Imaging Spacers
IMAGING SPACERS
Imaging spacers are ultra-thin adhesive spacers which peel-and-stick to coverglass or microscope slides to confine specimens without compression. Layer multiple spacers to custom build chambers to any depth desired.
Thin, double-sided adhesive spacers confine specimens without compression. For high resolution microscopy, sandwich specimen and spacer between two No. 0 coverglass. Imaging spacers can be fabricated in different material and thickness for specific applications. The adhesive, also used to make Wound Splints, HybriWell and SecureSeal incubation chambers, is now available in sheets that may be purchased in a single layer thickness or prepared in multiple layers for your convenience. Please visit the SecureSeal™ adhesive sheet for more information.
If you need a thicker option see our Silicone Isolators and Silicone isolator™ sheet material
Applications
- Imaging
- Microscopy
- High-temperature single-molecule kinetic analysis
- anti‐Stokes Raman scattering microscopy
References
Greenfeld, C. R., Pepling, M. E., Babus, J. K., Furth, P. A., & Flaws, J. A. (2007). BAX regulates follicular endowment in mice. Reproduction (Cambridge, England), 133(5), 865–876.
Saylor, K. W. (2010). Developmental regulation of fascin and plastin actin crosslinkers in the chicken basilar papilla. Scholar Archive.
Campbell, J. D., McDonough, J. E., Zeskind, J. E., Hackett, T. L., Pechkovsky, D. V., Brandsma, C.-A., … Spira, A. (2012). A gene expression signature of emphysema-related lung destruction and its reversal by the tripeptide GHK. Genome Medicine, 4(8), 67.
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CoverWell ™ perfusion press-to-seal covers form water-tight, multiwell cell incubation or cytochemistry chambers when pressed to coverslips or microscope slides. Reagents can be quickly added and removed through dual access ports without disturbing or cross-contaminating specimens. Chambers are available in five chamber configurations allowing up to eight different specimens or analytical tests on one coverglass.
CoverWell™ incubation chambers are reusable, easy to apply chambers that attach without the use of adhesive. CoverWells™ enclose a large sample area with a small reagent volume and preserve kinetic (non-capillary) fluid dynamics for better reagent mixing and lower backgrounds within the chamber for more uniformly sensitive assays. These ready-to-use chambers are designed expressly for in situ hybridization and immunocytochemistry.
FastWells™ are sticky, flexible silicone gaskets that form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Gaskets may be stacked to increase depth and volume. FastWell™ Reagent Barriers provide rapid isolation of cells and tissues on slides or coverslips for antibody incubations. The barriers remain sealed during agitated washing steps, preventing reagents from spilling when shaken or rocked. FastWells™ peel off quickly and cleanly, leaving no residue to interfere with specimen cover-slipping. They can be washed, autoclaved and reused. The barriers can also be sealed to form incubation chambers using flexible HybriSlip™ covers.
HybriSlips™ are rigid, light-weight, thin plastic coverslips that minimize friction and facilitate uniform reagent distribution during incubation steps which require small reagent volume. HybriSlips™ are RNase and DNase free, hydrophobic covers that will not trap or bind probes to their surfaces like coverglass. Working surfaces are protected from nuclease contamination by clean liners and are ready to use without pretreatment. HybriSlips™ remain flat and unlike Parafilm, will not curl, even at high temperatures. HybriSlips™ are clear, will not chip or break, and are lighter weight than glass, minimizing friction and facilitating uniform reagent distribution.
HybriWell™ Sealing System bonds securely to a microscope slide surface in seconds to confine small reagent volumes with samples and eliminate evaporation. Each package includes illustrated instructions for use, applicator and 200 adhesive port seals. Any of the designs listed may be easily customized to increase or decrease chamber depth. Hybriwells™ are recommended for most protein and nucleic acid assays. If you are using Cy5 or ® Alexa Fluor 647 direct-labeled DNA probes please refer to our new “fluorescent friendly” chambers. All current designs may be fabricated using our "fluorescent friendly" adhesive material.
Grace Bio-Labs microarray regents have been specifically formulated to achieve the full potential of porous nitrocellulose, accelerating experimental design and data collection and validation.
Grace Bio-Labs microarray surface chemistry is based on the well-known protein-binding properties of nitrocellulose. A range of different formulations have been developed to provide optimal solutions for all protein microarray applications.
SecureSeal™ Hybridization Chambers are thin, silicone-gasketed chambers providing optimal surface-to-volume fluid dynamics for hybridization assays on large or multiple specimens and microarrays on glass or film coated slides. These adhesive-backed chambers are designed for single-use, very-low volume incubations and are ideal for autoradiographic, fluorescent or chemiluminescent end-point applications requiring small, enclosed fluid volumes. SecureSeal™ Adhesive is a double sided tape with a silicone adhesive surface on one side (covered with a clear PET liner) and an acrylic adhesive on the other surface (covered with a paper liner).
Silicone Isolators allow researchers to isolate specimens using removable hydrophobic barriers. They may be used to isolate cells grown in culture dishes or separate multiple specimens affixed to microscope slides. Isolators are available in red silicone thickness 0.5 to 2.5mm pre-cut or as sheet material with or without secure seal clean adhesive on one or both surfaces. Nonadhesive isolators are autoclavable and reusable.Silicone Isolators™ remain sealed to smooth surfaces during washing steps. Where additional sealing is required, SecureSeal™ adhesive on one or both surfaces is recommended.
These adhesive sheets are made using the same SecureSeal™ adhesive as is used to make HybriWell™ and SecureSeal™ Incubation Chambers. Thin, double-sided tape on a non-woven carrier. Clear, polyester liner on one surface, white clay coated paper on the other. SecureSeal™ Sheets consist of various thickness to choose from. These double sided adhesive sheets measure 7 x 10 inches.
