Press Fit Tubing Connectors
Press fit tubing connectors provide affordable and easy to use means to connect Grace’s chamber products to pumps and other devices for fluidic exchange.
Simply apply connectors by pressing the adhesive coated surface to the filling ports on the chamber surface and insert the appropriate length of #24 gauge tubing through the opening on the top. The simple hourglass design through the wall of the connector, tightly secures the tubing to form a leak-proof seal in communication with the chamber. The open end of the tubing may be connected to a syringe or pump using a 25 gauge cannula or needle.
Applications
- Fluorescence Resonance Energy Transfer (FRET)
- Single molecule FRET
- Prototyping of microfluidic systems
- Imaging
- Live-cell imaging
References
Videos
Adhesive seal tabs complement SecureSeal™ Hybridization Chambers, HybriWell™ Sealing System and CoverWell™ Perfusion Chambers. Once the paper liner is removed, the SecureSeal adhesive is exposed and the tab could easily be applied on top of a chamber’s port. A slight pressure on the adhesive tab is sufficient to create leak-proof chambers that are temperature resistant. Adhesive Seal Tabs are easily detached to allow addition or removal of reagents from the chamber. Adhesive Seal tabs are autoclavable.
CoverWell ™ perfusion press-to-seal covers form water-tight, multiwell cell incubation or cytochemistry chambers when pressed to coverslips or microscope slides. Reagents can be quickly added and removed through dual access ports without disturbing or cross-contaminating specimens. Chambers are available in five chamber configurations allowing up to eight different specimens or analytical tests on one coverglass.
HybriWell™ Sealing System bonds securely to a microscope slide surface in seconds to confine small reagent volumes with samples and eliminate evaporation. Each package includes illustrated instructions for use, applicator and 200 adhesive port seals. Any of the designs listed may be easily customized to increase or decrease chamber depth. Hybriwells™ are recommended for most protein and nucleic acid assays. If you are using Cy5 or ® Alexa Fluor 647 direct-labeled DNA probes please refer to our new “fluorescent friendly” chambers. All current designs may be fabricated using our "fluorescent friendly" adhesive material.
SecureSeal™ Hybridization Chambers are thin, silicone-gasketed chambers providing optimal surface-to-volume fluid dynamics for hybridization assays on large or multiple specimens and microarrays on glass or film coated slides. These adhesive-backed chambers are designed for single-use, very-low volume incubations and are ideal for autoradiographic, fluorescent or chemiluminescent end-point applications requiring small, enclosed fluid volumes. SecureSeal™ Adhesive is a double sided tape with a silicone adhesive surface on one side (covered with a clear PET liner) and an acrylic adhesive on the other surface (covered with a paper liner).
