HybriSlip™ Hybridization Covers
HYBRISLIP™ HYBRIDIZATION COVERS
HybriSlip™ hybridization covers are rigid, light-weight, thin plastic coverslips that minimize friction and facilitate uniform reagent distribution during incubation steps which require small reagent volume.
HybriSlips™ are RNase and DNase free, hydrophobic covers that will not trap or bind probes to their surfaces like coverglass. Working surfaces are protected from nuclease contamination by clean liners and are ready to use without pretreatment. HybriSlips™ remain flat and unlike Parafilm, will not curl, even at high temperatures. HybriSlips™ are clear, will not chip or break, and are lighter weight than glass, minimizing friction and facilitating uniform reagent distribution.
HybriSlips™ are easily customized providing users with options tailored to suit their application. Changes in size, shape and quantity per package are the most common requests. HybriSlip™ plastic material is also stocked in thickness 0.18 mm.
HybriSlips™ are manufactured from rigid, nuclease free polycarbonate plastic which has a nominal thickness of 0.254 mm. Both surfaces are protected with a thin, removable layer of polyethylene film to prevent contamination. HybriSlips™ are packaged in quantities of 100 per bag in strips of 5 each. This product is available in a wide variety of square or rectangular dimensions and is easily customized to fit your specific application requirements.
Ideally suited for incubation steps where reagent volume is limited such as: In situ hybridization, in situ PCR, microarray hybridizations and immunohistochemistry. For best results, incubation steps should be performed in a humidified chamber to minimize evaporation from the edges. Where CV’s between replicate samples are important, distributing the same volume of reagent per cm2 can be a critical variable. HybriSlips™ are designed to overcome the limitations of do-it-yourself methods that involve trimming plastic films as covers.
Applications
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DNA Microarray
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Protein Microarray
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Immunohistochemistry
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In-situ Hybridization
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In-situ PCR
References
Ducreux, L. J. M., Morris, W. L., Hedley, P. E., Shepherd, T., Davies, H. V., Millam, S., & Taylor, M. A. (2005). Metabolic engineering of high carotenoid potato tubers containing enhanced levels of beta-carotene and lutein. Journal of Experimental Botany, 56(409), 81–89.
Savagner, P., Kusewitt, D. F., Carver, E. A., Magnino, F., Choi, C., Gridley, T., & Hudson, L. G. (2005). Developmental transcription factor slug is required for effective re-epithelialization by adult keratinocytes. Journal of Cellular Physiology, 202(3), 858–866.
Christianson, S., Golding, G. R., Campbell, J., Canadian Nosocomial Infection Surveillance Program, & Mulvey, M. R. (2007). Comparative genomics of Canadian epidemic lineages of methicillin-resistant Staphylococcus aureus. Journal of Clinical Microbiology, 45(6), 1904–1911.
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FAQ
Q: My hybridization cocktail contains Formamide , will HybriSlips™ work with this reagent?
A: Yes, HypriSlips™ are chemically resistant to most commonly used hybridization reagents. For a full list of chemical compatibilities you can consult many of the tables that are available on-line and search: polycarbonate.
Q: What is the best method to use for removing a HybriSlip™ from atop my specimen?
A: To minimize the surface tension between the HybriSlip™ and your specimen, we recommend dipping slides into a dish contain wash buffer and allowing the HybriSlip™ to slide off gently. Peeling them off, just as with glass coverslips, is not recommended as this can compromise sample adherence and morphology.
Q: How can I sterilize our HybriSlips™?
A: Using sterile technique, remove the liners from both surfaces of the product followed by one of the following options: dipping them into 70% isopropanol and allowing them to dry, using UV sterilization in the hood, or lightly flaming them and allowing them to cool before use. You may also autoclave the HybriSlips™ – first remove the liners (they melt).
Q: What is the refractive index of the polycarbonate?
A: The refractive index of the polycarbonate used to manufacture HybriSlips™ is 1.586
Q: What is the working temperature range for HybriSlip™ material?
A: Materials remain stable between minus 135 to 121oC
CoverWell™ incubation chambers are reusable, easy to apply chambers that attach without the use of adhesive. CoverWells™ enclose a large sample area with a small reagent volume and preserve kinetic (non-capillary) fluid dynamics for better reagent mixing and lower backgrounds within the chamber for more uniformly sensitive assays. These ready-to-use chambers are designed expressly for in situ hybridization and immunocytochemistry.
HybriWell™ Sealing System bonds securely to a microscope slide surface in seconds to confine small reagent volumes with samples and eliminate evaporation. Each package includes illustrated instructions for use, applicator and 200 adhesive port seals. Any of the designs listed may be easily customized to increase or decrease chamber depth. Hybriwells™ are recommended for most protein and nucleic acid assays. If you are using Cy5 or ® Alexa Fluor 647 direct-labeled DNA probes please refer to our new “fluorescent friendly” chambers. All current designs may be fabricated using our "fluorescent friendly" adhesive material.
Imaging spacers are ultra-thin adhesive spacers which peel-and-stick to coverglass or microscope slides to confine specimens without compression. Layer multiple spacers to custom build chambers to any depth desired. Thin, double-sided adhesive spacers confine specimens without compression. For high resolution microscopy, sandwich specimen and spacer between two No. 0 coverglass. Imaging spacers can be fabricated in different material and thickness for specific applications. The adhesive, also used to make Wound Splints, HybriWell and SecureSeal incubation chambers, is now available in sheets that may be purchased in a single layer thickness or prepared in multiple layers for your convenience.
SecureSeal™ Hybridization Chambers are thin, silicone-gasketed chambers providing optimal surface-to-volume fluid dynamics for hybridization assays on large or multiple specimens and microarrays on glass or film coated slides. These adhesive-backed chambers are designed for single-use, very-low volume incubations and are ideal for autoradiographic, fluorescent or chemiluminescent end-point applications requiring small, enclosed fluid volumes. SecureSeal™ Adhesive is a double sided tape with a silicone adhesive surface on one side (covered with a clear PET liner) and an acrylic adhesive on the other surface (covered with a paper liner).
