Chamber Microfluidics Kit

Microfluidics Chamber Kit – Press Fit Tubing Connectors, 40 each and Tubing, 5 m Length, PTFE, Translucent – 1 EACH

The kit comprises Press Fit Tubing Connectors and tubing for fluidic applications. The press fit tubing connectors are designed to provide an easy and rapid mean to connect Grace Bio-Labs chambers to pumps or syringes.

Simply apply connectors by pressing the adhesive coated surface to the filling ports on the chamber surface and insert the appropriate length of tubing through the opening on the top. The simple hourglass design through the wall of the connector tightly secures the tubing to form a leak-proof seal in communication with the chamber.  The open end of the tubing may be connected to a syringe or pump using a 25 gauge cannula or needle.

 

 

Technical Resources

Instructions

Instructions for Use Press Fit Tubing Connectors

Applications

  • Fluorescence Resonance Energy Transfer (FRET)
  • Single molecule FRET
  • Prototyping of microfluidic systems
  • Imaging
  • Live-cell imaging

References

Ramaswamy, S., Cooper, D., Poddar, N., MacLean, D. M., Rambhadran, A., Taylor, J. N., … Jayaraman, V. (2012). Role of Conformational Dynamics in α-Amino-3-hydroxy-5-methylisoxazole-4-propionic Acid (AMPA) Receptor Partial Agonism. Journal of Biological Chemistry, 287(52), 43557–43564. https://doi.org/10.1074/jbc.M112.371815
 
Khoury, C., Mensing, G. A., & Beebe, D. J. (2002). Ultra rapid prototyping of microfluidic systems using liquid phase photopolymerization. Lab on a Chip, 2(1), 50–55. https://doi.org/10.1039/B109344D
 
Dolino, D. M., Cooper, D., Ramaswamy, S., Jaurich, H., Landes, C. F., & Jayaraman, V. (2015). Structural Dynamics of the Glycine-binding Domain of the N-Methyl-d-Aspartate Receptor. Journal of Biological Chemistry, 290(2), 797–804. https://doi.org/10.1074/jbc.M114.605436
 
Cooper, D., Uhm, H., Tauzin, L. J., Poddar, N., & Landes, C. F. (2013). Photobleaching Lifetimes of Cyanine Fluorophores Used for Single-Molecule Förster Resonance Energy Transfer in the Presence of Various Photoprotection Systems. ChemBioChem, 14(9), 1075–1080. https://doi.org/10.1002/cbic.201300030

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