CS-16 CultureWell Chambered Coverglass Used To Validate A Reverse Genetic System For The Study of Orthobunyavirus

IN Cell Culture, CoverWell

Orthobunyavirus is a genus of the Peribunyaviridae family in the order Bunyavirales, currently comprising ~170 recognized viruses. Most orthobunyavirus species are transmitted by gnats and cause diseases in animals or humans. Their genomic organization is such that reassortment with related viruses is possible, resulting in altered virulence and zoonotic potential. Shuni virus (SHUV) is an orthobunyavirus related to Schmallenberg virus (SBV) that causes neurological disease in horses and birth defects in ruminants. Its association with neurological disease in humans suggests that its zoonotic potential should not be underestimated. J. Oymans et. al. developed a reverse genetic system to study the interaction of SHUV with different hosts and its ability to produce viable reassortants with other orthobunyaviruses.

In this study, an Immunofluorescence assay was performed to confirm the identity of the reassortants generated. For this experiment, Vera Cells cultured on CS-16 CultureWell™  Chambered Coverglass were infected with the virus, fixed with paraformaldehyde. Following incubation with antisera specific for SHUV and SBV, the cells were stained with fluorescently labeled secondary antibodies. CS-16 CultureWell™  Chambered Coverglass combines optimal cell growth characteristics with optical properties suitable for fluorescence and super-resolution microscopy.  The 16-well configuration is ideal to minimize the use of transfection reagents, antibodies, and fluorescent dyes proving to be a convenient, time-saving solution for in-vitro studies of viral infection.

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