos for Epoxysilane

Print Process

• Hardware: Scienion sciFLEXARRAYER S3 with PDC 70 Type 1
• Spot Volume: 600 pL
• Capture Probe: 18nt DNA Oligo +3′ Cy3 +5′ Am-6C linker; Serial dilutions
• Print Buffer: 3x SSC + 1.5M Betaine, pH 9
• Preprint Condition: 75% RH, 20°C, 30 min
• Print Condition: 75% RH, 20°C, 30 min

Post Print Process

• Incubate: Saturated humidity, 60°C, 1 hour
• GBL SuperG™ Blocking Buffer, spray coat 4x and immersion 1x for 10 min
• Wash: 2x SSC +0.1% SDS, 10 minutes gentle agitation
• Wash: 0.2x SSC, 1 minute gentle agitation
• Wash: 0.1x SSC, 1 minute gentle agitation
• Dry: Centrifuge, 10 minutes, air dry >30 minutes

Scan and Analyze

• Hardware: Innopsys Innoscan AL-1100
• Settings: Low power, PMT gain 2, Resolution 5µm
• Analysis Software: Innopsys Mapix using auto spot location and size features; intensity displayed with rainbow color scheme(red: high; blue: mid: black: low)
• Presentation software: Python custom code to generate graphs and statistical information

Epoxysilane Slides with IgG 2x Dilutions

Print Process

• Hardware: Scienion sciFLEXARRAYER S3 with PDC 70 Type 4
• Sample Prep: IgG – Alexa Fluor™ 647; Serial dilutions
• Print Buffer: 4% GBL Protein Array Printing Buffer
• Conditions: 70% RH, 20°C, incubated for 10 hours

Post Print Process

• Block: GBL SuperG™ Blocking Buffer, spray coat 4x and immersion 1x for 10 min
• Wash: PBST, 5 minutes gentle agitation, 3x
• Rinse: D1 water submersion, 1 min gentle agitation, 3x
• Dry: Centrifuge, 10 minutes

Scan and Analyze

• Hardware: Innopsys Innoscan 1100
• Settings: Low power, PMT gain 10, Resolution 5μm
• Analysis Software: Innopsis Mapix using auto spot location and size features; intesity displayed with rainbow color scheme(red:high; blue: mid: black: low)
• Presentation software: Python custom code to generate graphs and statistical information

Scan of Grace Biolabs Epoxysilane slide with IgG Dilution series