FastWells™ Reagent Barriers


FastWells™ are sticky, flexible silicone gaskets that form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Gaskets may be stacked to increase depth and volume.

FastWell™ Reagent Barriers provide rapid isolation of cells and tissues on slides or coverslips for antibody incubations. The barriers remain sealed during agitated washing steps, preventing reagents from spilling when shaken or rocked. FastWells™ peel off quickly and cleanly, leaving no residue to interfere with specimen cover-slipping. They can be washed, autoclaved and reused. The barriers can also be sealed to form incubation chambers using flexible HybriSlip™ covers.

Technical Resources


Instructions for Use - FastWells Reagent Barrier


  • Microscopy

  • Fluorescence In situ Hybridization (FISH)

  • Single-molecule fluorescence analysis

  • Immunohistochemistry


Silicone Isolator Application



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Yokomizo, T., Yamada-Inagawa, T., Yzaguirre, A. D., Chen, M. J., Speck, N. A., & Dzierzak, E. (2012). Whole-mount three-dimensional imaging of internally localized immunostained cells within mouse embryos. Nature Protocols, 7(3), 421–431.

Wang, Y.-H., Collins, A., Guo, L., Smith-Dupont, K. B., Gai, F., Svitkina, T., & Janmey, P. A. (2012). Divalent Cation-Induced Cluster Formation by Polyphosphoinositides in Model Membranes. Journal of the American Chemical Society, 134(7), 3387–3395. 

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